Binary monolayers of single-stranded oligonucleotides and blocking agent for hybridisation

Inger Vikholm-Lundin; Reetta Piskonen

doi:10.1016/j.snb.2008.04.027

Binary monolayers of single-stranded oligonucleotides and blocking agent for hybridisation

Inger Vikholm-Lundin (Corresponding Author), Reetta Piskonen

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

14 Citations (Scopus)

Abstract

The hybridisation of thiol-modified single-stranded DNA (SH-ssDNA) embedded in lipoate or hydrophilic polymer monolayers on gold have been studied through the technique of surface plasmon resonance (SPR). SH-ssDNA and the blocking agents were co-adsorbed on gold from the same solution or the surface was post-treated with the blocking agents. The assembling process is allowed to take place for only 10 min. Binding of non-complementary and complementary DNA is dependent both on the blocking agent and on the assembling method used. The lowest non-complementary binding and the highest complementary binding of DNA was obtained with a layer assembled from a binary solution of SH-ssDNA and the lipoate blocking agent.

Original language	English
Pages (from-to)	189-192
Journal	Sensors and Actuators B: Chemical
Volume	134
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2008.04.027
Publication status	Published - 2008
MoE publication type	A1 Journal article-refereed

Keywords

SH-ssDNA
Non-specific binding
Surface plasmon resonance
Hybridisation

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10.1016/j.snb.2008.04.027

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abstract = "The hybridisation of thiol-modified single-stranded DNA (SH-ssDNA) embedded in lipoate or hydrophilic polymer monolayers on gold have been studied through the technique of surface plasmon resonance (SPR). SH-ssDNA and the blocking agents were co-adsorbed on gold from the same solution or the surface was post-treated with the blocking agents. The assembling process is allowed to take place for only 10 min. Binding of non-complementary and complementary DNA is dependent both on the blocking agent and on the assembling method used. The lowest non-complementary binding and the highest complementary binding of DNA was obtained with a layer assembled from a binary solution of SH-ssDNA and the lipoate blocking agent.",

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AU - Vikholm-Lundin, Inger

AU - Piskonen, Reetta

PY - 2008

Y1 - 2008

N2 - The hybridisation of thiol-modified single-stranded DNA (SH-ssDNA) embedded in lipoate or hydrophilic polymer monolayers on gold have been studied through the technique of surface plasmon resonance (SPR). SH-ssDNA and the blocking agents were co-adsorbed on gold from the same solution or the surface was post-treated with the blocking agents. The assembling process is allowed to take place for only 10 min. Binding of non-complementary and complementary DNA is dependent both on the blocking agent and on the assembling method used. The lowest non-complementary binding and the highest complementary binding of DNA was obtained with a layer assembled from a binary solution of SH-ssDNA and the lipoate blocking agent.

AB - The hybridisation of thiol-modified single-stranded DNA (SH-ssDNA) embedded in lipoate or hydrophilic polymer monolayers on gold have been studied through the technique of surface plasmon resonance (SPR). SH-ssDNA and the blocking agents were co-adsorbed on gold from the same solution or the surface was post-treated with the blocking agents. The assembling process is allowed to take place for only 10 min. Binding of non-complementary and complementary DNA is dependent both on the blocking agent and on the assembling method used. The lowest non-complementary binding and the highest complementary binding of DNA was obtained with a layer assembled from a binary solution of SH-ssDNA and the lipoate blocking agent.

KW - SH-ssDNA

KW - Non-specific binding

KW - Surface plasmon resonance

KW - Hybridisation

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DO - 10.1016/j.snb.2008.04.027

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JO - Sensors and Actuators B: Chemical

JF - Sensors and Actuators B: Chemical

SN - 0925-4005

IS - 1

ER -

Binary monolayers of single-stranded oligonucleotides and blocking agent for hybridisation

Abstract

Keywords

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