Bimolecular fluorescence complementation (BiFC) technique in yeast Saccharomyces cerevisiae and mammalian cells

Marion Weber-Boyvat, Shiqian Li, Kari-Pekka Skarp, Vesa M. Olkkonen, Daoguang Yan, Jussi Jäntti

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleProfessional

4 Citations (Scopus)

Abstract

Visualization of protein-protein interactions in vivo offers a powerful tool to resolve spatial and temporal aspects of cellular functions. The bimolecular fluorescence complementation (BiFC) makes use of nonfluorescent fragments of green fluorescent protein or its variants that are added as "tags" to target proteins under study. Only upon target protein interaction is a fluorescent protein complex assembled, and the site of interaction can be monitored by microscopy. In this chapter, we describe the method and tools for the use of BiFC in the yeast Saccharomyces cerevisiae and in mammalian cells.
Original languageEnglish
Title of host publicationMembrane Trafficking
EditorsBor Luen Tang
PublisherHumana Press
Pages277-288
EditionSecond Edition
ISBN (Electronic)978-1-4939-2309-0
ISBN (Print)978-1-4939-2308-3
DOIs
Publication statusPublished - 2015
MoE publication typeD2 Article in professional manuals or guides or professional information systems or text book material

Publication series

SeriesMethods in Molecular Biology
Volume1270
ISSN1064-3745

Keywords

  • Bimolecular fluorescence complementation
  • EYFP
  • Fluorescence microscopy
  • GFP
  • Venus
  • Protein interactions
  • Saccharomyces cerevisiae
  • Yeast
  • Mammalian

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    Weber-Boyvat, M., Li, S., Skarp, K-P., Olkkonen, V. M., Yan, D., & Jäntti, J. (2015). Bimolecular fluorescence complementation (BiFC) technique in yeast Saccharomyces cerevisiae and mammalian cells. In B. L. Tang (Ed.), Membrane Trafficking (Second Edition ed., pp. 277-288). Humana Press. Methods in Molecular Biology, Vol.. 1270 https://doi.org/10.1007/978-1-4939-2309-0_20