Berry phenolics: Antimicrobial properties and mechanisms of action against severe human pathogens

Liisa Nohynek, Hanna-Leena Alakomi, Marja Kähkönen, Marina Heinonen, Ilkka Helander, Kirsi-Marja Oksman-Caldentey, Riitta Puupponen-Pimiä

Research output: Contribution to journalArticleScientificpeer-review

270 Citations (Scopus)

Abstract

Abstract: Antimicrobial activity and mechanisms of phenolic extracts of 12 Nordic berries were studied against selected human pathogenic microbes. The most sensitive bacteria on berry phenolics were Helicobacter pylori and Bacillus cereus. Campylobacter jejuni and Candida albicans were inhibited only with phenolic extracts of cloudberry, raspberry, and strawberry, which all were rich in ellagitannins. Cloudberry extract gave strong microbicidic effects on the basis of plate count with all studied strains. However, fluorescence staining of liquid cultures of virulent Salmonella showed viable cells not detectable by plate count adhering to cloudberry extract, whereas Staphylococcus aureus cells adhered to berry extracts were dead on the basis of their fluorescence and plate count. Phenolic extracts of cloudberry and raspberry disintegrated the outer membrane of examined Salmonella strains as indicated by 1-N-phenylnaphthylamine (NPN) uptake increase and analysis of liberation of [14C]galactose- lipopolysaccharide. Gallic acid effectively permeabilized the tested Salmonella strains, and significant increase in the NPN uptake was recorded. The stability of berry phenolics and their antimicrobial activity in berries stored frozen for a year were examined using Escherichia coli and nonvirulent Salmonella enterica sv. Typhimurium. The amount of phenolic compounds decreased in all berries, but their antimicrobial activity was not influenced accordingly. Cloudberry, in particular, showed constantly strong antimicrobial activity during the storage.
Original languageEnglish
Pages (from-to)18-32
Number of pages15
JournalNutrition and Cancer
Volume54
Issue number1
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

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Fruit
N-phenyl-1-naphthylamine
Salmonella
Fluorescence
Hydrolyzable Tannins
Fragaria
Gallic Acid
Campylobacter jejuni
Bacillus cereus
Salmonella enterica
Candida albicans
Galactose
Helicobacter pylori
Lipopolysaccharides
Staphylococcus aureus
Staining and Labeling
Escherichia coli
Bacteria
Membranes
Rubus

Cite this

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title = "Berry phenolics: Antimicrobial properties and mechanisms of action against severe human pathogens",
abstract = "Abstract: Antimicrobial activity and mechanisms of phenolic extracts of 12 Nordic berries were studied against selected human pathogenic microbes. The most sensitive bacteria on berry phenolics were Helicobacter pylori and Bacillus cereus. Campylobacter jejuni and Candida albicans were inhibited only with phenolic extracts of cloudberry, raspberry, and strawberry, which all were rich in ellagitannins. Cloudberry extract gave strong microbicidic effects on the basis of plate count with all studied strains. However, fluorescence staining of liquid cultures of virulent Salmonella showed viable cells not detectable by plate count adhering to cloudberry extract, whereas Staphylococcus aureus cells adhered to berry extracts were dead on the basis of their fluorescence and plate count. Phenolic extracts of cloudberry and raspberry disintegrated the outer membrane of examined Salmonella strains as indicated by 1-N-phenylnaphthylamine (NPN) uptake increase and analysis of liberation of [14C]galactose- lipopolysaccharide. Gallic acid effectively permeabilized the tested Salmonella strains, and significant increase in the NPN uptake was recorded. The stability of berry phenolics and their antimicrobial activity in berries stored frozen for a year were examined using Escherichia coli and nonvirulent Salmonella enterica sv. Typhimurium. The amount of phenolic compounds decreased in all berries, but their antimicrobial activity was not influenced accordingly. Cloudberry, in particular, showed constantly strong antimicrobial activity during the storage.",
author = "Liisa Nohynek and Hanna-Leena Alakomi and Marja K{\"a}hk{\"o}nen and Marina Heinonen and Ilkka Helander and Kirsi-Marja Oksman-Caldentey and Riitta Puupponen-Pimi{\"a}",
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Berry phenolics : Antimicrobial properties and mechanisms of action against severe human pathogens. / Nohynek, Liisa; Alakomi, Hanna-Leena; Kähkönen, Marja; Heinonen, Marina; Helander, Ilkka; Oksman-Caldentey, Kirsi-Marja; Puupponen-Pimiä, Riitta.

In: Nutrition and Cancer, Vol. 54, No. 1, 2006, p. 18-32.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Berry phenolics

T2 - Antimicrobial properties and mechanisms of action against severe human pathogens

AU - Nohynek, Liisa

AU - Alakomi, Hanna-Leena

AU - Kähkönen, Marja

AU - Heinonen, Marina

AU - Helander, Ilkka

AU - Oksman-Caldentey, Kirsi-Marja

AU - Puupponen-Pimiä, Riitta

PY - 2006

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N2 - Abstract: Antimicrobial activity and mechanisms of phenolic extracts of 12 Nordic berries were studied against selected human pathogenic microbes. The most sensitive bacteria on berry phenolics were Helicobacter pylori and Bacillus cereus. Campylobacter jejuni and Candida albicans were inhibited only with phenolic extracts of cloudberry, raspberry, and strawberry, which all were rich in ellagitannins. Cloudberry extract gave strong microbicidic effects on the basis of plate count with all studied strains. However, fluorescence staining of liquid cultures of virulent Salmonella showed viable cells not detectable by plate count adhering to cloudberry extract, whereas Staphylococcus aureus cells adhered to berry extracts were dead on the basis of their fluorescence and plate count. Phenolic extracts of cloudberry and raspberry disintegrated the outer membrane of examined Salmonella strains as indicated by 1-N-phenylnaphthylamine (NPN) uptake increase and analysis of liberation of [14C]galactose- lipopolysaccharide. Gallic acid effectively permeabilized the tested Salmonella strains, and significant increase in the NPN uptake was recorded. The stability of berry phenolics and their antimicrobial activity in berries stored frozen for a year were examined using Escherichia coli and nonvirulent Salmonella enterica sv. Typhimurium. The amount of phenolic compounds decreased in all berries, but their antimicrobial activity was not influenced accordingly. Cloudberry, in particular, showed constantly strong antimicrobial activity during the storage.

AB - Abstract: Antimicrobial activity and mechanisms of phenolic extracts of 12 Nordic berries were studied against selected human pathogenic microbes. The most sensitive bacteria on berry phenolics were Helicobacter pylori and Bacillus cereus. Campylobacter jejuni and Candida albicans were inhibited only with phenolic extracts of cloudberry, raspberry, and strawberry, which all were rich in ellagitannins. Cloudberry extract gave strong microbicidic effects on the basis of plate count with all studied strains. However, fluorescence staining of liquid cultures of virulent Salmonella showed viable cells not detectable by plate count adhering to cloudberry extract, whereas Staphylococcus aureus cells adhered to berry extracts were dead on the basis of their fluorescence and plate count. Phenolic extracts of cloudberry and raspberry disintegrated the outer membrane of examined Salmonella strains as indicated by 1-N-phenylnaphthylamine (NPN) uptake increase and analysis of liberation of [14C]galactose- lipopolysaccharide. Gallic acid effectively permeabilized the tested Salmonella strains, and significant increase in the NPN uptake was recorded. The stability of berry phenolics and their antimicrobial activity in berries stored frozen for a year were examined using Escherichia coli and nonvirulent Salmonella enterica sv. Typhimurium. The amount of phenolic compounds decreased in all berries, but their antimicrobial activity was not influenced accordingly. Cloudberry, in particular, showed constantly strong antimicrobial activity during the storage.

U2 - 10.1207/s15327914nc5401_4

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JO - Nutrition and Cancer

JF - Nutrition and Cancer

SN - 0163-5581

IS - 1

ER -