Biosensing by programmable proteins on graphene field effect transistor

Markku Kainlauri, Katri Kurppa, Miika Soikkeli, Sanna Arpiainen, David Gunnarsson, Jussi Joensuu, Päivi Laaksonen, Mika Prunnila, M. Linder, Jouni Ahopelto

    Research output: Contribution to conferenceConference PosterScientific


    Biosensing devices based on nanostructures and graphene facilitate label-free detection with sensitivities beyond traditional methods. Specific biorecognition relies on selective immobilization of analytes on the close vicinity of the sensor surface. We demonstrate single-step functionalization of graphene biosensors by using surface-active hydrophobin proteins that have been biologically fused with a receptor moiety. The fusion proteins self-align to form a dense and specifically oriented receptor monolayer and can be easily detached from the surface for sensor re-programming. Detection sensitivities in high ionic strength buffer are in femtomolar range both for small charged peptide analytes and for large immunoglobulin antibodies.
    Original languageEnglish
    Publication statusPublished - 2014
    Event2014 MRS Spring Meeting and Exhibit - San Francisco, United States
    Duration: 21 Apr 201425 Apr 2014


    Conference2014 MRS Spring Meeting and Exhibit
    Country/TerritoryUnited States
    CitySan Francisco


    • graphene
    • hydrophobin
    • fusion protein
    • biosensor


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