Avian model for B-cell immunology

New genomes and phylotranscriptomics

Pekka Kohonen, K. P. Nera, O. Lassila

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

The purpose of this review is to discuss the use of chicken and other model organisms in the study of B-cell development and function as well as to highlight the opportunities afforded by the expanded genome-sequencing efforts. A brief introduction on chicken B-cell biology is followed by discussion of somatic cell reverse genetic approaches using the DT40 cell line. The unique advantages of the DT40 system are emphasized with discussion on B-cell receptor signalling research as well as on DNA repair and mechanisms of immunoglobulin diversification. An attempt is made to compare and contrast the results from chicken with mouse knockouts on the one hand and RNAi with human cell lines on the other. Chicken is also emerging strongly as a platform for gene expression analysis, and avian studies are compared with mammalian studies. Multi-species gene co-expression analysis, which could also be termed phylotranscriptomics, aims to use the evolutionary distance between organisms to its advantage. This approach, still in its infancy, is also reviewed and its applicability to the chicken is discussed.
Original languageEnglish
Pages (from-to)113-121
JournalScandinavian Journal of Immunology
Volume66
Issue number2-3
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Fingerprint

Allergy and Immunology
Chickens
B-Lymphocytes
Genome
Gene Expression
Reverse Genetics
Cell Line
RNA Interference
Knockout Mice
DNA Repair
Cell Biology
Immunoglobulins
Research

Cite this

Kohonen, Pekka ; Nera, K. P. ; Lassila, O. / Avian model for B-cell immunology : New genomes and phylotranscriptomics. In: Scandinavian Journal of Immunology. 2007 ; Vol. 66, No. 2-3. pp. 113-121.
@article{9710b2c000d647a1b4bd322437233373,
title = "Avian model for B-cell immunology: New genomes and phylotranscriptomics",
abstract = "The purpose of this review is to discuss the use of chicken and other model organisms in the study of B-cell development and function as well as to highlight the opportunities afforded by the expanded genome-sequencing efforts. A brief introduction on chicken B-cell biology is followed by discussion of somatic cell reverse genetic approaches using the DT40 cell line. The unique advantages of the DT40 system are emphasized with discussion on B-cell receptor signalling research as well as on DNA repair and mechanisms of immunoglobulin diversification. An attempt is made to compare and contrast the results from chicken with mouse knockouts on the one hand and RNAi with human cell lines on the other. Chicken is also emerging strongly as a platform for gene expression analysis, and avian studies are compared with mammalian studies. Multi-species gene co-expression analysis, which could also be termed phylotranscriptomics, aims to use the evolutionary distance between organisms to its advantage. This approach, still in its infancy, is also reviewed and its applicability to the chicken is discussed.",
author = "Pekka Kohonen and Nera, {K. P.} and O. Lassila",
year = "2007",
doi = "10.1111/j.1365-3083.2007.01973.x",
language = "English",
volume = "66",
pages = "113--121",
journal = "Scandinavian Journal of Immunology",
issn = "0300-9475",
publisher = "Wiley-Blackwell",
number = "2-3",

}

Avian model for B-cell immunology : New genomes and phylotranscriptomics. / Kohonen, Pekka; Nera, K. P.; Lassila, O.

In: Scandinavian Journal of Immunology, Vol. 66, No. 2-3, 2007, p. 113-121.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Avian model for B-cell immunology

T2 - New genomes and phylotranscriptomics

AU - Kohonen, Pekka

AU - Nera, K. P.

AU - Lassila, O.

PY - 2007

Y1 - 2007

N2 - The purpose of this review is to discuss the use of chicken and other model organisms in the study of B-cell development and function as well as to highlight the opportunities afforded by the expanded genome-sequencing efforts. A brief introduction on chicken B-cell biology is followed by discussion of somatic cell reverse genetic approaches using the DT40 cell line. The unique advantages of the DT40 system are emphasized with discussion on B-cell receptor signalling research as well as on DNA repair and mechanisms of immunoglobulin diversification. An attempt is made to compare and contrast the results from chicken with mouse knockouts on the one hand and RNAi with human cell lines on the other. Chicken is also emerging strongly as a platform for gene expression analysis, and avian studies are compared with mammalian studies. Multi-species gene co-expression analysis, which could also be termed phylotranscriptomics, aims to use the evolutionary distance between organisms to its advantage. This approach, still in its infancy, is also reviewed and its applicability to the chicken is discussed.

AB - The purpose of this review is to discuss the use of chicken and other model organisms in the study of B-cell development and function as well as to highlight the opportunities afforded by the expanded genome-sequencing efforts. A brief introduction on chicken B-cell biology is followed by discussion of somatic cell reverse genetic approaches using the DT40 cell line. The unique advantages of the DT40 system are emphasized with discussion on B-cell receptor signalling research as well as on DNA repair and mechanisms of immunoglobulin diversification. An attempt is made to compare and contrast the results from chicken with mouse knockouts on the one hand and RNAi with human cell lines on the other. Chicken is also emerging strongly as a platform for gene expression analysis, and avian studies are compared with mammalian studies. Multi-species gene co-expression analysis, which could also be termed phylotranscriptomics, aims to use the evolutionary distance between organisms to its advantage. This approach, still in its infancy, is also reviewed and its applicability to the chicken is discussed.

U2 - 10.1111/j.1365-3083.2007.01973.x

DO - 10.1111/j.1365-3083.2007.01973.x

M3 - Article

VL - 66

SP - 113

EP - 121

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

SN - 0300-9475

IS - 2-3

ER -