Bacterial populations on brewery filling hall surfaces as revealed by next-generation sequencing

Outi Priha (Corresponding Author), Mari Raulio, Johanna Maukonen, Anna-Kaisa Vehviläinen, Erna Storgårds

    Research output: Contribution to journalArticleScientificpeer-review

    5 Citations (Scopus)


    Due to the presence of moisture and nutrients, brewery filling line surfaces are susceptible to unwanted microbial attachment. Knowledge of the attaching microbes will aid in designing hygienic control of the process. In this study the bacterial diversity present on brewery filling line surfaces was revealed by next generation sequencing. The two filling lines studied maintained their characteristic bacterial community throughout three sampling times (13-163 days). On the glass bottle line, ?-proteobacteria dominated (35-82% of all OTUs), whereas on the canning line a-, ß- and ?-proteobacteria and actinobacteria were most common. The most frequently detected genera were Acinetobacter, Propinobacterium and Pseudomonas. The halophilic genus Halomonas was commonly detected, which might be due to its tolerance to alkaline foam cleaners. This study has revealed a detailed overall picture of the bacterial groups present on filling line surfaces. Further effort should be given to determine the efficacy of washing procedures on different bacterial groups.
    Original languageEnglish
    Pages (from-to)571-581
    Issue number5
    Publication statusPublished - 2016
    MoE publication typeA1 Journal article-refereed


    • process hygiene
    • filling line
    • bacterial community
    • next generation sequencing
    • NGS
    • illumina MiSeq


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