Biochemical and genetic characterization of a Prevotella intermedia/nigrescens-like organism

Eija Könönen (Corresponding Author), Jaana Mättö, Marja-Liisa Väisänen-Tunkelrott, Ellen Frandsen, Ilkka Helander, Sirkka Asikainen, Sydney Finegold, Hannele Jousimies-Somer

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Abstract

Thirty-three previously non-typable faintly pigmented Gram-negative anaerobic bacterial isolates, biochemically most closely related to Prevotella intermedia and Prevotella nigrescens, were analysed for enzymic reactions, cellular fatty acid (CFA) composition, electrophoretic mobility of malate and glutamate dehydrogenases, hybridization with P. intermedia and P. nigrescens species-specific oligonucleotide probes and, for genetic heterogeneity, by arbitrarily primed PCR (AP-PCR). P. intermedia ATCC 25611T and P. nigrescens ATCC 33563T were run in parallel for comparison.
Twenty-nine isolates originated from the normal oral flora of 18 subjects (including five mother-child pairs), and four isolates from various infections. Except for a negative lipase reaction, enzymic profiles of the test isolates were similar to those of P. intermedia and P. nigrescens. Clustering of CFAs, electrophoretic mobility patterns, hybridization with DNA probes for P. intermedia and P. nigrescens, and AP-PCR band patterns of the test isolates differed from those of the type strains of P. intermedia and P. nigrescens, suggesting the existence, in humans, of a new anaerobic species of pigmented, moderately saccharolytic, indole-positive Gram-negative rods.
Original languageEnglish
Pages (from-to)39-46
JournalInternational Journal of Systematic Bacteriology
Volume48
DOIs
Publication statusPublished - 1998
MoE publication typeA1 Journal article-refereed

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Prevotella nigrescens
Prevotella intermedia
Molecular Biology
probe
organisms
flora
fatty acid
enzymatic reactions
DNA
hybridization
Gram-Positive Rods
Polymerase Chain Reaction
Glutamate Dehydrogenase
Malate Dehydrogenase
oligonucleotide probes
Genetic Heterogeneity
Oligonucleotide Probes
glutamate dehydrogenase
malate dehydrogenase
DNA probes

Cite this

Könönen, E., Mättö, J., Väisänen-Tunkelrott, M-L., Frandsen, E., Helander, I., Asikainen, S., ... Jousimies-Somer, H. (1998). Biochemical and genetic characterization of a Prevotella intermedia/nigrescens-like organism. International Journal of Systematic Bacteriology, 48, 39-46. https://doi.org/10.1099/00207713-48-1-39
Könönen, Eija ; Mättö, Jaana ; Väisänen-Tunkelrott, Marja-Liisa ; Frandsen, Ellen ; Helander, Ilkka ; Asikainen, Sirkka ; Finegold, Sydney ; Jousimies-Somer, Hannele. / Biochemical and genetic characterization of a Prevotella intermedia/nigrescens-like organism. In: International Journal of Systematic Bacteriology. 1998 ; Vol. 48. pp. 39-46.
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abstract = "Thirty-three previously non-typable faintly pigmented Gram-negative anaerobic bacterial isolates, biochemically most closely related to Prevotella intermedia and Prevotella nigrescens, were analysed for enzymic reactions, cellular fatty acid (CFA) composition, electrophoretic mobility of malate and glutamate dehydrogenases, hybridization with P. intermedia and P. nigrescens species-specific oligonucleotide probes and, for genetic heterogeneity, by arbitrarily primed PCR (AP-PCR). P. intermedia ATCC 25611T and P. nigrescens ATCC 33563T were run in parallel for comparison. Twenty-nine isolates originated from the normal oral flora of 18 subjects (including five mother-child pairs), and four isolates from various infections. Except for a negative lipase reaction, enzymic profiles of the test isolates were similar to those of P. intermedia and P. nigrescens. Clustering of CFAs, electrophoretic mobility patterns, hybridization with DNA probes for P. intermedia and P. nigrescens, and AP-PCR band patterns of the test isolates differed from those of the type strains of P. intermedia and P. nigrescens, suggesting the existence, in humans, of a new anaerobic species of pigmented, moderately saccharolytic, indole-positive Gram-negative rods.",
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Könönen, E, Mättö, J, Väisänen-Tunkelrott, M-L, Frandsen, E, Helander, I, Asikainen, S, Finegold, S & Jousimies-Somer, H 1998, 'Biochemical and genetic characterization of a Prevotella intermedia/nigrescens-like organism', International Journal of Systematic Bacteriology, vol. 48, pp. 39-46. https://doi.org/10.1099/00207713-48-1-39

Biochemical and genetic characterization of a Prevotella intermedia/nigrescens-like organism. / Könönen, Eija (Corresponding Author); Mättö, Jaana; Väisänen-Tunkelrott, Marja-Liisa; Frandsen, Ellen; Helander, Ilkka; Asikainen, Sirkka; Finegold, Sydney; Jousimies-Somer, Hannele.

In: International Journal of Systematic Bacteriology, Vol. 48, 1998, p. 39-46.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Biochemical and genetic characterization of a Prevotella intermedia/nigrescens-like organism

AU - Könönen, Eija

AU - Mättö, Jaana

AU - Väisänen-Tunkelrott, Marja-Liisa

AU - Frandsen, Ellen

AU - Helander, Ilkka

AU - Asikainen, Sirkka

AU - Finegold, Sydney

AU - Jousimies-Somer, Hannele

PY - 1998

Y1 - 1998

N2 - Thirty-three previously non-typable faintly pigmented Gram-negative anaerobic bacterial isolates, biochemically most closely related to Prevotella intermedia and Prevotella nigrescens, were analysed for enzymic reactions, cellular fatty acid (CFA) composition, electrophoretic mobility of malate and glutamate dehydrogenases, hybridization with P. intermedia and P. nigrescens species-specific oligonucleotide probes and, for genetic heterogeneity, by arbitrarily primed PCR (AP-PCR). P. intermedia ATCC 25611T and P. nigrescens ATCC 33563T were run in parallel for comparison. Twenty-nine isolates originated from the normal oral flora of 18 subjects (including five mother-child pairs), and four isolates from various infections. Except for a negative lipase reaction, enzymic profiles of the test isolates were similar to those of P. intermedia and P. nigrescens. Clustering of CFAs, electrophoretic mobility patterns, hybridization with DNA probes for P. intermedia and P. nigrescens, and AP-PCR band patterns of the test isolates differed from those of the type strains of P. intermedia and P. nigrescens, suggesting the existence, in humans, of a new anaerobic species of pigmented, moderately saccharolytic, indole-positive Gram-negative rods.

AB - Thirty-three previously non-typable faintly pigmented Gram-negative anaerobic bacterial isolates, biochemically most closely related to Prevotella intermedia and Prevotella nigrescens, were analysed for enzymic reactions, cellular fatty acid (CFA) composition, electrophoretic mobility of malate and glutamate dehydrogenases, hybridization with P. intermedia and P. nigrescens species-specific oligonucleotide probes and, for genetic heterogeneity, by arbitrarily primed PCR (AP-PCR). P. intermedia ATCC 25611T and P. nigrescens ATCC 33563T were run in parallel for comparison. Twenty-nine isolates originated from the normal oral flora of 18 subjects (including five mother-child pairs), and four isolates from various infections. Except for a negative lipase reaction, enzymic profiles of the test isolates were similar to those of P. intermedia and P. nigrescens. Clustering of CFAs, electrophoretic mobility patterns, hybridization with DNA probes for P. intermedia and P. nigrescens, and AP-PCR band patterns of the test isolates differed from those of the type strains of P. intermedia and P. nigrescens, suggesting the existence, in humans, of a new anaerobic species of pigmented, moderately saccharolytic, indole-positive Gram-negative rods.

U2 - 10.1099/00207713-48-1-39

DO - 10.1099/00207713-48-1-39

M3 - Article

VL - 48

SP - 39

EP - 46

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

ER -