Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 189 - 192 |
| Number of pages | 4 |
| Journal | Sensors and Actuators B: Chemical |
| Volume | 134 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2008 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- SH-ssDNA
- Non-specific binding
- Surface plasmon resonance
- Hybridisation
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Binary monolayers of single-stranded oligonucleotides and blocking agent for hybridisation. / Vikholm-Lundin, Inger (Corresponding Author); Piskonen, Reetta.
In: Sensors and Actuators B: Chemical, Vol. 134, No. 1, 2008, p. 189 - 192.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Binary monolayers of single-stranded oligonucleotides and blocking agent for hybridisation
AU - Vikholm-Lundin, Inger
AU - Piskonen, Reetta
PY - 2008
Y1 - 2008
N2 - The hybridisation of thiol-modified single-stranded DNA (SH-ssDNA) embedded in lipoate or hydrophilic polymer monolayers on gold have been studied through the technique of surface plasmon resonance (SPR). SH-ssDNA and the blocking agents were co-adsorbed on gold from the same solution or the surface was post-treated with the blocking agents. The assembling process is allowed to take place for only 10 min. Binding of non-complementary and complementary DNA is dependent both on the blocking agent and on the assembling method used. The lowest non-complementary binding and the highest complementary binding of DNA was obtained with a layer assembled from a binary solution of SH-ssDNA and the lipoate blocking agent.
AB - The hybridisation of thiol-modified single-stranded DNA (SH-ssDNA) embedded in lipoate or hydrophilic polymer monolayers on gold have been studied through the technique of surface plasmon resonance (SPR). SH-ssDNA and the blocking agents were co-adsorbed on gold from the same solution or the surface was post-treated with the blocking agents. The assembling process is allowed to take place for only 10 min. Binding of non-complementary and complementary DNA is dependent both on the blocking agent and on the assembling method used. The lowest non-complementary binding and the highest complementary binding of DNA was obtained with a layer assembled from a binary solution of SH-ssDNA and the lipoate blocking agent.
KW - SH-ssDNA
KW - Non-specific binding
KW - Surface plasmon resonance
KW - Hybridisation
U2 - 10.1016/j.snb.2008.04.027
DO - 10.1016/j.snb.2008.04.027
M3 - Article
VL - 134
SP - 189
EP - 192
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
SN - 0925-4005
IS - 1
ER -