Bacillus amyloliquefaciens — Production Host for Industrial Enzymes

Jari Vehmaanperä

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleProfessional

Abstract

Bacillus amyloliquefaciens is a widely used host for production of industrial enzymes (Aunstrup et al. 1979). However, the species does not become physiologically competent for DNA transformation, unlike the related species B. subtilis. Therefore, alternative methods for introduction of plasmids into B. amyloliquefaciens have been developed. These include the protoplast transformation (Vehmaanperä 1988) and the electroporation methods (Vehmaanperä 1989). The latter is described below.
Original languageEnglish
Title of host publicationElectrotransformation of Bacteria
EditorsNatalie Eynard, Justin Teissié
Place of PublicationHeidelberg
PublisherSpringer
Chapter14
Pages119-123
ISBN (Electronic)978-3-662-04305-9
ISBN (Print)978-3-642-08593-2
DOIs
Publication statusPublished - 2000
MoE publication typeD2 Article in professional manuals or guides or professional information systems or text book material

Publication series

SeriesSpringer Lab Manuals (SLM)

Fingerprint

Bacillus amyloliquefaciens
electroporation
enzymes
protoplasts
plasmids
DNA
methodology

Cite this

Vehmaanperä, J. (2000). Bacillus amyloliquefaciens — Production Host for Industrial Enzymes. In N. Eynard, & J. Teissié (Eds.), Electrotransformation of Bacteria (pp. 119-123). Heidelberg: Springer. Springer Lab Manuals (SLM) https://doi.org/10.1007/978-3-662-04305-9_14
Vehmaanperä, Jari. / Bacillus amyloliquefaciens — Production Host for Industrial Enzymes. Electrotransformation of Bacteria. editor / Natalie Eynard ; Justin Teissié. Heidelberg : Springer, 2000. pp. 119-123 (Springer Lab Manuals (SLM)).
@inbook{0a31a5cff7704161a0c8554b243f37d3,
title = "Bacillus amyloliquefaciens — Production Host for Industrial Enzymes",
abstract = "Bacillus amyloliquefaciens is a widely used host for production of industrial enzymes (Aunstrup et al. 1979). However, the species does not become physiologically competent for DNA transformation, unlike the related species B. subtilis. Therefore, alternative methods for introduction of plasmids into B. amyloliquefaciens have been developed. These include the protoplast transformation (Vehmaanper{\"a} 1988) and the electroporation methods (Vehmaanper{\"a} 1989). The latter is described below.",
author = "Jari Vehmaanper{\"a}",
year = "2000",
doi = "10.1007/978-3-662-04305-9_14",
language = "English",
isbn = "978-3-642-08593-2",
series = "Springer Lab Manuals (SLM)",
publisher = "Springer",
pages = "119--123",
editor = "Eynard, {Natalie } and Teissi{\'e}, {Justin }",
booktitle = "Electrotransformation of Bacteria",
address = "Germany",

}

Vehmaanperä, J 2000, Bacillus amyloliquefaciens — Production Host for Industrial Enzymes. in N Eynard & J Teissié (eds), Electrotransformation of Bacteria. Springer, Heidelberg, Springer Lab Manuals (SLM), pp. 119-123. https://doi.org/10.1007/978-3-662-04305-9_14

Bacillus amyloliquefaciens — Production Host for Industrial Enzymes. / Vehmaanperä, Jari.

Electrotransformation of Bacteria. ed. / Natalie Eynard; Justin Teissié. Heidelberg : Springer, 2000. p. 119-123 (Springer Lab Manuals (SLM)).

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleProfessional

TY - CHAP

T1 - Bacillus amyloliquefaciens — Production Host for Industrial Enzymes

AU - Vehmaanperä, Jari

PY - 2000

Y1 - 2000

N2 - Bacillus amyloliquefaciens is a widely used host for production of industrial enzymes (Aunstrup et al. 1979). However, the species does not become physiologically competent for DNA transformation, unlike the related species B. subtilis. Therefore, alternative methods for introduction of plasmids into B. amyloliquefaciens have been developed. These include the protoplast transformation (Vehmaanperä 1988) and the electroporation methods (Vehmaanperä 1989). The latter is described below.

AB - Bacillus amyloliquefaciens is a widely used host for production of industrial enzymes (Aunstrup et al. 1979). However, the species does not become physiologically competent for DNA transformation, unlike the related species B. subtilis. Therefore, alternative methods for introduction of plasmids into B. amyloliquefaciens have been developed. These include the protoplast transformation (Vehmaanperä 1988) and the electroporation methods (Vehmaanperä 1989). The latter is described below.

U2 - 10.1007/978-3-662-04305-9_14

DO - 10.1007/978-3-662-04305-9_14

M3 - Chapter or book article

SN - 978-3-642-08593-2

T3 - Springer Lab Manuals (SLM)

SP - 119

EP - 123

BT - Electrotransformation of Bacteria

A2 - Eynard, Natalie

A2 - Teissié, Justin

PB - Springer

CY - Heidelberg

ER -

Vehmaanperä J. Bacillus amyloliquefaciens — Production Host for Industrial Enzymes. In Eynard N, Teissié J, editors, Electrotransformation of Bacteria. Heidelberg: Springer. 2000. p. 119-123. (Springer Lab Manuals (SLM)). https://doi.org/10.1007/978-3-662-04305-9_14