Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types

Yana Tarakanchikova; Jamal Alzubi; Valentina Pennucci; Marie Follo; Boris Kochergin; Albert Muslimov; Ilya Skovorodkin; Seppo Vainio; Maria N. Antipina; Vsevolod Atkin; Alexey Popov; Igor Meglinski; Toni Cathomen; Tatjana I. Cornu; Dmitry A. Gorin; Gleb B. Sukhorukov; Irina Nazarenko

doi:10.1002/smll.201904880

Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types

Yana Tarakanchikova^*, Jamal Alzubi, Valentina Pennucci, Marie Follo, Boris Kochergin, Albert Muslimov, Ilya Skovorodkin, Seppo Vainio, Maria N. Antipina, Vsevolod Atkin, Alexey Popov, Igor Meglinski, Toni Cathomen, Tatjana I. Cornu, Dmitry A. Gorin, Gleb B. Sukhorukov, Irina Nazarenko

^*Corresponding author for this work

Not published at VTT

Research output: Contribution to journal › Article › Scientific › peer-review

30 Citations (Scopus)

Abstract

Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co-transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100% efficiency is reached using only 2.5 × 10⁻⁴ pmol of siRNA, and 1 × 10⁻³ nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo.

Original language	English
Article number	1904880
Journal	Small
Volume	16
Issue number	3
DOIs	https://doi.org/10.1002/smll.201904880
Publication status	Published - 1 Jan 2020
MoE publication type	A1 Journal article-refereed

Funding

The work was supported by BMBF IB-GUS/RUS 01DJ15026 and ERA-RusPlus/ID110 Exodiagnos/BMWi ZIM collaboration project KF2979902CR4 to IN; by Government of the Russian Federation (Grant No 14.Z50.31.0004 to support scientific research projects implemented under the supervision of leading scientists at Russian institutions and Russian institutions of higher education) to G.B.S. and D.A.G., Y.T., V.A. The authors acknowledge e-cost Actions COST-BM2012-MEHAD and COST-BM1401-Raman4Clinics.

Keywords

biomimetics
drug delivery
extracellular vesicles
gene delivery
microcapsules
nanocapsules

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10.1002/smll.201904880

Cite this

Tarakanchikova, Y., Alzubi, J., Pennucci, V., Follo, M., Kochergin, B., Muslimov, A., Skovorodkin, I., Vainio, S., Antipina, M. N., Atkin, V., Popov, A., Meglinski, I., Cathomen, T., Cornu, T. I., Gorin, D. A., Sukhorukov, G. B., & Nazarenko, I. (2020). Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types. Small, 16(3), Article 1904880. https://doi.org/10.1002/smll.201904880

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abstract = "Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co-transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100\% efficiency is reached using only 2.5 × 10−4 pmol of siRNA, and 1 × 10−3 nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo.",

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author = "Yana Tarakanchikova and Jamal Alzubi and Valentina Pennucci and Marie Follo and Boris Kochergin and Albert Muslimov and Ilya Skovorodkin and Seppo Vainio and Antipina, \{Maria N.\} and Vsevolod Atkin and Alexey Popov and Igor Meglinski and Toni Cathomen and Cornu, \{Tatjana I.\} and Gorin, \{Dmitry A.\} and Sukhorukov, \{Gleb B.\} and Irina Nazarenko",

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Tarakanchikova, Y, Alzubi, J, Pennucci, V, Follo, M, Kochergin, B, Muslimov, A, Skovorodkin, I, Vainio, S, Antipina, MN, Atkin, V, Popov, A, Meglinski, I, Cathomen, T, Cornu, TI, Gorin, DA, Sukhorukov, GB & Nazarenko, I 2020, 'Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types', Small, vol. 16, no. 3, 1904880. https://doi.org/10.1002/smll.201904880

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AU - Tarakanchikova, Yana

AU - Alzubi, Jamal

AU - Pennucci, Valentina

AU - Follo, Marie

AU - Kochergin, Boris

AU - Muslimov, Albert

AU - Skovorodkin, Ilya

AU - Vainio, Seppo

AU - Antipina, Maria N.

AU - Atkin, Vsevolod

AU - Popov, Alexey

AU - Meglinski, Igor

AU - Cathomen, Toni

AU - Cornu, Tatjana I.

AU - Gorin, Dmitry A.

AU - Sukhorukov, Gleb B.

AU - Nazarenko, Irina

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co-transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100% efficiency is reached using only 2.5 × 10−4 pmol of siRNA, and 1 × 10−3 nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo.

AB - Efficient delivery of genetic material to primary cells remains challenging. Here, efficient transfer of genetic material is presented using synthetic biodegradable nanocarriers, resembling extracellular vesicles in their biomechanical properties. This is based on two main technological achievements: generation of soft biodegradable polyelectrolyte capsules in nanosize and efficient application of the nanocapsules for co-transfer of different RNAs to tumor cell lines and primary cells, including hematopoietic progenitor cells and primary T cells. Near to 100% efficiency is reached using only 2.5 × 10−4 pmol of siRNA, and 1 × 10−3 nmol of mRNA per cell, which is several magnitude orders below the amounts reported for any of methods published so far. The data show that biodegradable nanocapsules represent a universal and highly efficient biomimetic platform for the transfer of genetic material with the utmost potential to revolutionize gene transfer technology in vitro and in vivo.

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Biodegradable Nanocarriers Resembling Extracellular Vesicles Deliver Genetic Material with the Highest Efficiency to Various Cell Types

Abstract

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Keywords

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