Biocatalytic conversion of avermectin to 4″-oxo-avermectin: Characterization of biocatalytically active bacterial strains and of cytochrome p450 monooxygenase enzymes and their genes

Volker Jungmann, István Molnár (Corresponding Author), Philip E. Hammer, D. Steven Hill, Ross Zirkle, Thomas G. Buckel, Dagmar Buckel, James M. Ligon, J. Paul Pachlatko (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

29 Citations (Scopus)

Abstract

4″-Oxo-avermectin is a key intermediate in the manufacture of the agriculturally important insecticide emamectin benzoate from the natural product avermectin. Seventeen biocatalytically active Streptomyces strains with the ability to oxidize avermectin to 4″-oxo-avermectin in a regioselective manner have been discovered in a screen of 3,334 microorganisms. The enzymes responsible for this oxidation reaction in these biocatalytically active strains were found to be cytochrome P450 monooxygenases (CYPs) and were termed Ema1 to Ema17. The genes for Ema1 to Ema17 have been cloned, sequenced, and compared to reveal a new subfamily of CYPs. Ema1 to Ema16 have been overexpressed in Escherichia coli and purified as His-tagged recombinant proteins, and their basic enzyme kinetic parameters have been determined.

Original languageEnglish
Pages (from-to)6968-6976
Number of pages9
JournalApplied and Environmental Microbiology
Volume71
Issue number11
DOIs
Publication statusPublished - Nov 2005
MoE publication typeA1 Journal article-refereed

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