Abstract
d-Xylonate was produced from d-xylose using Kluyveromyces lactis strains which expressed the gene for NADP +-dependent d-xylose dehydrogenase from Trichoderma reesei (xyd1). Up to 19±2g d-xylonatel -1 was produced when K. lactis expressing xyd1 was grown on 10.5gd-galactosel -1 and 40g d-xylosel -1. Intracellular accumulation of d-xylonate (up to ~70mg [gbiomass] -1) was observed.d-Xylose was metabolised to d-xylonate, xylitol and biomass. Oxygen could be reduced to 6mmolO 2l -1h -1 without loss in titre or production rate, but metabolism of d-xylose and xylitol were more efficient when 12mmolO 2l -1h -1 were provided.d-Xylose uptake was not affected by deletion of either the d-xylose reductase (XYL1) or a putative xylitol dehydrogenase encoding gene (XYL2) in xyd1 expressing strains. K. lactis xyd1Δ XYL1 did not produce extracellular xylitol and produced more d-xylonate than the xyd1 strain containing the endogenous XYL1. K. lactis xyd1Δ. XYL2 produced high concentrations of xylitol and significantly less d-xylonate than the xyd1 strain with the endogenous XYL2.
| Original language | English |
|---|---|
| Pages (from-to) | 383-391 |
| Journal | Metabolic Engineering |
| Volume | 13 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 1 Jul 2011 |
| MoE publication type | A1 Journal article-refereed |
Fingerprint
Keywords
- Aeration
- D-xylonic acid
- D-xylose
- D-xylose reductase
- K. lactis
- Xylitol dehydrogenase
Cite this
}
Bioconversion of d-xylose to d-xylonate with Kluyveromyces lactis. / Nygård, Yvonne; Toivari, Mervi H.; Penttilä, Merja; Ruohonen, Laura; Wiebe, Marilyn G.
In: Metabolic Engineering, Vol. 13, No. 4, 01.07.2011, p. 383-391.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Bioconversion of d-xylose to d-xylonate with Kluyveromyces lactis
AU - Nygård, Yvonne
AU - Toivari, Mervi H.
AU - Penttilä, Merja
AU - Ruohonen, Laura
AU - Wiebe, Marilyn G.
N1 - CA2: TK402 CA2: TK400 SDA: BIC ISI: BIOTECHNOLOGY & APPLIED MICROBIOLOGY
PY - 2011/7/1
Y1 - 2011/7/1
N2 - d-Xylonate was produced from d-xylose using Kluyveromyces lactis strains which expressed the gene for NADP +-dependent d-xylose dehydrogenase from Trichoderma reesei (xyd1). Up to 19±2g d-xylonatel -1 was produced when K. lactis expressing xyd1 was grown on 10.5gd-galactosel -1 and 40g d-xylosel -1. Intracellular accumulation of d-xylonate (up to ~70mg [gbiomass] -1) was observed.d-Xylose was metabolised to d-xylonate, xylitol and biomass. Oxygen could be reduced to 6mmolO 2l -1h -1 without loss in titre or production rate, but metabolism of d-xylose and xylitol were more efficient when 12mmolO 2l -1h -1 were provided.d-Xylose uptake was not affected by deletion of either the d-xylose reductase (XYL1) or a putative xylitol dehydrogenase encoding gene (XYL2) in xyd1 expressing strains. K. lactis xyd1Δ XYL1 did not produce extracellular xylitol and produced more d-xylonate than the xyd1 strain containing the endogenous XYL1. K. lactis xyd1Δ. XYL2 produced high concentrations of xylitol and significantly less d-xylonate than the xyd1 strain with the endogenous XYL2.
AB - d-Xylonate was produced from d-xylose using Kluyveromyces lactis strains which expressed the gene for NADP +-dependent d-xylose dehydrogenase from Trichoderma reesei (xyd1). Up to 19±2g d-xylonatel -1 was produced when K. lactis expressing xyd1 was grown on 10.5gd-galactosel -1 and 40g d-xylosel -1. Intracellular accumulation of d-xylonate (up to ~70mg [gbiomass] -1) was observed.d-Xylose was metabolised to d-xylonate, xylitol and biomass. Oxygen could be reduced to 6mmolO 2l -1h -1 without loss in titre or production rate, but metabolism of d-xylose and xylitol were more efficient when 12mmolO 2l -1h -1 were provided.d-Xylose uptake was not affected by deletion of either the d-xylose reductase (XYL1) or a putative xylitol dehydrogenase encoding gene (XYL2) in xyd1 expressing strains. K. lactis xyd1Δ XYL1 did not produce extracellular xylitol and produced more d-xylonate than the xyd1 strain containing the endogenous XYL1. K. lactis xyd1Δ. XYL2 produced high concentrations of xylitol and significantly less d-xylonate than the xyd1 strain with the endogenous XYL2.
KW - Aeration
KW - D-xylonic acid
KW - D-xylose
KW - D-xylose reductase
KW - K. lactis
KW - Xylitol dehydrogenase
UR - http://www.scopus.com/inward/record.url?scp=79958722680&partnerID=8YFLogxK
U2 - 10.1016/j.ymben.2011.04.001
DO - 10.1016/j.ymben.2011.04.001
M3 - Article
C2 - 21515401
AN - SCOPUS:79958722680
VL - 13
SP - 383
EP - 391
JO - Metabolic Engineering
JF - Metabolic Engineering
SN - 1096-7176
IS - 4
ER -