Bioactivity and chemical characterization of gonads of green sea urchin Strongylocentrotus droebachiensis from Barents Sea

Olga N. Pozharitskaya, Alexander N. Shikov (Corresponding Author), Into Laakso, Tuulikki Seppänen-Laakso, Igor E. Makarenko, Natalya M. Faustova, Marina N. Makarova, Valery G. Makarov

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

The gonads of sea urchins have been consumed by humans since ancient times. However, relatively little information is available on their biological activity. In the present study, we have consecutively extracted gonads of green sea urchins Strongylocentrotus droebachiensis from Barents Sea with chloroform-95% EtOH (4:1, v/v), (fraction ESD1), 95% EtOH (ESD2), and 70% EtOH (ESD3), characterized the fractions by GC-MS and HPLC, and further investigated their bioactivities. The cyclooxygenase-2 was inhibited by 62% with ESD1 and by 39% with ESD2. All the extracts from gonads significantly prevented the decrease in body weight of mice having streptozotocin-nicotinamide-induced (STZ/NA) diabetes. ESD2 significantly reduced serum glucose level in mice after 5 days of administration. The 50% inhibition of dipeptidyl peptidase IV was observed at a dose of 20 µg/ml of ESD2. These results suggest that sea urchin gonads may serve as a functional food for the management of inflammatory diseases and diabetes.
Original languageEnglish
Pages (from-to)227-234
JournalJournal of Functional Foods
Volume17
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Fingerprint

Strongylocentrotus
Strongylocentrotus nudus
Barents Sea
Sea Urchins
Gonads
Oceans and Seas
gonads
Echinoidea
diabetes
Dipeptidyl Peptidase 4
nicotinamide
Functional Food
Niacinamide
streptozotocin
mice
prostaglandin synthase
Cyclooxygenase 2
Streptozocin
Disease Management
Chloroform

Keywords

  • Strongylocentrotus droebachiensis
  • Gonads
  • Cyclooxygenase
  • Diabetes
  • Dipeptidyl peptidase IV
  • Echinenone

Cite this

Pozharitskaya, Olga N. ; Shikov, Alexander N. ; Laakso, Into ; Seppänen-Laakso, Tuulikki ; Makarenko, Igor E. ; Faustova, Natalya M. ; Makarova, Marina N. ; Makarov, Valery G. / Bioactivity and chemical characterization of gonads of green sea urchin Strongylocentrotus droebachiensis from Barents Sea. In: Journal of Functional Foods. 2015 ; Vol. 17. pp. 227-234.
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abstract = "The gonads of sea urchins have been consumed by humans since ancient times. However, relatively little information is available on their biological activity. In the present study, we have consecutively extracted gonads of green sea urchins Strongylocentrotus droebachiensis from Barents Sea with chloroform-95{\%} EtOH (4:1, v/v), (fraction ESD1), 95{\%} EtOH (ESD2), and 70{\%} EtOH (ESD3), characterized the fractions by GC-MS and HPLC, and further investigated their bioactivities. The cyclooxygenase-2 was inhibited by 62{\%} with ESD1 and by 39{\%} with ESD2. All the extracts from gonads significantly prevented the decrease in body weight of mice having streptozotocin-nicotinamide-induced (STZ/NA) diabetes. ESD2 significantly reduced serum glucose level in mice after 5 days of administration. The 50{\%} inhibition of dipeptidyl peptidase IV was observed at a dose of 20 µg/ml of ESD2. These results suggest that sea urchin gonads may serve as a functional food for the management of inflammatory diseases and diabetes.",
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Bioactivity and chemical characterization of gonads of green sea urchin Strongylocentrotus droebachiensis from Barents Sea. / Pozharitskaya, Olga N.; Shikov, Alexander N. (Corresponding Author); Laakso, Into; Seppänen-Laakso, Tuulikki; Makarenko, Igor E.; Faustova, Natalya M.; Makarova, Marina N.; Makarov, Valery G.

In: Journal of Functional Foods, Vol. 17, 2015, p. 227-234.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

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AU - Pozharitskaya, Olga N.

AU - Shikov, Alexander N.

AU - Laakso, Into

AU - Seppänen-Laakso, Tuulikki

AU - Makarenko, Igor E.

AU - Faustova, Natalya M.

AU - Makarova, Marina N.

AU - Makarov, Valery G.

PY - 2015

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N2 - The gonads of sea urchins have been consumed by humans since ancient times. However, relatively little information is available on their biological activity. In the present study, we have consecutively extracted gonads of green sea urchins Strongylocentrotus droebachiensis from Barents Sea with chloroform-95% EtOH (4:1, v/v), (fraction ESD1), 95% EtOH (ESD2), and 70% EtOH (ESD3), characterized the fractions by GC-MS and HPLC, and further investigated their bioactivities. The cyclooxygenase-2 was inhibited by 62% with ESD1 and by 39% with ESD2. All the extracts from gonads significantly prevented the decrease in body weight of mice having streptozotocin-nicotinamide-induced (STZ/NA) diabetes. ESD2 significantly reduced serum glucose level in mice after 5 days of administration. The 50% inhibition of dipeptidyl peptidase IV was observed at a dose of 20 µg/ml of ESD2. These results suggest that sea urchin gonads may serve as a functional food for the management of inflammatory diseases and diabetes.

AB - The gonads of sea urchins have been consumed by humans since ancient times. However, relatively little information is available on their biological activity. In the present study, we have consecutively extracted gonads of green sea urchins Strongylocentrotus droebachiensis from Barents Sea with chloroform-95% EtOH (4:1, v/v), (fraction ESD1), 95% EtOH (ESD2), and 70% EtOH (ESD3), characterized the fractions by GC-MS and HPLC, and further investigated their bioactivities. The cyclooxygenase-2 was inhibited by 62% with ESD1 and by 39% with ESD2. All the extracts from gonads significantly prevented the decrease in body weight of mice having streptozotocin-nicotinamide-induced (STZ/NA) diabetes. ESD2 significantly reduced serum glucose level in mice after 5 days of administration. The 50% inhibition of dipeptidyl peptidase IV was observed at a dose of 20 µg/ml of ESD2. These results suggest that sea urchin gonads may serve as a functional food for the management of inflammatory diseases and diabetes.

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KW - Diabetes

KW - Dipeptidyl peptidase IV

KW - Echinenone

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