Biosensing devices based on nanostructures and graphene facilitate label-free detection with sensitivities beyond traditional methods. Specific biorecognition relies on selective immobilization of analytes on the close vicinity of the sensor surface. We demonstrate single-step functionalization of graphene biosensors by using surface-active hydrophobin proteins that have been biologically fused with a receptor moiety. The fusion proteins self-align to form a dense and specifically oriented receptor monolayer and can be easily detached from the surface for sensor re-programming. Detection sensitivities in high ionic strength buffer are in femtomolar range both for small charged peptide analytes and for large immunoglobulin antibodies.
|Publication status||Published - 2014|
|Event||2014 MRS Spring Meeting and Exhibit - San Francisco, United States|
Duration: 21 Apr 2014 → 25 Apr 2014
|Conference||2014 MRS Spring Meeting and Exhibit|
|Period||21/04/14 → 25/04/14|
- fusion protein
Kainlauri, M., Kurppa, K., Soikkeli, M., Arpiainen, S., Gunnarsson, D., Joensuu, J., Laaksonen, P., Prunnila, M., Linder, M., & Ahopelto, J. (2014). Biosensing by programmable proteins on graphene field effect transistor. Poster session presented at 2014 MRS Spring Meeting and Exhibit, San Francisco, United States.