Baculoviral display of functional scFv and synthetic IgG-binding domains

David Mottershead, Kaija Alfthan, Kirsi Ojala, Kristiina Takkinen, Christian Oker-Blom (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

42 Citations (Scopus)

Abstract

Viral vectors displaying specific ligand binding moities such as scFv fragments or intact antibodies hold promise for the development of targeted gene therapy vectors. In this report we describe baculoviral vectors displaying either functional scFv fragments or the synthetic Z/ZZ IgG binding domain derived from protein A. Display on the baculovirus surface was achieved via fusion of the scFv fragment or Z/ZZ domain to the N-terminus of gp64, the major envelope protein of the Autographa californica nuclear polyhedrosis virus, AcNPV. As examples of scFv fragments we have used a murine scFv specific for the hapten 2-phenyloxazolone and a human scFv specific for carcinoembryonic antigen. In principle, the Z/ZZ IgG binding domain displaying baculoviruses could be targeted to specific cell types via the binding of an appropriate antibody. We envisage applications for scFv and Z/ZZ domain displaying baculoviral vectors in the gene therapy field.

Original languageEnglish
Pages (from-to)84 - 90
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume275
Issue number1
DOIs
Publication statusPublished - 2000
MoE publication typeA1 Journal article-refereed

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Baculoviridae
Genetic Therapy
Immunoglobulin G
Display devices
Nucleopolyhedrovirus
Gene therapy
Antibodies
Haptens
Carcinoembryonic Antigen
Staphylococcal Protein A
Ligands
Viruses
Proteins
Fusion reactions
2-phenyloxazolone

Cite this

Mottershead, David ; Alfthan, Kaija ; Ojala, Kirsi ; Takkinen, Kristiina ; Oker-Blom, Christian. / Baculoviral display of functional scFv and synthetic IgG-binding domains. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 275, No. 1. pp. 84 - 90.
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Baculoviral display of functional scFv and synthetic IgG-binding domains. / Mottershead, David; Alfthan, Kaija; Ojala, Kirsi; Takkinen, Kristiina; Oker-Blom, Christian (Corresponding Author).

In: Biochemical and Biophysical Research Communications, Vol. 275, No. 1, 2000, p. 84 - 90.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Mottershead, David

AU - Alfthan, Kaija

AU - Ojala, Kirsi

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AU - Oker-Blom, Christian

PY - 2000

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AB - Viral vectors displaying specific ligand binding moities such as scFv fragments or intact antibodies hold promise for the development of targeted gene therapy vectors. In this report we describe baculoviral vectors displaying either functional scFv fragments or the synthetic Z/ZZ IgG binding domain derived from protein A. Display on the baculovirus surface was achieved via fusion of the scFv fragment or Z/ZZ domain to the N-terminus of gp64, the major envelope protein of the Autographa californica nuclear polyhedrosis virus, AcNPV. As examples of scFv fragments we have used a murine scFv specific for the hapten 2-phenyloxazolone and a human scFv specific for carcinoembryonic antigen. In principle, the Z/ZZ IgG binding domain displaying baculoviruses could be targeted to specific cell types via the binding of an appropriate antibody. We envisage applications for scFv and Z/ZZ domain displaying baculoviral vectors in the gene therapy field.

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