@inproceedings{0ae8e246d56644c5a539294e1f7e86b7,
title = "Biosynthesis of the antifungal polyketide antibiotic soraphen A in Sorangium cellulosum and Streptomyces lividans",
abstract = "The derivatization of soraphen by combinatorial biosynthesis has been a longstanding goal of the Natural Product Genetics group at Ciba Geigy - Novartis - Syngenta. We have cloned and characterized the soraphen A biosynthetic gene cluster of Sorangium cellulosum So ce26. To facilitate the genetic manipulation of So ce26, a central regulator of gliding motility was identified and its encoding gene disrupted to create a nonswarming strain. Despite this improvement, engineering of soraphen biosynthesis remained elusive in So ce26. Thus, the genes for the soraphen polyketide synthase, the post-polyketide tailoring steps, and methoxymalonate biosynthesis were introduced into Streptomyces lividans. Production of soraphen A in this genetically tractable strain also required provision of benzoyl-CoA by feeding benzoate or cinnamate.",
author = "Istv{\'a}n Moln{\'a}r and Ross Zirkle and Lig{\'o}n, {James M.}",
year = "2007",
doi = "10.1021/bk-2007-0955.ch015",
language = "English",
isbn = "978-0-8412-3978-4",
series = "ACS Symposium Series",
publisher = "American Chemical Society ACS",
pages = "217--230",
booktitle = "Polyketides Biosynthesis, Biological Activity, and Genetic Engineering",
address = "United States",
}