Atomic force microscope images of lipid layers spread from vesicle suspensions

Inger Vikholm; Jouko Peltonen; Olle Teleman

doi:10.1016/0005-2736(94)00221-A

Atomic force microscope images of lipid layers spread from vesicle suspensions

Inger Vikholm (Corresponding Author), Jouko Peltonen, Olle Teleman

VTT Technical Research Centre of Finland

Åbo Akademi University

Research output: Contribution to journal › Article › Scientific › peer-review

28 Citations (Scopus)

Abstract

The layer formation of unilamellar vesicles of l-α-dimyristoyl phosphatidylcholine (DMPC) spread onto the air/liquid interface has been investigated. The layers were transferred to clean glass slides and onto slides made hydrophobic with multilayers of Cd arachidate. Aged vesicle suspensions aggregate during storage and are transferred as large domains as imaged with atomic force microscopy (AFM). Freshly prepared vesicles fuse and can be transferred as monolayers to hydrophobic supports. Furthermore, AFM images reveal the importance of positioning the solid support parallel to the moving barrier in order to obtain more uniform deposition of Cd arachidate.

Original language	English
Pages (from-to)	111-117
Journal	Biochimica et Biophysica Acta
Volume	1233
Issue number	2
DOIs	https://doi.org/10.1016/0005-2736(94)00221-A
Publication status	Published - 1995
MoE publication type	A1 Journal article-refereed

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title = "Atomic force microscope images of lipid layers spread from vesicle suspensions",

abstract = "The layer formation of unilamellar vesicles of l-α-dimyristoyl phosphatidylcholine (DMPC) spread onto the air/liquid interface has been investigated. The layers were transferred to clean glass slides and onto slides made hydrophobic with multilayers of Cd arachidate. Aged vesicle suspensions aggregate during storage and are transferred as large domains as imaged with atomic force microscopy (AFM). Freshly prepared vesicles fuse and can be transferred as monolayers to hydrophobic supports. Furthermore, AFM images reveal the importance of positioning the solid support parallel to the moving barrier in order to obtain more uniform deposition of Cd arachidate.",

author = "Inger Vikholm and Jouko Peltonen and Olle Teleman",

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AU - Peltonen, Jouko

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Y1 - 1995

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AB - The layer formation of unilamellar vesicles of l-α-dimyristoyl phosphatidylcholine (DMPC) spread onto the air/liquid interface has been investigated. The layers were transferred to clean glass slides and onto slides made hydrophobic with multilayers of Cd arachidate. Aged vesicle suspensions aggregate during storage and are transferred as large domains as imaged with atomic force microscopy (AFM). Freshly prepared vesicles fuse and can be transferred as monolayers to hydrophobic supports. Furthermore, AFM images reveal the importance of positioning the solid support parallel to the moving barrier in order to obtain more uniform deposition of Cd arachidate.

U2 - 10.1016/0005-2736(94)00221-A

DO - 10.1016/0005-2736(94)00221-A

M3 - Article

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EP - 117

JO - Biochimica et Biophysica Acta

JF - Biochimica et Biophysica Acta

IS - 2

ER -

Atomic force microscope images of lipid layers spread from vesicle suspensions

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