Biotechnical utilization of wood carbohydrates after steaming pretreatment

Jurgen Puls, Kaisa Poutanen, Hans-Ulrich Körner, Liisa Viikari

Research output: Contribution to journalArticleScientificpeer-review

170 Citations (Scopus)

Abstract

Birch wood was used as raw material to study the effect of steaming pretreatment on the characteristics, enzymatic hydrolysis and fermentation of cellulose and hemicellulose. The cellulose remained undissolved in the fibres after steaming, but the degree of polymerization decreased and the surface area increased with increasing steaming temperature. The yield in enzymatic hydrolysis with T. reesei and A. niger cellulases increased from 40 to 75% of theoretical when the pretreatment temperature was increased from 170 to 210°C at a residence time of 10 minutes. The glucose released was fermented to ethanol by yeast without interference of toxic compounds. After steaming, the hemicellulose was mainly in the form of xylo-oligomers. The average chain length decreased with increasing temperature. Only the monomeric sugars were fermented to ethanol by Fusarium oxysporum. After steaming at 210°C toxic decomposition products inhibited the fermentation completely. In aerobic conditions also the xylo-oligomers were metabolized.

Original languageEnglish
Pages (from-to)416 - 423
Number of pages8
JournalApplied Microbiology and Biotechnology
Volume22
Issue number6
DOIs
Publication statusPublished - 1985
MoE publication typeNot Eligible

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Poisons
Carbohydrates
Cellulose
Fermentation
Temperature
Hydrolysis
Ethanol
Cellulases
Betula
Fusarium
Polymerization
Yeasts
Glucose
hemicellulose

Cite this

Puls, Jurgen ; Poutanen, Kaisa ; Körner, Hans-Ulrich ; Viikari, Liisa. / Biotechnical utilization of wood carbohydrates after steaming pretreatment. In: Applied Microbiology and Biotechnology. 1985 ; Vol. 22, No. 6. pp. 416 - 423.
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Biotechnical utilization of wood carbohydrates after steaming pretreatment. / Puls, Jurgen; Poutanen, Kaisa; Körner, Hans-Ulrich; Viikari, Liisa.

In: Applied Microbiology and Biotechnology, Vol. 22, No. 6, 1985, p. 416 - 423.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Biotechnical utilization of wood carbohydrates after steaming pretreatment

AU - Puls, Jurgen

AU - Poutanen, Kaisa

AU - Körner, Hans-Ulrich

AU - Viikari, Liisa

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AB - Birch wood was used as raw material to study the effect of steaming pretreatment on the characteristics, enzymatic hydrolysis and fermentation of cellulose and hemicellulose. The cellulose remained undissolved in the fibres after steaming, but the degree of polymerization decreased and the surface area increased with increasing steaming temperature. The yield in enzymatic hydrolysis with T. reesei and A. niger cellulases increased from 40 to 75% of theoretical when the pretreatment temperature was increased from 170 to 210°C at a residence time of 10 minutes. The glucose released was fermented to ethanol by yeast without interference of toxic compounds. After steaming, the hemicellulose was mainly in the form of xylo-oligomers. The average chain length decreased with increasing temperature. Only the monomeric sugars were fermented to ethanol by Fusarium oxysporum. After steaming at 210°C toxic decomposition products inhibited the fermentation completely. In aerobic conditions also the xylo-oligomers were metabolized.

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