Biosensing by programmable proteins on graphene field effect transistor

Markku Kainlauri, Katri Kurppa, Miika Soikkeli, Sanna Arpiainen, David Gunnarsson, Jussi Joensuu, Päivi Laaksonen, Mika Prunnila, M. Linder, Jouni Ahopelto

Research output: Contribution to conferenceConference PosterScientific

Abstract

Biosensing devices based on nanostructures and graphene facilitate label-free detection with sensitivities beyond traditional methods. Specific biorecognition relies on selective immobilization of analytes on the close vicinity of the sensor surface. We demonstrate single-step functionalization of graphene biosensors by using surface-active hydrophobin proteins that have been biologically fused with a receptor moiety. The fusion proteins self-align to form a dense and specifically oriented receptor monolayer and can be easily detached from the surface for sensor re-programming. Detection sensitivities in high ionic strength buffer are in femtomolar range both for small charged peptide analytes and for large immunoglobulin antibodies.
Original languageEnglish
Publication statusPublished - 2014
Event2014 MRS Spring Meeting and Exhibit - San Francisco, United States
Duration: 21 Apr 201425 Apr 2014

Conference

Conference2014 MRS Spring Meeting and Exhibit
CountryUnited States
CitySan Francisco
Period21/04/1425/04/14

Fingerprint

Graphite
Field effect transistors
Proteins
Sensors
Ionic strength
Biosensors
Immunoglobulins
Labels
Monolayers
Nanostructures
Buffers
Fusion reactions
Peptides
Antibodies

Keywords

  • graphene
  • hydrophobin
  • fusion protein
  • biosensor

Cite this

Kainlauri, M., Kurppa, K., Soikkeli, M., Arpiainen, S., Gunnarsson, D., Joensuu, J., ... Ahopelto, J. (2014). Biosensing by programmable proteins on graphene field effect transistor. Poster session presented at 2014 MRS Spring Meeting and Exhibit, San Francisco, United States.
Kainlauri, Markku ; Kurppa, Katri ; Soikkeli, Miika ; Arpiainen, Sanna ; Gunnarsson, David ; Joensuu, Jussi ; Laaksonen, Päivi ; Prunnila, Mika ; Linder, M. ; Ahopelto, Jouni. / Biosensing by programmable proteins on graphene field effect transistor. Poster session presented at 2014 MRS Spring Meeting and Exhibit, San Francisco, United States.
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abstract = "Biosensing devices based on nanostructures and graphene facilitate label-free detection with sensitivities beyond traditional methods. Specific biorecognition relies on selective immobilization of analytes on the close vicinity of the sensor surface. We demonstrate single-step functionalization of graphene biosensors by using surface-active hydrophobin proteins that have been biologically fused with a receptor moiety. The fusion proteins self-align to form a dense and specifically oriented receptor monolayer and can be easily detached from the surface for sensor re-programming. Detection sensitivities in high ionic strength buffer are in femtomolar range both for small charged peptide analytes and for large immunoglobulin antibodies.",
keywords = "graphene, hydrophobin, fusion protein, biosensor",
author = "Markku Kainlauri and Katri Kurppa and Miika Soikkeli and Sanna Arpiainen and David Gunnarsson and Jussi Joensuu and P{\"a}ivi Laaksonen and Mika Prunnila and M. Linder and Jouni Ahopelto",
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Kainlauri, M, Kurppa, K, Soikkeli, M, Arpiainen, S, Gunnarsson, D, Joensuu, J, Laaksonen, P, Prunnila, M, Linder, M & Ahopelto, J 2014, 'Biosensing by programmable proteins on graphene field effect transistor' 2014 MRS Spring Meeting and Exhibit, San Francisco, United States, 21/04/14 - 25/04/14, .

Biosensing by programmable proteins on graphene field effect transistor. / Kainlauri, Markku; Kurppa, Katri; Soikkeli, Miika; Arpiainen, Sanna; Gunnarsson, David; Joensuu, Jussi; Laaksonen, Päivi; Prunnila, Mika; Linder, M.; Ahopelto, Jouni.

2014. Poster session presented at 2014 MRS Spring Meeting and Exhibit, San Francisco, United States.

Research output: Contribution to conferenceConference PosterScientific

TY - CONF

T1 - Biosensing by programmable proteins on graphene field effect transistor

AU - Kainlauri, Markku

AU - Kurppa, Katri

AU - Soikkeli, Miika

AU - Arpiainen, Sanna

AU - Gunnarsson, David

AU - Joensuu, Jussi

AU - Laaksonen, Päivi

AU - Prunnila, Mika

AU - Linder, M.

AU - Ahopelto, Jouni

N1 - Project code: 73337

PY - 2014

Y1 - 2014

N2 - Biosensing devices based on nanostructures and graphene facilitate label-free detection with sensitivities beyond traditional methods. Specific biorecognition relies on selective immobilization of analytes on the close vicinity of the sensor surface. We demonstrate single-step functionalization of graphene biosensors by using surface-active hydrophobin proteins that have been biologically fused with a receptor moiety. The fusion proteins self-align to form a dense and specifically oriented receptor monolayer and can be easily detached from the surface for sensor re-programming. Detection sensitivities in high ionic strength buffer are in femtomolar range both for small charged peptide analytes and for large immunoglobulin antibodies.

AB - Biosensing devices based on nanostructures and graphene facilitate label-free detection with sensitivities beyond traditional methods. Specific biorecognition relies on selective immobilization of analytes on the close vicinity of the sensor surface. We demonstrate single-step functionalization of graphene biosensors by using surface-active hydrophobin proteins that have been biologically fused with a receptor moiety. The fusion proteins self-align to form a dense and specifically oriented receptor monolayer and can be easily detached from the surface for sensor re-programming. Detection sensitivities in high ionic strength buffer are in femtomolar range both for small charged peptide analytes and for large immunoglobulin antibodies.

KW - graphene

KW - hydrophobin

KW - fusion protein

KW - biosensor

UR - https://www.mrs.org/spring-2014

M3 - Conference Poster

ER -

Kainlauri M, Kurppa K, Soikkeli M, Arpiainen S, Gunnarsson D, Joensuu J et al. Biosensing by programmable proteins on graphene field effect transistor. 2014. Poster session presented at 2014 MRS Spring Meeting and Exhibit, San Francisco, United States.