Binary monolayers of single-stranded oligonucleotides and blocking agent for hybridisation

Inger Vikholm-Lundin (Corresponding Author), Reetta Piskonen

Research output: Contribution to journalArticleScientificpeer-review

13 Citations (Scopus)

Abstract

The hybridisation of thiol-modified single-stranded DNA (SH-ssDNA) embedded in lipoate or hydrophilic polymer monolayers on gold have been studied through the technique of surface plasmon resonance (SPR). SH-ssDNA and the blocking agents were co-adsorbed on gold from the same solution or the surface was post-treated with the blocking agents. The assembling process is allowed to take place for only 10 min. Binding of non-complementary and complementary DNA is dependent both on the blocking agent and on the assembling method used. The lowest non-complementary binding and the highest complementary binding of DNA was obtained with a layer assembled from a binary solution of SH-ssDNA and the lipoate blocking agent.
Original languageEnglish
Pages (from-to)189 - 192
Number of pages4
JournalSensors and Actuators B: Chemical
Volume134
Issue number1
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Fingerprint

oligonucleotides
Oligonucleotides
Monolayers
DNA
assembling
Gold
deoxyribonucleic acid
complementary DNA
gold
Single-Stranded DNA
Surface plasmon resonance
surface plasmon resonance
Sulfhydryl Compounds
thiols
Polymers
Complementary DNA
polymers

Keywords

  • SH-ssDNA
  • Non-specific binding
  • Surface plasmon resonance
  • Hybridisation

Cite this

Vikholm-Lundin, Inger ; Piskonen, Reetta. / Binary monolayers of single-stranded oligonucleotides and blocking agent for hybridisation. In: Sensors and Actuators B: Chemical. 2008 ; Vol. 134, No. 1. pp. 189 - 192.
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Binary monolayers of single-stranded oligonucleotides and blocking agent for hybridisation. / Vikholm-Lundin, Inger (Corresponding Author); Piskonen, Reetta.

In: Sensors and Actuators B: Chemical, Vol. 134, No. 1, 2008, p. 189 - 192.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Vikholm-Lundin, Inger

AU - Piskonen, Reetta

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KW - SH-ssDNA

KW - Non-specific binding

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