Capillary electrophoresis for the monitoring of carboxylic acid production by Gluconobacter oxydans

Heidi Turkia (Corresponding Author), Heli Sirén, Juha-Pekka Pitkänen, Marilyn Wiebe, Merja Penttilä

Research output: Contribution to journalArticleScientificpeer-review

24 Citations (Scopus)

Abstract

Determination of carboxylic acids in Gluconobacter oxydans fermentations of wheat straw hydrolyzate was carried out. This matrix is of complex composition containing carbohydrates, organic compounds (e.g., amino acids, toxins), and inorganic salts making the analysis challenging even with separation techniques. A method based on capillary electrophoresis with indirect UV detection was developed for the simultaneous quantification of 18 carboxylic acids. The background electrolyte solution of ammonia, 2,3-pyridinedicarboxylic acid, and Ca2+ and Mg2+ salts, containing myristyltrimethylammonium hydroxide as a dynamic capillary coating reagent, was validated for the robust and repeatable separation of the carboxylic acids. Intraday relative standard deviations in the optimized method were less than 1.6% for migration times and between 1.0% and 5.9% for peak area. Interday relative standard deviations were less than 5.0% for migration times and between 5.7% and 9.3% for peak area. With 11 nl injected, detection limits for the analytes were between 10 and 43 μmol/l. Detection limits ranged from 0.1 to 0.5 pmol at signal-to-noise ratio of 3. The results demonstrated that wheat straw hydrolyzate was a suitable substrate for G. oxydans with a product yield of 45% for the formation of xylonic acid from xylose and 96% for the formation of gluconic acid from glucose.

Original languageEnglish
Pages (from-to)1537-1542
JournalJournal of Chromatography A
Volume1217
Issue number9
DOIs
Publication statusPublished - 26 Feb 2010
MoE publication typeA1 Journal article-refereed

Fingerprint

Gluconobacter oxydans
Capillary electrophoresis
Capillary Electrophoresis
Carboxylic Acids
Straw
Triticum
Limit of Detection
Monitoring
Salts
Quinolinic Acid
Xylose
Signal-To-Noise Ratio
Organic compounds
Ammonia
Fermentation
Electrolytes
Signal to noise ratio
Carbohydrates
Amino Acids
Glucose

Keywords

  • Bioprocess monitoring
  • Capillary electrophoresis
  • Complex forming agents in electrolyte solution
  • Organic acids

Cite this

@article{77b61394ef254c13b8200862e8a636c3,
title = "Capillary electrophoresis for the monitoring of carboxylic acid production by Gluconobacter oxydans",
abstract = "Determination of carboxylic acids in Gluconobacter oxydans fermentations of wheat straw hydrolyzate was carried out. This matrix is of complex composition containing carbohydrates, organic compounds (e.g., amino acids, toxins), and inorganic salts making the analysis challenging even with separation techniques. A method based on capillary electrophoresis with indirect UV detection was developed for the simultaneous quantification of 18 carboxylic acids. The background electrolyte solution of ammonia, 2,3-pyridinedicarboxylic acid, and Ca2+ and Mg2+ salts, containing myristyltrimethylammonium hydroxide as a dynamic capillary coating reagent, was validated for the robust and repeatable separation of the carboxylic acids. Intraday relative standard deviations in the optimized method were less than 1.6{\%} for migration times and between 1.0{\%} and 5.9{\%} for peak area. Interday relative standard deviations were less than 5.0{\%} for migration times and between 5.7{\%} and 9.3{\%} for peak area. With 11 nl injected, detection limits for the analytes were between 10 and 43 μmol/l. Detection limits ranged from 0.1 to 0.5 pmol at signal-to-noise ratio of 3. The results demonstrated that wheat straw hydrolyzate was a suitable substrate for G. oxydans with a product yield of 45{\%} for the formation of xylonic acid from xylose and 96{\%} for the formation of gluconic acid from glucose.",
keywords = "Bioprocess monitoring, Capillary electrophoresis, Complex forming agents in electrolyte solution, Organic acids",
author = "Heidi Turkia and Heli Sir{\'e}n and Juha-Pekka Pitk{\"a}nen and Marilyn Wiebe and Merja Penttil{\"a}",
note = "CA2: TK402 CA2: TK406 CA2: TK400 ISI: CHEMISTRY, ANALYTICAL",
year = "2010",
month = "2",
day = "26",
doi = "10.1016/j.chroma.2009.12.075",
language = "English",
volume = "1217",
pages = "1537--1542",
journal = "Journal of Chromatography A",
issn = "0021-9673",
publisher = "Elsevier",
number = "9",

}

Capillary electrophoresis for the monitoring of carboxylic acid production by Gluconobacter oxydans. / Turkia, Heidi (Corresponding Author); Sirén, Heli; Pitkänen, Juha-Pekka; Wiebe, Marilyn; Penttilä, Merja.

In: Journal of Chromatography A, Vol. 1217, No. 9, 26.02.2010, p. 1537-1542.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Capillary electrophoresis for the monitoring of carboxylic acid production by Gluconobacter oxydans

AU - Turkia, Heidi

AU - Sirén, Heli

AU - Pitkänen, Juha-Pekka

AU - Wiebe, Marilyn

AU - Penttilä, Merja

N1 - CA2: TK402 CA2: TK406 CA2: TK400 ISI: CHEMISTRY, ANALYTICAL

PY - 2010/2/26

Y1 - 2010/2/26

N2 - Determination of carboxylic acids in Gluconobacter oxydans fermentations of wheat straw hydrolyzate was carried out. This matrix is of complex composition containing carbohydrates, organic compounds (e.g., amino acids, toxins), and inorganic salts making the analysis challenging even with separation techniques. A method based on capillary electrophoresis with indirect UV detection was developed for the simultaneous quantification of 18 carboxylic acids. The background electrolyte solution of ammonia, 2,3-pyridinedicarboxylic acid, and Ca2+ and Mg2+ salts, containing myristyltrimethylammonium hydroxide as a dynamic capillary coating reagent, was validated for the robust and repeatable separation of the carboxylic acids. Intraday relative standard deviations in the optimized method were less than 1.6% for migration times and between 1.0% and 5.9% for peak area. Interday relative standard deviations were less than 5.0% for migration times and between 5.7% and 9.3% for peak area. With 11 nl injected, detection limits for the analytes were between 10 and 43 μmol/l. Detection limits ranged from 0.1 to 0.5 pmol at signal-to-noise ratio of 3. The results demonstrated that wheat straw hydrolyzate was a suitable substrate for G. oxydans with a product yield of 45% for the formation of xylonic acid from xylose and 96% for the formation of gluconic acid from glucose.

AB - Determination of carboxylic acids in Gluconobacter oxydans fermentations of wheat straw hydrolyzate was carried out. This matrix is of complex composition containing carbohydrates, organic compounds (e.g., amino acids, toxins), and inorganic salts making the analysis challenging even with separation techniques. A method based on capillary electrophoresis with indirect UV detection was developed for the simultaneous quantification of 18 carboxylic acids. The background electrolyte solution of ammonia, 2,3-pyridinedicarboxylic acid, and Ca2+ and Mg2+ salts, containing myristyltrimethylammonium hydroxide as a dynamic capillary coating reagent, was validated for the robust and repeatable separation of the carboxylic acids. Intraday relative standard deviations in the optimized method were less than 1.6% for migration times and between 1.0% and 5.9% for peak area. Interday relative standard deviations were less than 5.0% for migration times and between 5.7% and 9.3% for peak area. With 11 nl injected, detection limits for the analytes were between 10 and 43 μmol/l. Detection limits ranged from 0.1 to 0.5 pmol at signal-to-noise ratio of 3. The results demonstrated that wheat straw hydrolyzate was a suitable substrate for G. oxydans with a product yield of 45% for the formation of xylonic acid from xylose and 96% for the formation of gluconic acid from glucose.

KW - Bioprocess monitoring

KW - Capillary electrophoresis

KW - Complex forming agents in electrolyte solution

KW - Organic acids

UR - http://www.scopus.com/inward/record.url?scp=75349093007&partnerID=8YFLogxK

U2 - 10.1016/j.chroma.2009.12.075

DO - 10.1016/j.chroma.2009.12.075

M3 - Article

C2 - 20074741

AN - SCOPUS:75349093007

VL - 1217

SP - 1537

EP - 1542

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 9

ER -