Biotransformation of hyoscyamine into scopolamine in transgenic tobacco cell cultures

Elisabeth Moyano, Javier Palazon, Mercedes Bonfill, Lidia Osuna, Rosa M. Cusido, Kirsi-Marja Oksman-Caldentey, M. Teresa Pinol

Research output: Contribution to journalArticleScientificpeer-review

27 Citations (Scopus)

Abstract

Hyoscyamine-6beta-hydroxylase (H6H) catalyses the conversion of hyoscyamine into its epoxide scopolamine, a compound with a higher added value in the pharmaceutical market than hyoscyamine. We report the establishment of tobacco cell cultures carrying the Hyoscyamus muticus h6h gene under the control of the promoter CAMV 35S. The cell cultures were derived from hairy roots obtained via genetically modified Agrobacterium rhizogenes carrying the pRi and pLAL21 plasmids. The cultures were fed with hyoscyamine, and 4 weeks later the amount of scopolamine produced was quantified by HPLC. The transgenic cell suspension cultures showed a considerable capacity for the bioconversion of hyoscyamine into scopolamine, and released it to the culture medium. Although the scale-up from shake-flask to bioreactor culture usually results in reduced productivities, our transgenic cells grown in a 5-L turbine stirred tank reactor in a batch mode significantly increased the scopolamine accumulation.
Original languageEnglish
Pages (from-to)521-524
JournalJournal of Plant Physiology
Volume164
Issue number4
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Keywords

  • Biotransformation
  • Hyoscyamine-6beta-hydroxylase
  • Hyoscyamine
  • Scopolamine
  • Tobacco cell cultures

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