Abstract
Bioconversion, i.e., the use of biological systems to
perform chemical changes in synthetic or natural
compounds in mild conditions, is an attractive tool for
the production of novel active or high-value compounds.
Plant cells exhibit a vast biochemical potential, being
able to transform a range of substances, including
pharmaceutical ingredients and industrial by-products,
via enzymatic processes. The use of plant cell cultures
offers possibilities for contained and optimized
production processes which can be applied in industrial
scale. Raspberry ketone [4-(4-hydroxyphenyl)butan-2-one]
is among the most interesting natural flavor compounds,
due to its high demand and significant market value. The
biosynthesis of this industrially relevant flavor
compound is relatively well characterized, involving the
condensation of 4-coumaryl-CoA and malonyl-CoA by Type
III polyketide synthase to form a diketide, and the
subsequent reduction catalyzed by an NADPH-dependent
reductase. Raspberry ketone has been successfully
produced by bioconversion using different hosts and
precursors to establish more efficient and economical
processes. In this work, we studied the effect of
overexpressed RiZS1 in tobacco on precursor bioconversion
to raspberry ketone. In addition, various wild type plant
cell cultures were studied for their capacity to carry
out the bioconversion to raspberry ketone using either
4-hydroxybenzalacetone or betuligenol as a substrate.
Apparently plant cells possess rather widely distributed
reductase activity capable of performing the
bioconversion to raspberry ketone using cheap and readily
available precursors.
Original language | English |
---|---|
Article number | 1035 |
Pages (from-to) | 1-9 |
Number of pages | 9 |
Journal | Frontiers in Plant Science |
Volume | 6 |
DOIs | |
Publication status | Published - 24 Nov 2015 |
MoE publication type | A1 Journal article-refereed |
Keywords
- betuligenol
- bioconversion
- 4-hydroxybenzalacetone
- plant cell culture
- raspberry ketone