Bioconversion of d-xylose to d-xylonate with Kluyveromyces lactis

Yvonne Nygård, Mervi H. Toivari, Merja Penttilä, Laura Ruohonen, Marilyn G. Wiebe

Research output: Contribution to journalArticleScientificpeer-review

30 Citations (Scopus)

Abstract

d-Xylonate was produced from d-xylose using Kluyveromyces lactis strains which expressed the gene for NADP +-dependent d-xylose dehydrogenase from Trichoderma reesei (xyd1). Up to 19±2g d-xylonatel -1 was produced when K. lactis expressing xyd1 was grown on 10.5gd-galactosel -1 and 40g d-xylosel -1. Intracellular accumulation of d-xylonate (up to ~70mg [gbiomass] -1) was observed.d-Xylose was metabolised to d-xylonate, xylitol and biomass. Oxygen could be reduced to 6mmolO 2l -1h -1 without loss in titre or production rate, but metabolism of d-xylose and xylitol were more efficient when 12mmolO 2l -1h -1 were provided.d-Xylose uptake was not affected by deletion of either the d-xylose reductase (XYL1) or a putative xylitol dehydrogenase encoding gene (XYL2) in xyd1 expressing strains. K. lactis xyd1Δ XYL1 did not produce extracellular xylitol and produced more d-xylonate than the xyd1 strain containing the endogenous XYL1. K. lactis xyd1Δ. XYL2 produced high concentrations of xylitol and significantly less d-xylonate than the xyd1 strain with the endogenous XYL2.

Original languageEnglish
Pages (from-to)383-391
JournalMetabolic Engineering
Volume13
Issue number4
DOIs
Publication statusPublished - 1 Jul 2011
MoE publication typeA1 Journal article-refereed

Fingerprint

Kluyveromyces
Bioconversion
Xylose
Xylitol
D-Xylulose Reductase
Aldehyde Reductase
Trichoderma
Gene encoding
NADP
Metabolism
Biomass
Genes
Oxidoreductases
Oxygen

Keywords

  • Aeration
  • D-xylonic acid
  • D-xylose
  • D-xylose reductase
  • K. lactis
  • Xylitol dehydrogenase

Cite this

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title = "Bioconversion of d-xylose to d-xylonate with Kluyveromyces lactis",
abstract = "d-Xylonate was produced from d-xylose using Kluyveromyces lactis strains which expressed the gene for NADP +-dependent d-xylose dehydrogenase from Trichoderma reesei (xyd1). Up to 19±2g d-xylonatel -1 was produced when K. lactis expressing xyd1 was grown on 10.5gd-galactosel -1 and 40g d-xylosel -1. Intracellular accumulation of d-xylonate (up to ~70mg [gbiomass] -1) was observed.d-Xylose was metabolised to d-xylonate, xylitol and biomass. Oxygen could be reduced to 6mmolO 2l -1h -1 without loss in titre or production rate, but metabolism of d-xylose and xylitol were more efficient when 12mmolO 2l -1h -1 were provided.d-Xylose uptake was not affected by deletion of either the d-xylose reductase (XYL1) or a putative xylitol dehydrogenase encoding gene (XYL2) in xyd1 expressing strains. K. lactis xyd1Δ XYL1 did not produce extracellular xylitol and produced more d-xylonate than the xyd1 strain containing the endogenous XYL1. K. lactis xyd1Δ. XYL2 produced high concentrations of xylitol and significantly less d-xylonate than the xyd1 strain with the endogenous XYL2.",
keywords = "Aeration, D-xylonic acid, D-xylose, D-xylose reductase, K. lactis, Xylitol dehydrogenase",
author = "Yvonne Nyg{\aa}rd and Toivari, {Mervi H.} and Merja Penttil{\"a} and Laura Ruohonen and Wiebe, {Marilyn G.}",
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Bioconversion of d-xylose to d-xylonate with Kluyveromyces lactis. / Nygård, Yvonne; Toivari, Mervi H.; Penttilä, Merja; Ruohonen, Laura; Wiebe, Marilyn G.

In: Metabolic Engineering, Vol. 13, No. 4, 01.07.2011, p. 383-391.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Bioconversion of d-xylose to d-xylonate with Kluyveromyces lactis

AU - Nygård, Yvonne

AU - Toivari, Mervi H.

AU - Penttilä, Merja

AU - Ruohonen, Laura

AU - Wiebe, Marilyn G.

N1 - CA2: TK402 CA2: TK400 SDA: BIC ISI: BIOTECHNOLOGY & APPLIED MICROBIOLOGY

PY - 2011/7/1

Y1 - 2011/7/1

N2 - d-Xylonate was produced from d-xylose using Kluyveromyces lactis strains which expressed the gene for NADP +-dependent d-xylose dehydrogenase from Trichoderma reesei (xyd1). Up to 19±2g d-xylonatel -1 was produced when K. lactis expressing xyd1 was grown on 10.5gd-galactosel -1 and 40g d-xylosel -1. Intracellular accumulation of d-xylonate (up to ~70mg [gbiomass] -1) was observed.d-Xylose was metabolised to d-xylonate, xylitol and biomass. Oxygen could be reduced to 6mmolO 2l -1h -1 without loss in titre or production rate, but metabolism of d-xylose and xylitol were more efficient when 12mmolO 2l -1h -1 were provided.d-Xylose uptake was not affected by deletion of either the d-xylose reductase (XYL1) or a putative xylitol dehydrogenase encoding gene (XYL2) in xyd1 expressing strains. K. lactis xyd1Δ XYL1 did not produce extracellular xylitol and produced more d-xylonate than the xyd1 strain containing the endogenous XYL1. K. lactis xyd1Δ. XYL2 produced high concentrations of xylitol and significantly less d-xylonate than the xyd1 strain with the endogenous XYL2.

AB - d-Xylonate was produced from d-xylose using Kluyveromyces lactis strains which expressed the gene for NADP +-dependent d-xylose dehydrogenase from Trichoderma reesei (xyd1). Up to 19±2g d-xylonatel -1 was produced when K. lactis expressing xyd1 was grown on 10.5gd-galactosel -1 and 40g d-xylosel -1. Intracellular accumulation of d-xylonate (up to ~70mg [gbiomass] -1) was observed.d-Xylose was metabolised to d-xylonate, xylitol and biomass. Oxygen could be reduced to 6mmolO 2l -1h -1 without loss in titre or production rate, but metabolism of d-xylose and xylitol were more efficient when 12mmolO 2l -1h -1 were provided.d-Xylose uptake was not affected by deletion of either the d-xylose reductase (XYL1) or a putative xylitol dehydrogenase encoding gene (XYL2) in xyd1 expressing strains. K. lactis xyd1Δ XYL1 did not produce extracellular xylitol and produced more d-xylonate than the xyd1 strain containing the endogenous XYL1. K. lactis xyd1Δ. XYL2 produced high concentrations of xylitol and significantly less d-xylonate than the xyd1 strain with the endogenous XYL2.

KW - Aeration

KW - D-xylonic acid

KW - D-xylose

KW - D-xylose reductase

KW - K. lactis

KW - Xylitol dehydrogenase

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U2 - 10.1016/j.ymben.2011.04.001

DO - 10.1016/j.ymben.2011.04.001

M3 - Article

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VL - 13

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EP - 391

JO - Metabolic Engineering

JF - Metabolic Engineering

SN - 1096-7176

IS - 4

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