Bioreducible hydrophobin-stabilized supraparticles for selective intracellular release

Daniele Maiolo, Claudia Pigliacelli, Paola Sánchez-Moreno, Martina Violatto, Laura Talamini, Ilaria Tirotta, Rosanna Piccirillo, Massimo Zucchetti, Lavinia Morosi, Roberta Frapolli, Gabriele Candiani, Paolo Bigini, Pierangelo Metrangolo, Francesca Baldelli Bombelli

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

One of the main hurdles in nanomedicine is the low stability of drug-nanocarrier complexes as well as the drug delivery efficiency in the region-of-interest. Here, we describe the use of the film-forming protein hydrophobin HFBII to organize dodecanethiol-protected gold nanoparticles (NPs) into well-defined supraparticles (SPs). The obtained SPs are exceptionally stable in vivo and efficiently encapsulate hydrophobic drug molecules. The HFBII film prevents massive release of the encapsulated drug, which, instead, is activated by selective SP disassembly triggered intracellularly by glutathione reduction of the protein film. As a consequence, the therapeutic efficiency of an encapsulated anticancer drug is highly enhanced (2 orders of magnitude decrease in IC50). Biodistribution and pharmacokinetics studies demonstrate the high stability of the loaded SPs in the bloodstream and the selective release of the payloads once taken up in the tissues. Overall, our results provide a rationale for the development of bioreducible and multifunctional nanomedicines.
Original languageEnglish
Pages (from-to)9413-9423
Number of pages11
JournalACS Nano
Volume11
Issue number9
DOIs
Publication statusPublished - 2017
MoE publication typeA1 Journal article-refereed

Fingerprint

Medical nanotechnology
drugs
Pharmaceutical Preparations
Proteins
Pharmacokinetics
Drug delivery
proteins
Gold
glutathione
Tissue
Nanoparticles
Glutathione
Molecules
payloads
delivery
gold
nanoparticles
molecules

Keywords

  • drug release
  • gold nanoparticle
  • hydrophobin
  • nanobio interface
  • self-assembly
  • supraparticle

Cite this

Maiolo, D., Pigliacelli, C., Sánchez-Moreno, P., Violatto, M., Talamini, L., Tirotta, I., ... Baldelli Bombelli, F. (2017). Bioreducible hydrophobin-stabilized supraparticles for selective intracellular release. ACS Nano, 11(9), 9413-9423. https://doi.org/10.1021/acsnano.7b04979
Maiolo, Daniele ; Pigliacelli, Claudia ; Sánchez-Moreno, Paola ; Violatto, Martina ; Talamini, Laura ; Tirotta, Ilaria ; Piccirillo, Rosanna ; Zucchetti, Massimo ; Morosi, Lavinia ; Frapolli, Roberta ; Candiani, Gabriele ; Bigini, Paolo ; Metrangolo, Pierangelo ; Baldelli Bombelli, Francesca. / Bioreducible hydrophobin-stabilized supraparticles for selective intracellular release. In: ACS Nano. 2017 ; Vol. 11, No. 9. pp. 9413-9423.
@article{7b8c35787c69476fa2a1eb75dab65065,
title = "Bioreducible hydrophobin-stabilized supraparticles for selective intracellular release",
abstract = "One of the main hurdles in nanomedicine is the low stability of drug-nanocarrier complexes as well as the drug delivery efficiency in the region-of-interest. Here, we describe the use of the film-forming protein hydrophobin HFBII to organize dodecanethiol-protected gold nanoparticles (NPs) into well-defined supraparticles (SPs). The obtained SPs are exceptionally stable in vivo and efficiently encapsulate hydrophobic drug molecules. The HFBII film prevents massive release of the encapsulated drug, which, instead, is activated by selective SP disassembly triggered intracellularly by glutathione reduction of the protein film. As a consequence, the therapeutic efficiency of an encapsulated anticancer drug is highly enhanced (2 orders of magnitude decrease in IC50). Biodistribution and pharmacokinetics studies demonstrate the high stability of the loaded SPs in the bloodstream and the selective release of the payloads once taken up in the tissues. Overall, our results provide a rationale for the development of bioreducible and multifunctional nanomedicines.",
keywords = "drug release, gold nanoparticle, hydrophobin, nanobio interface, self-assembly, supraparticle",
author = "Daniele Maiolo and Claudia Pigliacelli and Paola S{\'a}nchez-Moreno and Martina Violatto and Laura Talamini and Ilaria Tirotta and Rosanna Piccirillo and Massimo Zucchetti and Lavinia Morosi and Roberta Frapolli and Gabriele Candiani and Paolo Bigini and Pierangelo Metrangolo and {Baldelli Bombelli}, Francesca",
year = "2017",
doi = "10.1021/acsnano.7b04979",
language = "English",
volume = "11",
pages = "9413--9423",
journal = "ACS Nano",
issn = "1936-0851",
publisher = "American Chemical Society",
number = "9",

}

Maiolo, D, Pigliacelli, C, Sánchez-Moreno, P, Violatto, M, Talamini, L, Tirotta, I, Piccirillo, R, Zucchetti, M, Morosi, L, Frapolli, R, Candiani, G, Bigini, P, Metrangolo, P & Baldelli Bombelli, F 2017, 'Bioreducible hydrophobin-stabilized supraparticles for selective intracellular release', ACS Nano, vol. 11, no. 9, pp. 9413-9423. https://doi.org/10.1021/acsnano.7b04979

Bioreducible hydrophobin-stabilized supraparticles for selective intracellular release. / Maiolo, Daniele; Pigliacelli, Claudia; Sánchez-Moreno, Paola; Violatto, Martina; Talamini, Laura; Tirotta, Ilaria; Piccirillo, Rosanna; Zucchetti, Massimo; Morosi, Lavinia; Frapolli, Roberta; Candiani, Gabriele; Bigini, Paolo; Metrangolo, Pierangelo; Baldelli Bombelli, Francesca.

In: ACS Nano, Vol. 11, No. 9, 2017, p. 9413-9423.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Bioreducible hydrophobin-stabilized supraparticles for selective intracellular release

AU - Maiolo, Daniele

AU - Pigliacelli, Claudia

AU - Sánchez-Moreno, Paola

AU - Violatto, Martina

AU - Talamini, Laura

AU - Tirotta, Ilaria

AU - Piccirillo, Rosanna

AU - Zucchetti, Massimo

AU - Morosi, Lavinia

AU - Frapolli, Roberta

AU - Candiani, Gabriele

AU - Bigini, Paolo

AU - Metrangolo, Pierangelo

AU - Baldelli Bombelli, Francesca

PY - 2017

Y1 - 2017

N2 - One of the main hurdles in nanomedicine is the low stability of drug-nanocarrier complexes as well as the drug delivery efficiency in the region-of-interest. Here, we describe the use of the film-forming protein hydrophobin HFBII to organize dodecanethiol-protected gold nanoparticles (NPs) into well-defined supraparticles (SPs). The obtained SPs are exceptionally stable in vivo and efficiently encapsulate hydrophobic drug molecules. The HFBII film prevents massive release of the encapsulated drug, which, instead, is activated by selective SP disassembly triggered intracellularly by glutathione reduction of the protein film. As a consequence, the therapeutic efficiency of an encapsulated anticancer drug is highly enhanced (2 orders of magnitude decrease in IC50). Biodistribution and pharmacokinetics studies demonstrate the high stability of the loaded SPs in the bloodstream and the selective release of the payloads once taken up in the tissues. Overall, our results provide a rationale for the development of bioreducible and multifunctional nanomedicines.

AB - One of the main hurdles in nanomedicine is the low stability of drug-nanocarrier complexes as well as the drug delivery efficiency in the region-of-interest. Here, we describe the use of the film-forming protein hydrophobin HFBII to organize dodecanethiol-protected gold nanoparticles (NPs) into well-defined supraparticles (SPs). The obtained SPs are exceptionally stable in vivo and efficiently encapsulate hydrophobic drug molecules. The HFBII film prevents massive release of the encapsulated drug, which, instead, is activated by selective SP disassembly triggered intracellularly by glutathione reduction of the protein film. As a consequence, the therapeutic efficiency of an encapsulated anticancer drug is highly enhanced (2 orders of magnitude decrease in IC50). Biodistribution and pharmacokinetics studies demonstrate the high stability of the loaded SPs in the bloodstream and the selective release of the payloads once taken up in the tissues. Overall, our results provide a rationale for the development of bioreducible and multifunctional nanomedicines.

KW - drug release

KW - gold nanoparticle

KW - hydrophobin

KW - nanobio interface

KW - self-assembly

KW - supraparticle

UR - http://www.scopus.com/inward/record.url?scp=85029915850&partnerID=8YFLogxK

U2 - 10.1021/acsnano.7b04979

DO - 10.1021/acsnano.7b04979

M3 - Article

VL - 11

SP - 9413

EP - 9423

JO - ACS Nano

JF - ACS Nano

SN - 1936-0851

IS - 9

ER -

Maiolo D, Pigliacelli C, Sánchez-Moreno P, Violatto M, Talamini L, Tirotta I et al. Bioreducible hydrophobin-stabilized supraparticles for selective intracellular release. ACS Nano. 2017;11(9):9413-9423. https://doi.org/10.1021/acsnano.7b04979