Calcium-induced structural changes and domain autonomy in calmodulin

B. Finn, J. Evenäs, Torbjörn Drakenberg, J. Waltho, Eva Thulin, Sture Forsen

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Abstract

We have determined the solution structures of the apo and (Ca2+)2 forms of the carboxy-terminal domain of calmodulin using multidimensional heteronuclear nuclear magnetic resonance spectroscopy.
The results show that both forms adopt well-defined structures with essentially equal secondary structure. A comparison of the structures of the two forms shows that Ca2+ binding causes major rearrangements of the secondary structure elements with changes in inter-residue distances of up to 15 A and exposure of the hydrophobic interior of the four-helix bundle.
Comparisons with previously determined high-resolution X-ray structures and models of calmodulin indicate that this domain is structurally autonomous.
Original languageEnglish
Pages (from-to)777-783
JournalNature Structural Biology
Volume2
Issue number9
DOIs
Publication statusPublished - 1995
MoE publication typeA1 Journal article-refereed

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Calmodulin
Biomolecular Nuclear Magnetic Resonance
Calcium
Magnetic Resonance Spectroscopy
X-Rays

Cite this

Finn, B., Evenäs, J., Drakenberg, T., Waltho, J., Thulin, E., & Forsen, S. (1995). Calcium-induced structural changes and domain autonomy in calmodulin. Nature Structural Biology, 2(9), 777-783. https://doi.org/10.1038/nsb0995-777
Finn, B. ; Evenäs, J. ; Drakenberg, Torbjörn ; Waltho, J. ; Thulin, Eva ; Forsen, Sture. / Calcium-induced structural changes and domain autonomy in calmodulin. In: Nature Structural Biology. 1995 ; Vol. 2, No. 9. pp. 777-783.
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Finn, B, Evenäs, J, Drakenberg, T, Waltho, J, Thulin, E & Forsen, S 1995, 'Calcium-induced structural changes and domain autonomy in calmodulin', Nature Structural Biology, vol. 2, no. 9, pp. 777-783. https://doi.org/10.1038/nsb0995-777

Calcium-induced structural changes and domain autonomy in calmodulin. / Finn, B.; Evenäs, J.; Drakenberg, Torbjörn; Waltho, J.; Thulin, Eva; Forsen, Sture.

In: Nature Structural Biology, Vol. 2, No. 9, 1995, p. 777-783.

Research output: Contribution to journalArticleScientificpeer-review

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Finn B, Evenäs J, Drakenberg T, Waltho J, Thulin E, Forsen S. Calcium-induced structural changes and domain autonomy in calmodulin. Nature Structural Biology. 1995;2(9):777-783. https://doi.org/10.1038/nsb0995-777