Abstract
We have demonstrated that D-xylonate can be efficiently produced from D-xylose with Saccharomyces cerevisiae. 17±2 g D-xylonate l-1 at 0.23 g l-1 h-1 was produced from 23 g D-xylose l-1 (with glucose and ethanol as co-substrates) when expressing an NAD+-dependent D-xylose dehydrogenase, XylB, from Caulobacter crescentus. D-Xylonate titre and production rate were improved and xylitol production reduced, compared to strains expressing genes encoding Trichoderma reesei or pig liver NADP+-dependent D-xylose dehydrogenases. However, the production led to an intracellular accumulation of D-xylonate (up to 70 mg g-1) and xylitol (up to 18 mg g-1) and to a decreased viability of the D-xylonate producing cells. To reduce xylitol production, xylB was also expressed in a strain from which the major aldose reductase, encoded by GRE3, had been deleted. An industrial S. cerevisiae strain expressing XylB produced 43 g D-xylonate l-1 from 49 g D-xylose l-1, with an initial production rate of 0.44 g l-1 h-1.
| Original language | English |
|---|---|
| Publication status | Published - 2012 |
| Event | 34th Symposium on Biotechnology for Fuels and Chemicals - New Orleans, United States Duration: 30 Apr 2012 → 3 May 2012 Conference number: 34 |
Conference
| Conference | 34th Symposium on Biotechnology for Fuels and Chemicals |
|---|---|
| Country | United States |
| City | New Orleans |
| Period | 30/04/12 → 3/05/12 |
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Bioconversion of D-xylose to D-xylonate with Saccharomyces cerevisiae. / Nygård, Yvonne; Toivari, Mervi; Ruohonen, Laura; Penttilä, Merja; Wiebe, Marilyn.
2012. Poster session presented at 34th Symposium on Biotechnology for Fuels and Chemicals, New Orleans, United States.Research output: Contribution to conference › Conference Poster › Scientific
TY - CONF
T1 - Bioconversion of D-xylose to D-xylonate with Saccharomyces cerevisiae
AU - Nygård, Yvonne
AU - Toivari, Mervi
AU - Ruohonen, Laura
AU - Penttilä, Merja
AU - Wiebe, Marilyn
N1 - Poster Session 2 CA2: TK402 CA2: TK400
PY - 2012
Y1 - 2012
N2 - Increasing concern about climate change and fluctuation in fossil fuel prices has increased interest in development of new biomass based products. Production of organic acids using yeast is a promising approach to generate building-block chemicals from renewable carbon sources, such as lignocellulosic hydrolysates. Hydrolysed plant biomass typically contains a substantial fraction of D-xylose, which could be converted to ethanol, but might preferably be converted to other products, including D-xylonic acid. D-Xylonic acid can be used as a substitute for D-gluconic acid, e.g. to improve dispersal of concrete, as a polyamide modifier or as a precursor for 1,2,4-butanetriol.We have demonstrated that D-xylonate can be efficiently produced from D-xylose with Saccharomyces cerevisiae. 17±2 g D-xylonate l-1 at 0.23 g l-1 h-1 was produced from 23 g D-xylose l-1 (with glucose and ethanol as co-substrates) when expressing an NAD+-dependent D-xylose dehydrogenase, XylB, from Caulobacter crescentus. D-Xylonate titre and production rate were improved and xylitol production reduced, compared to strains expressing genes encoding Trichoderma reesei or pig liver NADP+-dependent D-xylose dehydrogenases. However, the production led to an intracellular accumulation of D-xylonate (up to 70 mg g-1) and xylitol (up to 18 mg g-1) and to a decreased viability of the D-xylonate producing cells. To reduce xylitol production, xylB was also expressed in a strain from which the major aldose reductase, encoded by GRE3, had been deleted. An industrial S. cerevisiae strain expressing XylB produced 43 g D-xylonate l-1 from 49 g D-xylose l-1, with an initial production rate of 0.44 g l-1 h-1.
AB - Increasing concern about climate change and fluctuation in fossil fuel prices has increased interest in development of new biomass based products. Production of organic acids using yeast is a promising approach to generate building-block chemicals from renewable carbon sources, such as lignocellulosic hydrolysates. Hydrolysed plant biomass typically contains a substantial fraction of D-xylose, which could be converted to ethanol, but might preferably be converted to other products, including D-xylonic acid. D-Xylonic acid can be used as a substitute for D-gluconic acid, e.g. to improve dispersal of concrete, as a polyamide modifier or as a precursor for 1,2,4-butanetriol.We have demonstrated that D-xylonate can be efficiently produced from D-xylose with Saccharomyces cerevisiae. 17±2 g D-xylonate l-1 at 0.23 g l-1 h-1 was produced from 23 g D-xylose l-1 (with glucose and ethanol as co-substrates) when expressing an NAD+-dependent D-xylose dehydrogenase, XylB, from Caulobacter crescentus. D-Xylonate titre and production rate were improved and xylitol production reduced, compared to strains expressing genes encoding Trichoderma reesei or pig liver NADP+-dependent D-xylose dehydrogenases. However, the production led to an intracellular accumulation of D-xylonate (up to 70 mg g-1) and xylitol (up to 18 mg g-1) and to a decreased viability of the D-xylonate producing cells. To reduce xylitol production, xylB was also expressed in a strain from which the major aldose reductase, encoded by GRE3, had been deleted. An industrial S. cerevisiae strain expressing XylB produced 43 g D-xylonate l-1 from 49 g D-xylose l-1, with an initial production rate of 0.44 g l-1 h-1.
M3 - Conference Poster
ER -