TY - JOUR
T1 - Baculoviral display of the green fluorescent protein and rubella virus envelope proteins
AU - Mottershead, David
AU - Van der Linden, Inge
AU - von Bonsdorff, Carl-Henrik
AU - Keinänen, Kari
AU - Oker-Blom, Christian
PY - 1997
Y1 - 1997
N2 - The ability to
display heterologous proteins and peptides on the surface of different
types of bacteriophage has proven extremely useful in protein
structure/function studies. To display such proteins in a eucaryotic
environment, we have produced a vector allowing for fusion of proteins
to the amino-terminus of theAutographa californicanuclear
polyhedrosis virus (AcNPV) major envelope glycoprotein, gp64. Such
fusion proteins incorporate into the baculoviral virion and display the
FLAG epitope tag. We have further produced recombinant baculoviruses
displaying the green fluorescent protein (GFP) and the rubella virus
envelope proteins, E1 and E2. The incorporation of the GFPgp64, E1gp64,
and E2gp64 fusion proteins into the baculovirus particle was
demonstrated by western blot analysis of purified budded virus. This is
the first report of the display of the GFP protein or the individual
rubella virus spike proteins on the surface of an enveloped virus. Such a
eucaryotic viral display system may be useful for the display of
proteins dependent on glycosylation for activity and for targeting of
recombinant baculoviruses to novel host cell types as a gene transfer
vehicle.
AB - The ability to
display heterologous proteins and peptides on the surface of different
types of bacteriophage has proven extremely useful in protein
structure/function studies. To display such proteins in a eucaryotic
environment, we have produced a vector allowing for fusion of proteins
to the amino-terminus of theAutographa californicanuclear
polyhedrosis virus (AcNPV) major envelope glycoprotein, gp64. Such
fusion proteins incorporate into the baculoviral virion and display the
FLAG epitope tag. We have further produced recombinant baculoviruses
displaying the green fluorescent protein (GFP) and the rubella virus
envelope proteins, E1 and E2. The incorporation of the GFPgp64, E1gp64,
and E2gp64 fusion proteins into the baculovirus particle was
demonstrated by western blot analysis of purified budded virus. This is
the first report of the display of the GFP protein or the individual
rubella virus spike proteins on the surface of an enveloped virus. Such a
eucaryotic viral display system may be useful for the display of
proteins dependent on glycosylation for activity and for targeting of
recombinant baculoviruses to novel host cell types as a gene transfer
vehicle.
U2 - 10.1006/bbrc.1997.7372
DO - 10.1006/bbrc.1997.7372
M3 - Article
SN - 0006-291X
VL - 238
SP - 717
EP - 722
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -