Biodegradation of radiolabelled synthetic lignin (14C-DHP) and mechanical pulp in a compost environment

Marja Tuomela, Annele Hatakka, Sanni Raiskila, Minna Vikman, Merja Itävaara

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Abstract

Mineralization of radioactive synthetic lignin (14C-DHP) was studied in a compost environment at 35, 50 and 58 degrees C. Compost samples were successively extracted with water, dioxane and alkali, and the molecular weight distribution of some extracts was determined by gel permeation chromatography (GPC). Biodegradation of lignin-containing spruce groundwood (SGW) and pine sawdust was concurrently determined in controlled composting tests by measuring evolved CO2. The temperatures were the same as in the 14C-DHP mineralization experiment and bleached kraft paper, with a lignin content of 0.2%, was used as a reference. The mineralization of 14C-DHP was relatively high (23-24%) at 35 degrees C and 50 degrees C, although the mixed population of compost obviously lacks the most effective lignin degraders. At 58 degrees C the mineralization of 14C-DHP, as well as the biodegradation of SGW and sawdust, was very low, indicating that the lignin-degrading organisms of compost were inactivated at this temperature. SGW was poorly biodegradable (<40%) in controlled composting tests compared with kraft paper (77-86%) at all temperatures, which means that lignin inhibits the degradation of carbohydrates. During the incubation, water-soluble degradation products, mainly monomers and dimers, and the original 14C-DHP were either mineralized or bound to humic substances. A substantial fraction of 14C-DHP was incorporated into humin or other insolubles.
Original languageEnglish
Pages (from-to)492-499
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume55
Issue number4
DOIs
Publication statusPublished - 2001
MoE publication typeA1 Journal article-refereed

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Lignin
Soil
Temperature
Humic Substances
Water
Alkalies
Gel Chromatography
Molecular Weight
Carbohydrates
Population

Cite this

Tuomela, Marja ; Hatakka, Annele ; Raiskila, Sanni ; Vikman, Minna ; Itävaara, Merja. / Biodegradation of radiolabelled synthetic lignin (14C-DHP) and mechanical pulp in a compost environment. In: Applied Microbiology and Biotechnology. 2001 ; Vol. 55, No. 4. pp. 492-499.
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abstract = "Mineralization of radioactive synthetic lignin (14C-DHP) was studied in a compost environment at 35, 50 and 58 degrees C. Compost samples were successively extracted with water, dioxane and alkali, and the molecular weight distribution of some extracts was determined by gel permeation chromatography (GPC). Biodegradation of lignin-containing spruce groundwood (SGW) and pine sawdust was concurrently determined in controlled composting tests by measuring evolved CO2. The temperatures were the same as in the 14C-DHP mineralization experiment and bleached kraft paper, with a lignin content of 0.2{\%}, was used as a reference. The mineralization of 14C-DHP was relatively high (23-24{\%}) at 35 degrees C and 50 degrees C, although the mixed population of compost obviously lacks the most effective lignin degraders. At 58 degrees C the mineralization of 14C-DHP, as well as the biodegradation of SGW and sawdust, was very low, indicating that the lignin-degrading organisms of compost were inactivated at this temperature. SGW was poorly biodegradable (<40{\%}) in controlled composting tests compared with kraft paper (77-86{\%}) at all temperatures, which means that lignin inhibits the degradation of carbohydrates. During the incubation, water-soluble degradation products, mainly monomers and dimers, and the original 14C-DHP were either mineralized or bound to humic substances. A substantial fraction of 14C-DHP was incorporated into humin or other insolubles.",
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Biodegradation of radiolabelled synthetic lignin (14C-DHP) and mechanical pulp in a compost environment. / Tuomela, Marja; Hatakka, Annele; Raiskila, Sanni; Vikman, Minna; Itävaara, Merja.

In: Applied Microbiology and Biotechnology, Vol. 55, No. 4, 2001, p. 492-499.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Biodegradation of radiolabelled synthetic lignin (14C-DHP) and mechanical pulp in a compost environment

AU - Tuomela, Marja

AU - Hatakka, Annele

AU - Raiskila, Sanni

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AU - Itävaara, Merja

PY - 2001

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AB - Mineralization of radioactive synthetic lignin (14C-DHP) was studied in a compost environment at 35, 50 and 58 degrees C. Compost samples were successively extracted with water, dioxane and alkali, and the molecular weight distribution of some extracts was determined by gel permeation chromatography (GPC). Biodegradation of lignin-containing spruce groundwood (SGW) and pine sawdust was concurrently determined in controlled composting tests by measuring evolved CO2. The temperatures were the same as in the 14C-DHP mineralization experiment and bleached kraft paper, with a lignin content of 0.2%, was used as a reference. The mineralization of 14C-DHP was relatively high (23-24%) at 35 degrees C and 50 degrees C, although the mixed population of compost obviously lacks the most effective lignin degraders. At 58 degrees C the mineralization of 14C-DHP, as well as the biodegradation of SGW and sawdust, was very low, indicating that the lignin-degrading organisms of compost were inactivated at this temperature. SGW was poorly biodegradable (<40%) in controlled composting tests compared with kraft paper (77-86%) at all temperatures, which means that lignin inhibits the degradation of carbohydrates. During the incubation, water-soluble degradation products, mainly monomers and dimers, and the original 14C-DHP were either mineralized or bound to humic substances. A substantial fraction of 14C-DHP was incorporated into humin or other insolubles.

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