C-Jun N-terminal kinase phosphorylation of MARCKSL1 determines actin stability and migration in neurons and in cancer cells

B. Björkblom, A. Padzik, H. Mohammad, N. Westerlund, E. Komulainen, P. Hollos, L. Parviainen, A.C. Papageorgiou, Kristiina Iljin, O. Kallioniemi, M. Kallajoki, M.J. Courtney, M. Magar, P. James, E.T. Coffey

Research output: Contribution to journalArticleScientificpeer-review

35 Citations (Scopus)

Abstract

Cell migration is a fundamental biological function, critical during development and regeneration, whereas deregulated migration underlies neurological birth defects and cancer metastasis. MARCKS-like protein 1 (MARCKSL1) is widely expressed in nervous tissue, where, like Jun N-terminal protein kinase (JNK), it is required for neural tube formation, though the mechanism is unknown. Here we show that MARCKSL1 is directly phosphorylated by JNK on C-terminal residues (S120, T148, and T183). This phosphorylation enables MARCKSL1 to bundle and stabilize F-actin, increase filopodium numbers and dynamics, and retard migration in neurons. Conversely, when MARCKSL1 phosphorylation is inhibited, actin mobility increases and filopodium formation is compromised whereas lamellipodium formation is enhanced, as is cell migration. We find that MARCKSL1 mRNA is upregulated in a broad range of cancer types and that MARCKSL1 protein is strongly induced in primary prostate carcinomas. Gene knockdown in prostate cancer cells or in neurons reveals a critical role for MARCKSL1 in migration that is dependent on the phosphorylation state; phosphomimetic MARCKSL1 (MARCKSL1S120D,T148D,T183D) inhibits whereas dephospho-MARCKSL1S120A,T148A,T183A induces migration. In summary, these data show that JNK phosphorylation of MARCKSL1 regulates actin homeostasis, filopodium and lamellipodium formation, and neuronal migration under physiological conditions and that, when ectopically expressed in prostate cancer cells, MARCKSL1 again determines cell movement.
Original languageEnglish
Pages (from-to)3513-3526
Number of pages13
JournalMolecular and Cellular Biology
Volume32
Issue number17
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

Actins
Phosphotransferases
Phosphorylation
Neurons
Pseudopodia
Neoplasms
Cell Movement
Prostatic Neoplasms
myristoylated alanine-rich C kinase substrate
Gene Knockdown Techniques
Nerve Tissue
Neural Tube
Protein Kinase C
Prostate
Regeneration
Homeostasis
Neoplasm Metastasis
Carcinoma
Messenger RNA

Cite this

Björkblom, B., Padzik, A., Mohammad, H., Westerlund, N., Komulainen, E., Hollos, P., ... Coffey, E. T. (2012). C-Jun N-terminal kinase phosphorylation of MARCKSL1 determines actin stability and migration in neurons and in cancer cells. Molecular and Cellular Biology, 32(17), 3513-3526. https://doi.org/10.1128/MCB.00713-12
Björkblom, B. ; Padzik, A. ; Mohammad, H. ; Westerlund, N. ; Komulainen, E. ; Hollos, P. ; Parviainen, L. ; Papageorgiou, A.C. ; Iljin, Kristiina ; Kallioniemi, O. ; Kallajoki, M. ; Courtney, M.J. ; Magar, M. ; James, P. ; Coffey, E.T. / C-Jun N-terminal kinase phosphorylation of MARCKSL1 determines actin stability and migration in neurons and in cancer cells. In: Molecular and Cellular Biology. 2012 ; Vol. 32, No. 17. pp. 3513-3526.
@article{f9624a8260724b9093d6d800299ba649,
title = "C-Jun N-terminal kinase phosphorylation of MARCKSL1 determines actin stability and migration in neurons and in cancer cells",
abstract = "Cell migration is a fundamental biological function, critical during development and regeneration, whereas deregulated migration underlies neurological birth defects and cancer metastasis. MARCKS-like protein 1 (MARCKSL1) is widely expressed in nervous tissue, where, like Jun N-terminal protein kinase (JNK), it is required for neural tube formation, though the mechanism is unknown. Here we show that MARCKSL1 is directly phosphorylated by JNK on C-terminal residues (S120, T148, and T183). This phosphorylation enables MARCKSL1 to bundle and stabilize F-actin, increase filopodium numbers and dynamics, and retard migration in neurons. Conversely, when MARCKSL1 phosphorylation is inhibited, actin mobility increases and filopodium formation is compromised whereas lamellipodium formation is enhanced, as is cell migration. We find that MARCKSL1 mRNA is upregulated in a broad range of cancer types and that MARCKSL1 protein is strongly induced in primary prostate carcinomas. Gene knockdown in prostate cancer cells or in neurons reveals a critical role for MARCKSL1 in migration that is dependent on the phosphorylation state; phosphomimetic MARCKSL1 (MARCKSL1S120D,T148D,T183D) inhibits whereas dephospho-MARCKSL1S120A,T148A,T183A induces migration. In summary, these data show that JNK phosphorylation of MARCKSL1 regulates actin homeostasis, filopodium and lamellipodium formation, and neuronal migration under physiological conditions and that, when ectopically expressed in prostate cancer cells, MARCKSL1 again determines cell movement.",
author = "B. Bj{\"o}rkblom and A. Padzik and H. Mohammad and N. Westerlund and E. Komulainen and P. Hollos and L. Parviainen and A.C. Papageorgiou and Kristiina Iljin and O. Kallioniemi and M. Kallajoki and M.J. Courtney and M. Magar and P. James and E.T. Coffey",
year = "2012",
doi = "10.1128/MCB.00713-12",
language = "English",
volume = "32",
pages = "3513--3526",
journal = "Molecular and Cellular Biology",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "17",

}

Björkblom, B, Padzik, A, Mohammad, H, Westerlund, N, Komulainen, E, Hollos, P, Parviainen, L, Papageorgiou, AC, Iljin, K, Kallioniemi, O, Kallajoki, M, Courtney, MJ, Magar, M, James, P & Coffey, ET 2012, 'C-Jun N-terminal kinase phosphorylation of MARCKSL1 determines actin stability and migration in neurons and in cancer cells', Molecular and Cellular Biology, vol. 32, no. 17, pp. 3513-3526. https://doi.org/10.1128/MCB.00713-12

C-Jun N-terminal kinase phosphorylation of MARCKSL1 determines actin stability and migration in neurons and in cancer cells. / Björkblom, B.; Padzik, A.; Mohammad, H.; Westerlund, N.; Komulainen, E.; Hollos, P.; Parviainen, L.; Papageorgiou, A.C.; Iljin, Kristiina; Kallioniemi, O.; Kallajoki, M.; Courtney, M.J.; Magar, M.; James, P.; Coffey, E.T.

In: Molecular and Cellular Biology, Vol. 32, No. 17, 2012, p. 3513-3526.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - C-Jun N-terminal kinase phosphorylation of MARCKSL1 determines actin stability and migration in neurons and in cancer cells

AU - Björkblom, B.

AU - Padzik, A.

AU - Mohammad, H.

AU - Westerlund, N.

AU - Komulainen, E.

AU - Hollos, P.

AU - Parviainen, L.

AU - Papageorgiou, A.C.

AU - Iljin, Kristiina

AU - Kallioniemi, O.

AU - Kallajoki, M.

AU - Courtney, M.J.

AU - Magar, M.

AU - James, P.

AU - Coffey, E.T.

PY - 2012

Y1 - 2012

N2 - Cell migration is a fundamental biological function, critical during development and regeneration, whereas deregulated migration underlies neurological birth defects and cancer metastasis. MARCKS-like protein 1 (MARCKSL1) is widely expressed in nervous tissue, where, like Jun N-terminal protein kinase (JNK), it is required for neural tube formation, though the mechanism is unknown. Here we show that MARCKSL1 is directly phosphorylated by JNK on C-terminal residues (S120, T148, and T183). This phosphorylation enables MARCKSL1 to bundle and stabilize F-actin, increase filopodium numbers and dynamics, and retard migration in neurons. Conversely, when MARCKSL1 phosphorylation is inhibited, actin mobility increases and filopodium formation is compromised whereas lamellipodium formation is enhanced, as is cell migration. We find that MARCKSL1 mRNA is upregulated in a broad range of cancer types and that MARCKSL1 protein is strongly induced in primary prostate carcinomas. Gene knockdown in prostate cancer cells or in neurons reveals a critical role for MARCKSL1 in migration that is dependent on the phosphorylation state; phosphomimetic MARCKSL1 (MARCKSL1S120D,T148D,T183D) inhibits whereas dephospho-MARCKSL1S120A,T148A,T183A induces migration. In summary, these data show that JNK phosphorylation of MARCKSL1 regulates actin homeostasis, filopodium and lamellipodium formation, and neuronal migration under physiological conditions and that, when ectopically expressed in prostate cancer cells, MARCKSL1 again determines cell movement.

AB - Cell migration is a fundamental biological function, critical during development and regeneration, whereas deregulated migration underlies neurological birth defects and cancer metastasis. MARCKS-like protein 1 (MARCKSL1) is widely expressed in nervous tissue, where, like Jun N-terminal protein kinase (JNK), it is required for neural tube formation, though the mechanism is unknown. Here we show that MARCKSL1 is directly phosphorylated by JNK on C-terminal residues (S120, T148, and T183). This phosphorylation enables MARCKSL1 to bundle and stabilize F-actin, increase filopodium numbers and dynamics, and retard migration in neurons. Conversely, when MARCKSL1 phosphorylation is inhibited, actin mobility increases and filopodium formation is compromised whereas lamellipodium formation is enhanced, as is cell migration. We find that MARCKSL1 mRNA is upregulated in a broad range of cancer types and that MARCKSL1 protein is strongly induced in primary prostate carcinomas. Gene knockdown in prostate cancer cells or in neurons reveals a critical role for MARCKSL1 in migration that is dependent on the phosphorylation state; phosphomimetic MARCKSL1 (MARCKSL1S120D,T148D,T183D) inhibits whereas dephospho-MARCKSL1S120A,T148A,T183A induces migration. In summary, these data show that JNK phosphorylation of MARCKSL1 regulates actin homeostasis, filopodium and lamellipodium formation, and neuronal migration under physiological conditions and that, when ectopically expressed in prostate cancer cells, MARCKSL1 again determines cell movement.

U2 - 10.1128/MCB.00713-12

DO - 10.1128/MCB.00713-12

M3 - Article

VL - 32

SP - 3513

EP - 3526

JO - Molecular and Cellular Biology

JF - Molecular and Cellular Biology

SN - 0270-7306

IS - 17

ER -