Abstract
The hybridisation of thiol-modified single-stranded DNA (SH-ssDNA) embedded in lipoate or hydrophilic polymer monolayers on gold have been studied through the technique of surface plasmon resonance (SPR). SH-ssDNA and the blocking agents
were co-adsorbed on gold from the same solution or the surface was
post-treated with the blocking agents. The assembling process is allowed
to take place for only 10 min. Binding of
non-complementary and complementary DNA is dependent both on the
blocking agent and on the assembling method used. The lowest
non-complementary binding and the highest complementary binding of DNA
was obtained with a layer assembled from a binary solution of SH-ssDNA
and the lipoate blocking agent.
| Original language | English |
|---|---|
| Pages (from-to) | 189-192 |
| Journal | Sensors and Actuators B: Chemical |
| Volume | 134 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2008 |
| MoE publication type | A1 Journal article-refereed |
Keywords
- SH-ssDNA
- Non-specific binding
- Surface plasmon resonance
- Hybridisation
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