Bioconversion of D-xylose to D-xylonate with Saccharomyces cerevisiae

Yvonne Nygård, Mervi Toivari, Laura Ruohonen, Merja Penttilä, Marilyn Wiebe

Research output: Contribution to conferenceConference PosterScientific

Abstract

Increasing concern about climate change and fluctuation in fossil fuel prices has increased interest in development of new biomass based products. Production of organic acids using yeast is a promising approach to generate building-block chemicals from renewable carbon sources, such as lignocellulosic hydrolysates. Hydrolysed plant biomass typically contains a substantial fraction of D-xylose, which could be converted to ethanol, but might preferably be converted to other products, including D-xylonic acid. D-Xylonic acid can be used as a substitute for D-gluconic acid, e.g. to improve dispersal of concrete, as a polyamide modifier or as a precursor for 1,2,4-butanetriol.

We have demonstrated that D-xylonate can be efficiently produced from D-xylose with Saccharomyces cerevisiae. 17±2 g D-xylonate l-1 at 0.23 g l-1 h-1 was produced from 23 g D-xylose l-1 (with glucose and ethanol as co-substrates) when expressing an NAD+-dependent D-xylose dehydrogenase, XylB, from Caulobacter crescentus. D-Xylonate titre and production rate were improved and xylitol production reduced, compared to strains expressing genes encoding Trichoderma reesei or pig liver NADP+-dependent D-xylose dehydrogenases. However, the production led to an intracellular accumulation of D-xylonate (up to 70 mg g-1) and xylitol (up to 18 mg g-1) and to a decreased viability of the D-xylonate producing cells. To reduce xylitol production, xylB was also expressed in a strain from which the major aldose reductase, encoded by GRE3, had been deleted. An industrial S. cerevisiae strain expressing XylB produced 43 g D-xylonate l-1 from 49 g D-xylose l-1, with an initial production rate of 0.44 g l-1 h-1.
Original languageEnglish
Publication statusPublished - 2012
Event34th Symposium on Biotechnology for Fuels and Chemicals - New Orleans, United States
Duration: 30 Apr 20123 May 2012
Conference number: 34

Conference

Conference34th Symposium on Biotechnology for Fuels and Chemicals
CountryUnited States
CityNew Orleans
Period30/04/123/05/12

Fingerprint

biotransformation
xylose
Saccharomyces cerevisiae
xylitol
Caulobacter crescentus
ethanol
aldehyde reductase
gluconic acid
Trichoderma reesei
modifiers (genes)
acids
fossil fuels
hydrolysates
organic acids and salts
viability
climate change
yeasts
liver
glucose
swine

Cite this

Nygård, Y., Toivari, M., Ruohonen, L., Penttilä, M., & Wiebe, M. (2012). Bioconversion of D-xylose to D-xylonate with Saccharomyces cerevisiae. Poster session presented at 34th Symposium on Biotechnology for Fuels and Chemicals, New Orleans, United States.
Nygård, Yvonne ; Toivari, Mervi ; Ruohonen, Laura ; Penttilä, Merja ; Wiebe, Marilyn. / Bioconversion of D-xylose to D-xylonate with Saccharomyces cerevisiae. Poster session presented at 34th Symposium on Biotechnology for Fuels and Chemicals, New Orleans, United States.
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Nygård, Y, Toivari, M, Ruohonen, L, Penttilä, M & Wiebe, M 2012, 'Bioconversion of D-xylose to D-xylonate with Saccharomyces cerevisiae' 34th Symposium on Biotechnology for Fuels and Chemicals, New Orleans, United States, 30/04/12 - 3/05/12, .

Bioconversion of D-xylose to D-xylonate with Saccharomyces cerevisiae. / Nygård, Yvonne; Toivari, Mervi; Ruohonen, Laura; Penttilä, Merja; Wiebe, Marilyn.

2012. Poster session presented at 34th Symposium on Biotechnology for Fuels and Chemicals, New Orleans, United States.

Research output: Contribution to conferenceConference PosterScientific

TY - CONF

T1 - Bioconversion of D-xylose to D-xylonate with Saccharomyces cerevisiae

AU - Nygård, Yvonne

AU - Toivari, Mervi

AU - Ruohonen, Laura

AU - Penttilä, Merja

AU - Wiebe, Marilyn

N1 - Poster Session 2 CA2: TK402 CA2: TK400

PY - 2012

Y1 - 2012

N2 - Increasing concern about climate change and fluctuation in fossil fuel prices has increased interest in development of new biomass based products. Production of organic acids using yeast is a promising approach to generate building-block chemicals from renewable carbon sources, such as lignocellulosic hydrolysates. Hydrolysed plant biomass typically contains a substantial fraction of D-xylose, which could be converted to ethanol, but might preferably be converted to other products, including D-xylonic acid. D-Xylonic acid can be used as a substitute for D-gluconic acid, e.g. to improve dispersal of concrete, as a polyamide modifier or as a precursor for 1,2,4-butanetriol.We have demonstrated that D-xylonate can be efficiently produced from D-xylose with Saccharomyces cerevisiae. 17±2 g D-xylonate l-1 at 0.23 g l-1 h-1 was produced from 23 g D-xylose l-1 (with glucose and ethanol as co-substrates) when expressing an NAD+-dependent D-xylose dehydrogenase, XylB, from Caulobacter crescentus. D-Xylonate titre and production rate were improved and xylitol production reduced, compared to strains expressing genes encoding Trichoderma reesei or pig liver NADP+-dependent D-xylose dehydrogenases. However, the production led to an intracellular accumulation of D-xylonate (up to 70 mg g-1) and xylitol (up to 18 mg g-1) and to a decreased viability of the D-xylonate producing cells. To reduce xylitol production, xylB was also expressed in a strain from which the major aldose reductase, encoded by GRE3, had been deleted. An industrial S. cerevisiae strain expressing XylB produced 43 g D-xylonate l-1 from 49 g D-xylose l-1, with an initial production rate of 0.44 g l-1 h-1.

AB - Increasing concern about climate change and fluctuation in fossil fuel prices has increased interest in development of new biomass based products. Production of organic acids using yeast is a promising approach to generate building-block chemicals from renewable carbon sources, such as lignocellulosic hydrolysates. Hydrolysed plant biomass typically contains a substantial fraction of D-xylose, which could be converted to ethanol, but might preferably be converted to other products, including D-xylonic acid. D-Xylonic acid can be used as a substitute for D-gluconic acid, e.g. to improve dispersal of concrete, as a polyamide modifier or as a precursor for 1,2,4-butanetriol.We have demonstrated that D-xylonate can be efficiently produced from D-xylose with Saccharomyces cerevisiae. 17±2 g D-xylonate l-1 at 0.23 g l-1 h-1 was produced from 23 g D-xylose l-1 (with glucose and ethanol as co-substrates) when expressing an NAD+-dependent D-xylose dehydrogenase, XylB, from Caulobacter crescentus. D-Xylonate titre and production rate were improved and xylitol production reduced, compared to strains expressing genes encoding Trichoderma reesei or pig liver NADP+-dependent D-xylose dehydrogenases. However, the production led to an intracellular accumulation of D-xylonate (up to 70 mg g-1) and xylitol (up to 18 mg g-1) and to a decreased viability of the D-xylonate producing cells. To reduce xylitol production, xylB was also expressed in a strain from which the major aldose reductase, encoded by GRE3, had been deleted. An industrial S. cerevisiae strain expressing XylB produced 43 g D-xylonate l-1 from 49 g D-xylose l-1, with an initial production rate of 0.44 g l-1 h-1.

M3 - Conference Poster

ER -

Nygård Y, Toivari M, Ruohonen L, Penttilä M, Wiebe M. Bioconversion of D-xylose to D-xylonate with Saccharomyces cerevisiae. 2012. Poster session presented at 34th Symposium on Biotechnology for Fuels and Chemicals, New Orleans, United States.