Bioconversion of d-xylose to d-xylonate with Kluyveromyces lactis

Yvonne Nygård, Mervi H. Toivari, Merja Penttilä, Laura Ruohonen, Marilyn G. Wiebe

Research output: Contribution to journalArticleScientificpeer-review

36 Citations (Scopus)

Abstract

d-Xylonate was produced from d-xylose using Kluyveromyces lactis strains which expressed the gene for NADP +-dependent d-xylose dehydrogenase from Trichoderma reesei (xyd1). Up to 19±2g d-xylonatel -1 was produced when K. lactis expressing xyd1 was grown on 10.5gd-galactosel -1 and 40g d-xylosel -1. Intracellular accumulation of d-xylonate (up to ~70mg [gbiomass] -1) was observed.d-Xylose was metabolised to d-xylonate, xylitol and biomass. Oxygen could be reduced to 6mmolO 2l -1h -1 without loss in titre or production rate, but metabolism of d-xylose and xylitol were more efficient when 12mmolO 2l -1h -1 were provided.d-Xylose uptake was not affected by deletion of either the d-xylose reductase (XYL1) or a putative xylitol dehydrogenase encoding gene (XYL2) in xyd1 expressing strains. K. lactis xyd1Δ XYL1 did not produce extracellular xylitol and produced more d-xylonate than the xyd1 strain containing the endogenous XYL1. K. lactis xyd1Δ. XYL2 produced high concentrations of xylitol and significantly less d-xylonate than the xyd1 strain with the endogenous XYL2.

Original languageEnglish
Pages (from-to)383-391
JournalMetabolic Engineering
Volume13
Issue number4
DOIs
Publication statusPublished - 1 Jul 2011
MoE publication typeA1 Journal article-refereed

Keywords

  • Aeration
  • D-xylonic acid
  • D-xylose
  • D-xylose reductase
  • K. lactis
  • Xylitol dehydrogenase

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