Binary monolayers of single-stranded oligonucleotides and blocking agent for hybridisation

Inger Vikholm-Lundin (Corresponding Author), Reetta Piskonen

Research output: Contribution to journalArticleScientificpeer-review

14 Citations (Scopus)

Abstract

The hybridisation of thiol-modified single-stranded DNA (SH-ssDNA) embedded in lipoate or hydrophilic polymer monolayers on gold have been studied through the technique of surface plasmon resonance (SPR). SH-ssDNA and the blocking agents were co-adsorbed on gold from the same solution or the surface was post-treated with the blocking agents. The assembling process is allowed to take place for only 10 min. Binding of non-complementary and complementary DNA is dependent both on the blocking agent and on the assembling method used. The lowest non-complementary binding and the highest complementary binding of DNA was obtained with a layer assembled from a binary solution of SH-ssDNA and the lipoate blocking agent.
Original languageEnglish
Pages (from-to)189-192
JournalSensors and Actuators B: Chemical
Volume134
Issue number1
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

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Keywords

  • SH-ssDNA
  • Non-specific binding
  • Surface plasmon resonance
  • Hybridisation

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