Bacillus amyloliquefaciens — Production Host for Industrial Enzymes

Jari Vehmaanperä

doi:10.1007/978-3-662-04305-9_14

Bacillus amyloliquefaciens — Production Host for Industrial Enzymes

Jari Vehmaanperä

VTT Technical Research Centre of Finland

Research output: Chapter in Book/Report/Conference proceeding › Chapter or book article › Professional

Abstract

Bacillus amyloliquefaciens is a widely used host for production of industrial enzymes (Aunstrup et al. 1979). However, the species does not become physiologically competent for DNA transformation, unlike the related species B. subtilis. Therefore, alternative methods for introduction of plasmids into B. amyloliquefaciens have been developed. These include the protoplast transformation (Vehmaanperä 1988) and the electroporation methods (Vehmaanperä 1989). The latter is described below.

Original language	English
Title of host publication	Electrotransformation of Bacteria
Editors	Natalie Eynard, Justin Teissié
Place of Publication	Heidelberg
Publisher	Springer
Chapter	14
Pages	119-123
ISBN (Electronic)	978-3-662-04305-9
ISBN (Print)	978-3-642-08593-2
DOIs	https://doi.org/10.1007/978-3-662-04305-9_14
Publication status	Published - 2000
MoE publication type	D2 Article in professional manuals or guides or professional information systems or text book material

Publication series

Series	Springer Lab Manuals

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Cite this

Vehmaanperä, J. (2000). Bacillus amyloliquefaciens — Production Host for Industrial Enzymes. In N. Eynard, & J. Teissié (Eds.), Electrotransformation of Bacteria (pp. 119-123). Springer. Springer Lab Manuals https://doi.org/10.1007/978-3-662-04305-9_14

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AB - Bacillus amyloliquefaciens is a widely used host for production of industrial enzymes (Aunstrup et al. 1979). However, the species does not become physiologically competent for DNA transformation, unlike the related species B. subtilis. Therefore, alternative methods for introduction of plasmids into B. amyloliquefaciens have been developed. These include the protoplast transformation (Vehmaanperä 1988) and the electroporation methods (Vehmaanperä 1989). The latter is described below.

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Access to Document

10.1007/978-3-662-04305-9_14