Candidate genes for testicular cancer evaluated by in situ protein expression analyses on tissue microarrays

Rolf I. Skotheim, Vera M. Abeler, Jahn M. Nesland, Sophie D. Fosså, Ruth Holm, Urs Wagner, Vivi Ann Florenes, Nina Aass, Olli Kallioniemi, Ragnhild A. Lothe (Corresponding Author)

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Abstract

By the use of high-throughput molecular technologies, the number of genes and proteins potentially relevant to testicular germ cell tumor (TGCT) and other diseases will increase rapidly. In a recent transcriptional profiling, we demonstrated the overexpression of GRB7 and JUP in TGCTs, confirmed the reported overexpression of CCND2. We also have recent evidences for frequent genetic alterations of FHIT and epigenetic alterations of MGMT. To evaluate whether the expression of these genes is related to any clinicopathological variables, we constructed a tissue microarray with 510 testicular tissue cores from 279 patients diagnosed with TGCT, covering various histological subgroups and clinical stages. By immunohistochemistry, we found that JUP, GRB7, CCND2 proteins were rarely present in normal testis, but frequently expressed at high levels in TGCT. Additionally, all premalignant intratubular germ cell neoplasias were JUP-immunopositive. MGMT and FHIT were expressed by normal testicular tissues, but at significantly lower frequencies in TGCT. Except for CCND2, the expressions of all markers were significantly associated with various TGCT subtypes. In summary, we have developed a high-throughput tool for the evaluation of TGCT markers, utilized this to validate five candidate genes whose protein expressions were indeed deregulated in TGCT.
Original languageEnglish
Pages (from-to)397-404
JournalNeoplasia
Volume5
Issue number5
DOIs
Publication statusPublished - 2003
MoE publication typeA1 Journal article-refereed

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    Skotheim, R. I., Abeler, V. M., Nesland, J. M., Fosså, S. D., Holm, R., Wagner, U., Florenes, V. A., Aass, N., Kallioniemi, O., & Lothe, R. A. (2003). Candidate genes for testicular cancer evaluated by in situ protein expression analyses on tissue microarrays. Neoplasia, 5(5), 397-404. https://doi.org/10.1016/S1476-5586(03)80042-8