Atomic Resolution Structure of the HFBII Hydrophobin, a Self-assembling Amphiphile

Johanna Hakanpää, Arja Paananen, Sanna Askolin, Tiina Nakari-Setälä, Tarja Parkkinen, Merja Penttilä, Markus B. Linder, Juha Rouvinen

Research output: Contribution to journalArticleScientificpeer-review

188 Citations (Scopus)

Abstract

Hydrophobins are proteins specific to filamentous fungi. Hydrophobins have several important roles in fungal physiology, for example, adhesion, formation of protective surface coatings, and the reduction of the surface tension of water, which allows growth of aerial structures. Hydrophobins show remarkable biophysical properties, for example, they are the most powerful surface-active proteins known. To this point the molecular basis of the function of this group of proteins has been largely unknown. We have now determined the crystal structure of the hydrophobin HFBII from Trichoderma reesei at 1.0 Å resolution. HFBII has a novel, compact single domain structure containing one α-helix and four antiparallel β-strands that completely envelop two disulfide bridges. The protein surface is mainly hydrophilic, but two β-hairpin loops contain several conserved aliphatic side chains that form a flat hydrophobic patch that makes the molecule amphiphilic. The amphiphilicity of the HFBII molecule is expected to be a source for surface activity, and we suggest that the behavior of this surfactant is greatly enhanced by the self-assembly that is favored by the combination of size and rigidity. This mechanism of function is supported by atomic force micrographs that show highly ordered arrays of HFBII at the air water interface. The data presented show that much of the current views on structure function relations in hydrophobins must be re-evaluated.

Original languageEnglish
Pages (from-to)534-539
Number of pages6
JournalJournal of Biological Chemistry
Volume279
Issue number1
DOIs
Publication statusPublished - 2 Jan 2004
MoE publication typeA1 Journal article-refereed

Fingerprint

Amphiphiles
Membrane Proteins
Trichoderma
Surface Tension
Water
Surface-Active Agents
Disulfides
Proteins
Molecules
Fungi
Air
Physiology
Rigidity
Self assembly
Surface tension
Adhesion
Growth
Crystal structure
Antennas
Coatings

Keywords

  • filamentous fungi
  • hydrophobins
  • crystal structure
  • molecular modelling

Cite this

Hakanpää, Johanna ; Paananen, Arja ; Askolin, Sanna ; Nakari-Setälä, Tiina ; Parkkinen, Tarja ; Penttilä, Merja ; Linder, Markus B. ; Rouvinen, Juha. / Atomic Resolution Structure of the HFBII Hydrophobin, a Self-assembling Amphiphile. In: Journal of Biological Chemistry. 2004 ; Vol. 279, No. 1. pp. 534-539.
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Atomic Resolution Structure of the HFBII Hydrophobin, a Self-assembling Amphiphile. / Hakanpää, Johanna; Paananen, Arja; Askolin, Sanna; Nakari-Setälä, Tiina; Parkkinen, Tarja; Penttilä, Merja; Linder, Markus B.; Rouvinen, Juha.

In: Journal of Biological Chemistry, Vol. 279, No. 1, 02.01.2004, p. 534-539.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Atomic Resolution Structure of the HFBII Hydrophobin, a Self-assembling Amphiphile

AU - Hakanpää, Johanna

AU - Paananen, Arja

AU - Askolin, Sanna

AU - Nakari-Setälä, Tiina

AU - Parkkinen, Tarja

AU - Penttilä, Merja

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