TY - JOUR
T1 - Bioactivity and chemical characterization of gonads of green sea urchin Strongylocentrotus droebachiensis from Barents Sea
AU - Pozharitskaya, Olga N.
AU - Shikov, Alexander N.
AU - Laakso, Into
AU - Seppänen-Laakso, Tuulikki
AU - Makarenko, Igor E.
AU - Faustova, Natalya M.
AU - Makarova, Marina N.
AU - Makarov, Valery G.
PY - 2015
Y1 - 2015
N2 - The gonads of sea urchins have been consumed by humans
since ancient times. However, relatively little
information is available on their biological activity. In
the present study, we have consecutively extracted gonads
of green sea urchins Strongylocentrotus droebachiensis
from Barents Sea with chloroform-95% EtOH (4:1, v/v),
(fraction ESD1), 95% EtOH (ESD2), and 70% EtOH (ESD3),
characterized the fractions by GC-MS and HPLC, and
further investigated their bioactivities.
The cyclooxygenase-2 was inhibited by 62% with ESD1 and
by 39% with ESD2. All the extracts from gonads
significantly prevented the decrease in body weight of
mice having streptozotocin-nicotinamide-induced (STZ/NA)
diabetes. ESD2 significantly reduced serum glucose level
in mice after 5 days of administration. The 50%
inhibition of dipeptidyl peptidase IV was observed at a
dose of 20 µg/ml of ESD2. These results suggest that sea
urchin gonads may serve as a functional food for the
management of inflammatory diseases and diabetes.
AB - The gonads of sea urchins have been consumed by humans
since ancient times. However, relatively little
information is available on their biological activity. In
the present study, we have consecutively extracted gonads
of green sea urchins Strongylocentrotus droebachiensis
from Barents Sea with chloroform-95% EtOH (4:1, v/v),
(fraction ESD1), 95% EtOH (ESD2), and 70% EtOH (ESD3),
characterized the fractions by GC-MS and HPLC, and
further investigated their bioactivities.
The cyclooxygenase-2 was inhibited by 62% with ESD1 and
by 39% with ESD2. All the extracts from gonads
significantly prevented the decrease in body weight of
mice having streptozotocin-nicotinamide-induced (STZ/NA)
diabetes. ESD2 significantly reduced serum glucose level
in mice after 5 days of administration. The 50%
inhibition of dipeptidyl peptidase IV was observed at a
dose of 20 µg/ml of ESD2. These results suggest that sea
urchin gonads may serve as a functional food for the
management of inflammatory diseases and diabetes.
KW - Strongylocentrotus droebachiensis
KW - Gonads
KW - Cyclooxygenase
KW - Diabetes
KW - Dipeptidyl peptidase IV
KW - Echinenone
U2 - 10.1016/j.jff.2015.05.030
DO - 10.1016/j.jff.2015.05.030
M3 - Article
SN - 1756-4646
VL - 17
SP - 227
EP - 234
JO - Journal of Functional Foods
JF - Journal of Functional Foods
ER -
