Atomic force microscope images of lipid layers spread from vesicle suspensions

Inger Vikholm (Corresponding Author), Jouko Peltonen, Olle Teleman

Research output: Contribution to journalArticleScientificpeer-review

28 Citations (Scopus)

Abstract

The layer formation of unilamellar vesicles of l-α-dimyristoyl phosphatidylcholine (DMPC) spread onto the air/liquid interface has been investigated. The layers were transferred to clean glass slides and onto slides made hydrophobic with multilayers of Cd arachidate. Aged vesicle suspensions aggregate during storage and are transferred as large domains as imaged with atomic force microscopy (AFM). Freshly prepared vesicles fuse and can be transferred as monolayers to hydrophobic supports. Furthermore, AFM images reveal the importance of positioning the solid support parallel to the moving barrier in order to obtain more uniform deposition of Cd arachidate.
Original languageEnglish
Pages (from-to)111-117
JournalBiochimica et Biophysica Acta
Volume1233
Issue number2
DOIs
Publication statusPublished - 1995
MoE publication typeA1 Journal article-refereed

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