Abstract
Colloidal probe microscopy has been used to study the interaction
between model cellulose surfaces and the role of cellulose binding domain
(CBD), peptides specifically binding to cellulose, in interfacial interaction
of cellulose surfaces modified with CBDs.
The interaction between pure cellulose surfaces in aqueous electrolyte
solution is dominated by double layer repulsive forces with the range and
magnitude of the net force dependent on electrolyte concentration. AFM imaging
reveals agglomeration of CBD adsorbed on cellulose surface. Despite an
increase in surface charge owing to CBD binding to cellulose surface, force
profiles are less repulsive for interactions involving, at least, one modified
surface. Such changes are attributed to irregularity of the topography of
protein surface and non-uniform distribution of surface charges on the surface
of modified cellulose.
Binding double CBD hybrid protein to cellulose surfaces causes adhesive forces
at retraction, whereas separation curves obtained with cellulose modified
with single CBD show small adhesion only at high ionic strength. This is
possibly caused by the formation of the cross-links between cellulose surfaces
in the case of double CBD.
Original language | English |
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Pages (from-to) | 125 - 135 |
Journal | Colloids and Surfaces B: Biointerfaces |
Volume | 35 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2004 |
MoE publication type | A1 Journal article-refereed |
Keywords
- AFM
- Colloid probe
- Cellulose surface
- Cellulose binding domain
- Force measurements