Baculoviral display of functional scFv and synthetic IgG-binding domains

David Mottershead, Kaija Alfthan, Kirsi Ojala, Kristiina Takkinen, Christian Oker-Blom (Corresponding Author)

    Research output: Contribution to journalArticleScientificpeer-review

    43 Citations (Scopus)

    Abstract

    Viral vectors displaying specific ligand binding moities such as scFv fragments or intact antibodies hold promise for the development of targeted gene therapy vectors. In this report we describe baculoviral vectors displaying either functional scFv fragments or the synthetic Z/ZZ IgG binding domain derived from protein A. Display on the baculovirus surface was achieved via fusion of the scFv fragment or Z/ZZ domain to the N-terminus of gp64, the major envelope protein of the Autographa californica nuclear polyhedrosis virus, AcNPV. As examples of scFv fragments we have used a murine scFv specific for the hapten 2-phenyloxazolone and a human scFv specific for carcinoembryonic antigen. In principle, the Z/ZZ IgG binding domain displaying baculoviruses could be targeted to specific cell types via the binding of an appropriate antibody. We envisage applications for scFv and Z/ZZ domain displaying baculoviral vectors in the gene therapy field.

    Original languageEnglish
    Pages (from-to)84 - 90
    Number of pages7
    JournalBiochemical and Biophysical Research Communications
    Volume275
    Issue number1
    DOIs
    Publication statusPublished - 2000
    MoE publication typeA1 Journal article-refereed

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