Bioleaching phosphorus from fluorapatites with acidophilic bacteria

Research output: Contribution to journalArticleScientificpeer-review

15 Citations (Scopus)

Abstract

Currently known high grade easily-acquirable phosphate rock reserves are depleting. The objective of this study was to evaluate the potential application of bioleaching technology to the recovery of phosphorus from different phosphate ore materials, and isolate indigenous phosphorus-solubilising bacterial strains from them. In the bacterial enrichment and isolation experiments no growth was detected in the medium designed for acidophiles. Heterotrophic phosphorus solubilising isolates were enriched from the fluorapatite concentrate, and all four isolates were most closely related to Burkholderia fungorum. Bioleaching of phosphorus from low grade fluorapatite ore containing 8.2% P2O5 and from fluorapatite concentrate containing 29.8% P2O5 was carried out in shake flasks. Supplemental elemental sulphur was added as an energy source for acid generation. Mixed and pure acidophilic bacterial cultures consisting of iron- and/or sulphur-oxidizing bacteria Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans were used in the experiments. These acidophiles are commonly used in bioleaching of sulphide minerals, but their application to phosphorus solubilisation has until now been limited. Phosphorus leaching was shown to be a pH-dependent phenomenon. Phosphorus bioleaching yields of up to 97% and 28% were obtained in 21 d for low grade fluorapatite ore and concentrate, respectively, in solutions with a 1% solid content. With adaptation, the bacterial mixture was acclimated to suspensions with a 10-30% solid content. These results indicate a potential for the application of bioleaching to phosphorus extraction of low grade materials.
Original languageEnglish
Pages (from-to)269-275
JournalHydrometallurgy
Volume150
DOIs
Publication statusPublished - 2014
MoE publication typeA1 Journal article-refereed

Fingerprint

Bioleaching
Phosphorus
Bacteria
phosphorus pentoxide
Ores
Sulfur
Phosphates
Sulfide minerals
fluorapatite
Leaching
Suspensions
Iron
Experiments
Rocks
Recovery
Acids

Keywords

  • phosphorus
  • apatite
  • bioleaching
  • low-grade ores
  • acidithiobacillus

Cite this

@article{8e51c0cc05a04fc49ad0673d6d60b9bf,
title = "Bioleaching phosphorus from fluorapatites with acidophilic bacteria",
abstract = "Currently known high grade easily-acquirable phosphate rock reserves are depleting. The objective of this study was to evaluate the potential application of bioleaching technology to the recovery of phosphorus from different phosphate ore materials, and isolate indigenous phosphorus-solubilising bacterial strains from them. In the bacterial enrichment and isolation experiments no growth was detected in the medium designed for acidophiles. Heterotrophic phosphorus solubilising isolates were enriched from the fluorapatite concentrate, and all four isolates were most closely related to Burkholderia fungorum. Bioleaching of phosphorus from low grade fluorapatite ore containing 8.2{\%} P2O5 and from fluorapatite concentrate containing 29.8{\%} P2O5 was carried out in shake flasks. Supplemental elemental sulphur was added as an energy source for acid generation. Mixed and pure acidophilic bacterial cultures consisting of iron- and/or sulphur-oxidizing bacteria Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans were used in the experiments. These acidophiles are commonly used in bioleaching of sulphide minerals, but their application to phosphorus solubilisation has until now been limited. Phosphorus leaching was shown to be a pH-dependent phenomenon. Phosphorus bioleaching yields of up to 97{\%} and 28{\%} were obtained in 21 d for low grade fluorapatite ore and concentrate, respectively, in solutions with a 1{\%} solid content. With adaptation, the bacterial mixture was acclimated to suspensions with a 10-30{\%} solid content. These results indicate a potential for the application of bioleaching to phosphorus extraction of low grade materials.",
keywords = "phosphorus, apatite, bioleaching, low-grade ores, acidithiobacillus",
author = "Outi Priha and Tuija Sarlin and Peter Blomberg and L. Wendling and Jarno M{\"a}kinen and Mona Arnold and P{\"a}ivi Kinnunen",
note = "Project code: 81105",
year = "2014",
doi = "10.1016/j.hydromet.2014.08.002",
language = "English",
volume = "150",
pages = "269--275",
journal = "Hydrometallurgy",
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}

Bioleaching phosphorus from fluorapatites with acidophilic bacteria. / Priha, Outi; Sarlin, Tuija; Blomberg, Peter; Wendling, L.; Mäkinen, Jarno; Arnold, Mona; Kinnunen, Päivi.

In: Hydrometallurgy, Vol. 150, 2014, p. 269-275.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Bioleaching phosphorus from fluorapatites with acidophilic bacteria

AU - Priha, Outi

AU - Sarlin, Tuija

AU - Blomberg, Peter

AU - Wendling, L.

AU - Mäkinen, Jarno

AU - Arnold, Mona

AU - Kinnunen, Päivi

N1 - Project code: 81105

PY - 2014

Y1 - 2014

N2 - Currently known high grade easily-acquirable phosphate rock reserves are depleting. The objective of this study was to evaluate the potential application of bioleaching technology to the recovery of phosphorus from different phosphate ore materials, and isolate indigenous phosphorus-solubilising bacterial strains from them. In the bacterial enrichment and isolation experiments no growth was detected in the medium designed for acidophiles. Heterotrophic phosphorus solubilising isolates were enriched from the fluorapatite concentrate, and all four isolates were most closely related to Burkholderia fungorum. Bioleaching of phosphorus from low grade fluorapatite ore containing 8.2% P2O5 and from fluorapatite concentrate containing 29.8% P2O5 was carried out in shake flasks. Supplemental elemental sulphur was added as an energy source for acid generation. Mixed and pure acidophilic bacterial cultures consisting of iron- and/or sulphur-oxidizing bacteria Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans were used in the experiments. These acidophiles are commonly used in bioleaching of sulphide minerals, but their application to phosphorus solubilisation has until now been limited. Phosphorus leaching was shown to be a pH-dependent phenomenon. Phosphorus bioleaching yields of up to 97% and 28% were obtained in 21 d for low grade fluorapatite ore and concentrate, respectively, in solutions with a 1% solid content. With adaptation, the bacterial mixture was acclimated to suspensions with a 10-30% solid content. These results indicate a potential for the application of bioleaching to phosphorus extraction of low grade materials.

AB - Currently known high grade easily-acquirable phosphate rock reserves are depleting. The objective of this study was to evaluate the potential application of bioleaching technology to the recovery of phosphorus from different phosphate ore materials, and isolate indigenous phosphorus-solubilising bacterial strains from them. In the bacterial enrichment and isolation experiments no growth was detected in the medium designed for acidophiles. Heterotrophic phosphorus solubilising isolates were enriched from the fluorapatite concentrate, and all four isolates were most closely related to Burkholderia fungorum. Bioleaching of phosphorus from low grade fluorapatite ore containing 8.2% P2O5 and from fluorapatite concentrate containing 29.8% P2O5 was carried out in shake flasks. Supplemental elemental sulphur was added as an energy source for acid generation. Mixed and pure acidophilic bacterial cultures consisting of iron- and/or sulphur-oxidizing bacteria Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans and Leptospirillum ferrooxidans were used in the experiments. These acidophiles are commonly used in bioleaching of sulphide minerals, but their application to phosphorus solubilisation has until now been limited. Phosphorus leaching was shown to be a pH-dependent phenomenon. Phosphorus bioleaching yields of up to 97% and 28% were obtained in 21 d for low grade fluorapatite ore and concentrate, respectively, in solutions with a 1% solid content. With adaptation, the bacterial mixture was acclimated to suspensions with a 10-30% solid content. These results indicate a potential for the application of bioleaching to phosphorus extraction of low grade materials.

KW - phosphorus

KW - apatite

KW - bioleaching

KW - low-grade ores

KW - acidithiobacillus

U2 - 10.1016/j.hydromet.2014.08.002

DO - 10.1016/j.hydromet.2014.08.002

M3 - Article

VL - 150

SP - 269

EP - 275

JO - Hydrometallurgy

JF - Hydrometallurgy

SN - 0304-386X

ER -