Biotransformation of hyoscyamine into scopolamine in transgenic tobacco cell cultures

Elisabeth Moyano, Javier Palazon, Mercedes Bonfill, Lidia Osuna, Rosa M. Cusido, Kirsi-Marja Oksman-Caldentey, M. Teresa Pinol

    Research output: Contribution to journalArticleScientificpeer-review

    31 Citations (Scopus)

    Abstract

    Hyoscyamine-6beta-hydroxylase (H6H) catalyses the conversion of hyoscyamine into its epoxide scopolamine, a compound with a higher added value in the pharmaceutical market than hyoscyamine. We report the establishment of tobacco cell cultures carrying the Hyoscyamus muticus h6h gene under the control of the promoter CAMV 35S. The cell cultures were derived from hairy roots obtained via genetically modified Agrobacterium rhizogenes carrying the pRi and pLAL21 plasmids. The cultures were fed with hyoscyamine, and 4 weeks later the amount of scopolamine produced was quantified by HPLC. The transgenic cell suspension cultures showed a considerable capacity for the bioconversion of hyoscyamine into scopolamine, and released it to the culture medium. Although the scale-up from shake-flask to bioreactor culture usually results in reduced productivities, our transgenic cells grown in a 5-L turbine stirred tank reactor in a batch mode significantly increased the scopolamine accumulation.
    Original languageEnglish
    Pages (from-to)521-524
    JournalJournal of Plant Physiology
    Volume164
    Issue number4
    DOIs
    Publication statusPublished - 2007
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Biotransformation
    • Hyoscyamine-6beta-hydroxylase
    • Hyoscyamine
    • Scopolamine
    • Tobacco cell cultures

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