Capacitance DNA bio-chips improved by new probe immobilization strategies

Sandra Carrara (Corresponding Author), Andrea Cavallini, Yusuf Leblebici, Giovanni De Micheli, Vijayender Bhalla, Francesco Valle, Bruno Samorì, Luca Benini, Bruno Riccò, Inger Vikholm-Lundin, Tony Munter

Research output: Contribution to journalArticleScientificpeer-review

8 Citations (Scopus)

Abstract

Label-free DNA detection plays a crucial role in developing point-of-care biochips. Capacitance detection is a promising technology for label-free detection. However, data published in literature often show evident time drift, large standard deviation, scattered data points, and poor reproducibility. To address these problems, mercapto-hexanol or similar alkanethiols are usually considered as blocking agents. The aim of the present paper is to investigate new blocking agents to further improve DNA probe surfaces. Data from AFM, SPR, florescence microscopy, and capacitance measurements are used to investigate new lipoate and ethylene-glycol molecules. The new surfaces offer further improvements in terms of diminished detection errors. Film structures are investigated at the nano-scale to justify the detection improvements in terms of probe surface quality. This study demonstrates the superiority of lipoate and ethylene-glycol molecules as blocking candidates when immobilizing molecular probes onto spot surfaces in label-free DNA biochip.
Original languageEnglish
Pages (from-to)711-717
JournalMicroelectronics Journal
Volume41
Issue number11
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

Fingerprint

immobilization
Biochips
Labels
DNA
Capacitance
Ethylene Glycol
deoxyribonucleic acid
capacitance
chips
Ethylene glycol
probes
Hexanols
Molecular Probes
Molecules
Capacitance measurement
Error detection
DNA Probes
glycols
ethylene
Surface properties

Keywords

  • Alkanethiols
  • Biochip
  • DNA immobilization
  • Ethylene-glycol
  • Lipoate-diethanolamines
  • Mercapto-hexanol

Cite this

Carrara, S., Cavallini, A., Leblebici, Y., De Micheli, G., Bhalla, V., Valle, F., ... Munter, T. (2010). Capacitance DNA bio-chips improved by new probe immobilization strategies. Microelectronics Journal, 41(11), 711-717. https://doi.org/10.1016/j.mejo.2010.01.007
Carrara, Sandra ; Cavallini, Andrea ; Leblebici, Yusuf ; De Micheli, Giovanni ; Bhalla, Vijayender ; Valle, Francesco ; Samorì, Bruno ; Benini, Luca ; Riccò, Bruno ; Vikholm-Lundin, Inger ; Munter, Tony. / Capacitance DNA bio-chips improved by new probe immobilization strategies. In: Microelectronics Journal. 2010 ; Vol. 41, No. 11. pp. 711-717.
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title = "Capacitance DNA bio-chips improved by new probe immobilization strategies",
abstract = "Label-free DNA detection plays a crucial role in developing point-of-care biochips. Capacitance detection is a promising technology for label-free detection. However, data published in literature often show evident time drift, large standard deviation, scattered data points, and poor reproducibility. To address these problems, mercapto-hexanol or similar alkanethiols are usually considered as blocking agents. The aim of the present paper is to investigate new blocking agents to further improve DNA probe surfaces. Data from AFM, SPR, florescence microscopy, and capacitance measurements are used to investigate new lipoate and ethylene-glycol molecules. The new surfaces offer further improvements in terms of diminished detection errors. Film structures are investigated at the nano-scale to justify the detection improvements in terms of probe surface quality. This study demonstrates the superiority of lipoate and ethylene-glycol molecules as blocking candidates when immobilizing molecular probes onto spot surfaces in label-free DNA biochip.",
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author = "Sandra Carrara and Andrea Cavallini and Yusuf Leblebici and {De Micheli}, Giovanni and Vijayender Bhalla and Francesco Valle and Bruno Samor{\`i} and Luca Benini and Bruno Ricc{\`o} and Inger Vikholm-Lundin and Tony Munter",
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Carrara, S, Cavallini, A, Leblebici, Y, De Micheli, G, Bhalla, V, Valle, F, Samorì, B, Benini, L, Riccò, B, Vikholm-Lundin, I & Munter, T 2010, 'Capacitance DNA bio-chips improved by new probe immobilization strategies', Microelectronics Journal, vol. 41, no. 11, pp. 711-717. https://doi.org/10.1016/j.mejo.2010.01.007

Capacitance DNA bio-chips improved by new probe immobilization strategies. / Carrara, Sandra (Corresponding Author); Cavallini, Andrea; Leblebici, Yusuf; De Micheli, Giovanni; Bhalla, Vijayender; Valle, Francesco; Samorì, Bruno; Benini, Luca; Riccò, Bruno; Vikholm-Lundin, Inger; Munter, Tony.

In: Microelectronics Journal, Vol. 41, No. 11, 2010, p. 711-717.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Capacitance DNA bio-chips improved by new probe immobilization strategies

AU - Carrara, Sandra

AU - Cavallini, Andrea

AU - Leblebici, Yusuf

AU - De Micheli, Giovanni

AU - Bhalla, Vijayender

AU - Valle, Francesco

AU - Samorì, Bruno

AU - Benini, Luca

AU - Riccò, Bruno

AU - Vikholm-Lundin, Inger

AU - Munter, Tony

PY - 2010

Y1 - 2010

N2 - Label-free DNA detection plays a crucial role in developing point-of-care biochips. Capacitance detection is a promising technology for label-free detection. However, data published in literature often show evident time drift, large standard deviation, scattered data points, and poor reproducibility. To address these problems, mercapto-hexanol or similar alkanethiols are usually considered as blocking agents. The aim of the present paper is to investigate new blocking agents to further improve DNA probe surfaces. Data from AFM, SPR, florescence microscopy, and capacitance measurements are used to investigate new lipoate and ethylene-glycol molecules. The new surfaces offer further improvements in terms of diminished detection errors. Film structures are investigated at the nano-scale to justify the detection improvements in terms of probe surface quality. This study demonstrates the superiority of lipoate and ethylene-glycol molecules as blocking candidates when immobilizing molecular probes onto spot surfaces in label-free DNA biochip.

AB - Label-free DNA detection plays a crucial role in developing point-of-care biochips. Capacitance detection is a promising technology for label-free detection. However, data published in literature often show evident time drift, large standard deviation, scattered data points, and poor reproducibility. To address these problems, mercapto-hexanol or similar alkanethiols are usually considered as blocking agents. The aim of the present paper is to investigate new blocking agents to further improve DNA probe surfaces. Data from AFM, SPR, florescence microscopy, and capacitance measurements are used to investigate new lipoate and ethylene-glycol molecules. The new surfaces offer further improvements in terms of diminished detection errors. Film structures are investigated at the nano-scale to justify the detection improvements in terms of probe surface quality. This study demonstrates the superiority of lipoate and ethylene-glycol molecules as blocking candidates when immobilizing molecular probes onto spot surfaces in label-free DNA biochip.

KW - Alkanethiols

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KW - Ethylene-glycol

KW - Lipoate-diethanolamines

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Carrara S, Cavallini A, Leblebici Y, De Micheli G, Bhalla V, Valle F et al. Capacitance DNA bio-chips improved by new probe immobilization strategies. Microelectronics Journal. 2010;41(11):711-717. https://doi.org/10.1016/j.mejo.2010.01.007