Bimolecular fluorescence complementation (BiFC) technique in yeast Saccharomyces cerevisiae and mammalian cells

Marion Weber-Boyvat, Shiqian Li, Kari-Pekka Skarp, Vesa M. Olkkonen, Daoguang Yan, Jussi Jäntti

    Research output: Chapter in Book/Report/Conference proceedingChapter or book articleProfessional

    4 Citations (Scopus)

    Abstract

    Visualization of protein-protein interactions in vivo offers a powerful tool to resolve spatial and temporal aspects of cellular functions. The bimolecular fluorescence complementation (BiFC) makes use of nonfluorescent fragments of green fluorescent protein or its variants that are added as "tags" to target proteins under study. Only upon target protein interaction is a fluorescent protein complex assembled, and the site of interaction can be monitored by microscopy. In this chapter, we describe the method and tools for the use of BiFC in the yeast Saccharomyces cerevisiae and in mammalian cells.
    Original languageEnglish
    Title of host publicationMembrane Trafficking
    EditorsBor Luen Tang
    PublisherHumana Press
    Pages277-288
    EditionSecond Edition
    ISBN (Electronic)978-1-4939-2309-0
    ISBN (Print)978-1-4939-2308-3
    DOIs
    Publication statusPublished - 2015
    MoE publication typeD2 Article in professional manuals or guides or professional information systems or text book material

    Publication series

    SeriesMethods in Molecular Biology
    Volume1270
    ISSN1064-3745

    Keywords

    • Bimolecular fluorescence complementation
    • EYFP
    • Fluorescence microscopy
    • GFP
    • Venus
    • Protein interactions
    • Saccharomyces cerevisiae
    • Yeast
    • Mammalian

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