BACKGROUND: Various studies have suggested the involvement of infectious agents in chronic inflammatory diseases, including atherosclerosis. By using a novel subtraction broad-range PCR approach, we defined bacterial DNA signatures in surgically removed sterile abdominal aorta samples of patients with aortic atherosclerosis.
METHODS: Partial bacterial 16S rDNA nucleotide sequences were determined using broad-range PCR from aortic samples of 20 patients, and from appropriate methodological controls. In all, 160 sequences from 16 clone libraries were studied.
RESULTS: After subtraction analysis 16 clinically relevant bacterial sequence-types were identified among the patient samples, whereas 29 were discarded as potential methodological contaminants. On average 2.2+/-1.2 different bacterial sequence-types were present in the nine true PCR-positive atheroma samples.
CONCLUSIONS: Many studies have reported the presence of a variety of bacterial sequences from atherosclerotic lesions. However, the results obtained with these PCR technologies may have been skewed by methodological contaminants. After our subtraction approach, 63% of the remaining sequence-types from sites of aortic atherosclerosis were related to those of known human pathogens. This may imply that advanced atherosclerotic plaques accumulate bacterial DNA.
|Number of pages||6|
|Publication status||Published - Nov 2008|
|MoE publication type||A1 Journal article-refereed|
- Aorta, Abdominal
- Aortic Aneurysm, Abdominal/microbiology
- Aortic Diseases/microbiology
- DNA, Bacterial/isolation & purification
- DNA, Ribosomal/isolation & purification
- Middle Aged
- Polymerase Chain Reaction/methods
- Predictive Value of Tests
- RNA, Ribosomal, 16S/genetics
- Sequence Analysis, DNA/methods