C- and N-catabolic utilization of tricarboxylic acid cycle-related amino acids by Scheffersomyces stipitis and other yeasts

Stefan Freese (Corresponding Author), Tanja Vogts, Falk Speer, Bernd Schäfer, Volkmar Passoth, Ulrich Klinner

Research output: Contribution to journalArticleScientificpeer-review

17 Citations (Scopus)

Abstract

Scheffersomyces stipitis and the closely related yeast Candida shehatae assimilated the L‐amino acids glutamate, aspartate and proline as both carbon and nitrogen sole sources. We also found this rarely investigated ability in ascomycetous species such as Candida glabrata, C. reukaufii, C. utilis, Debaryomyces hansenii, Kluyveromyces lactis, K. marxianus, Candida albicans, L. elongisporus, Meyerozyma guilliermondii, C. maltosa, Pichia capsulata and Yarrowia lipolytica and in basidiomycetous species such as Rhodotorula rubra and Trichosporon beigelii. Glutamate was a very efficient carbon source for Sc. stipitis, which enabled a high biomass yield/mole, although the growth rate was lower when compared to growth on glucose medium. The cells secreted waste ammonium during growth on glutamate alone. In Sc. stipitis cultures grown in glucose medium containing glutamate as the nitrogen source the biomass yield was maximal, and ethanol concentration and specific ethanol formation rate were significantly higher than in glucose medium containing ammonium as the nitrogen source. Mainly C‐assimilation of glutamate but also N‐assimilation in glucose‐containing medium correlated with enhanced activity of the NAD‐dependent glutamate dehydrogenase 2 (GDH2). A Δgdh2 disruptant was unable to utilize glutamate as either a carbon or a nitrogen source; moreover, this disruptant was also unable to utilize aspartate as a carbon source. The mutation was complemented by retransformation of the GDH2 ORF into the Δgdh2 strain. The results show that Gdh2p plays a dual role in Sc. stipitis as both C‐ and N‐catabolic enzyme, which indicates its role as an interface between the carbon and nitrogen metabolism of this yeast.
Original languageEnglish
Pages (from-to)375-390
JournalYeast
Volume28
Issue number5
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

Fingerprint

Citric Acid Cycle
Yeast
Amino acids
Glutamic Acid
Yeasts
Candida
Nitrogen
Carbon
Amino Acids
Glucose
Glutamate Dehydrogenase
Ammonium Compounds
Biomass
Aspartic Acid
Ethanol
Growth
Yarrowia
Trichosporon
Rhodotorula
Kluyveromyces

Keywords

  • Scheffersomyces stipitis
  • amino acids
  • carbon sources
  • nitrogen sources
  • GDH1
  • GDH2
  • GDH3

Cite this

Freese, Stefan ; Vogts, Tanja ; Speer, Falk ; Schäfer, Bernd ; Passoth, Volkmar ; Klinner, Ulrich. / C- and N-catabolic utilization of tricarboxylic acid cycle-related amino acids by Scheffersomyces stipitis and other yeasts. In: Yeast. 2011 ; Vol. 28, No. 5. pp. 375-390.
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Freese, S, Vogts, T, Speer, F, Schäfer, B, Passoth, V & Klinner, U 2011, 'C- and N-catabolic utilization of tricarboxylic acid cycle-related amino acids by Scheffersomyces stipitis and other yeasts', Yeast, vol. 28, no. 5, pp. 375-390. https://doi.org/10.1002/yea.1845

C- and N-catabolic utilization of tricarboxylic acid cycle-related amino acids by Scheffersomyces stipitis and other yeasts. / Freese, Stefan (Corresponding Author); Vogts, Tanja; Speer, Falk; Schäfer, Bernd; Passoth, Volkmar; Klinner, Ulrich.

In: Yeast, Vol. 28, No. 5, 2011, p. 375-390.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - C- and N-catabolic utilization of tricarboxylic acid cycle-related amino acids by Scheffersomyces stipitis and other yeasts

AU - Freese, Stefan

AU - Vogts, Tanja

AU - Speer, Falk

AU - Schäfer, Bernd

AU - Passoth, Volkmar

AU - Klinner, Ulrich

PY - 2011

Y1 - 2011

N2 - Scheffersomyces stipitis and the closely related yeast Candida shehatae assimilated the L‐amino acids glutamate, aspartate and proline as both carbon and nitrogen sole sources. We also found this rarely investigated ability in ascomycetous species such as Candida glabrata, C. reukaufii, C. utilis, Debaryomyces hansenii, Kluyveromyces lactis, K. marxianus, Candida albicans, L. elongisporus, Meyerozyma guilliermondii, C. maltosa, Pichia capsulata and Yarrowia lipolytica and in basidiomycetous species such as Rhodotorula rubra and Trichosporon beigelii. Glutamate was a very efficient carbon source for Sc. stipitis, which enabled a high biomass yield/mole, although the growth rate was lower when compared to growth on glucose medium. The cells secreted waste ammonium during growth on glutamate alone. In Sc. stipitis cultures grown in glucose medium containing glutamate as the nitrogen source the biomass yield was maximal, and ethanol concentration and specific ethanol formation rate were significantly higher than in glucose medium containing ammonium as the nitrogen source. Mainly C‐assimilation of glutamate but also N‐assimilation in glucose‐containing medium correlated with enhanced activity of the NAD‐dependent glutamate dehydrogenase 2 (GDH2). A Δgdh2 disruptant was unable to utilize glutamate as either a carbon or a nitrogen source; moreover, this disruptant was also unable to utilize aspartate as a carbon source. The mutation was complemented by retransformation of the GDH2 ORF into the Δgdh2 strain. The results show that Gdh2p plays a dual role in Sc. stipitis as both C‐ and N‐catabolic enzyme, which indicates its role as an interface between the carbon and nitrogen metabolism of this yeast.

AB - Scheffersomyces stipitis and the closely related yeast Candida shehatae assimilated the L‐amino acids glutamate, aspartate and proline as both carbon and nitrogen sole sources. We also found this rarely investigated ability in ascomycetous species such as Candida glabrata, C. reukaufii, C. utilis, Debaryomyces hansenii, Kluyveromyces lactis, K. marxianus, Candida albicans, L. elongisporus, Meyerozyma guilliermondii, C. maltosa, Pichia capsulata and Yarrowia lipolytica and in basidiomycetous species such as Rhodotorula rubra and Trichosporon beigelii. Glutamate was a very efficient carbon source for Sc. stipitis, which enabled a high biomass yield/mole, although the growth rate was lower when compared to growth on glucose medium. The cells secreted waste ammonium during growth on glutamate alone. In Sc. stipitis cultures grown in glucose medium containing glutamate as the nitrogen source the biomass yield was maximal, and ethanol concentration and specific ethanol formation rate were significantly higher than in glucose medium containing ammonium as the nitrogen source. Mainly C‐assimilation of glutamate but also N‐assimilation in glucose‐containing medium correlated with enhanced activity of the NAD‐dependent glutamate dehydrogenase 2 (GDH2). A Δgdh2 disruptant was unable to utilize glutamate as either a carbon or a nitrogen source; moreover, this disruptant was also unable to utilize aspartate as a carbon source. The mutation was complemented by retransformation of the GDH2 ORF into the Δgdh2 strain. The results show that Gdh2p plays a dual role in Sc. stipitis as both C‐ and N‐catabolic enzyme, which indicates its role as an interface between the carbon and nitrogen metabolism of this yeast.

KW - Scheffersomyces stipitis

KW - amino acids

KW - carbon sources

KW - nitrogen sources

KW - GDH1

KW - GDH2

KW - GDH3

U2 - 10.1002/yea.1845

DO - 10.1002/yea.1845

M3 - Article

VL - 28

SP - 375

EP - 390

JO - Yeast

JF - Yeast

SN - 0749-503X

IS - 5

ER -