Biosensing by programmable proteins on graphene field effect transistor

Markku Kainlauri, Katri Kurppa, Miika Soikkeli, Sanna Arpiainen, David Gunnarsson, Jussi Joensuu, Päivi Laaksonen, Mika Prunnila, M. Linder, Jouni Ahopelto

Research output: Contribution to conferenceConference PosterScientific

Abstract

Biosensing devices based on nanostructures and graphene facilitate label-free detection with sensitivities beyond traditional methods. Specific biorecognition relies on selective immobilization of analytes on the close vicinity of the sensor surface. We demonstrate single-step functionalization of graphene biosensors by using surface-active hydrophobin proteins that have been biologically fused with a receptor moiety. The fusion proteins self-align to form a dense and specifically oriented receptor monolayer and can be easily detached from the surface for sensor re-programming. Detection sensitivities in high ionic strength buffer are in femtomolar range both for small charged peptide analytes and for large immunoglobulin antibodies.
Original languageEnglish
Publication statusPublished - 2014
Event2014 MRS Spring Meeting and Exhibit - San Francisco, United States
Duration: 21 Apr 201425 Apr 2014

Conference

Conference2014 MRS Spring Meeting and Exhibit
CountryUnited States
CitySan Francisco
Period21/04/1425/04/14

Keywords

  • graphene
  • hydrophobin
  • fusion protein
  • biosensor

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