Capillary electrophoresis for the monitoring of carboxylic acid production by Gluconobacter oxydans

Heidi Turkia (Corresponding Author), Heli Sirén, Juha-Pekka Pitkänen, Marilyn Wiebe, Merja Penttilä

Research output: Contribution to journalArticleScientificpeer-review

28 Citations (Scopus)

Abstract

Determination of carboxylic acids in Gluconobacter oxydans fermentations of wheat straw hydrolyzate was carried out. This matrix is of complex composition containing carbohydrates, organic compounds (e.g., amino acids, toxins), and inorganic salts making the analysis challenging even with separation techniques. A method based on capillary electrophoresis with indirect UV detection was developed for the simultaneous quantification of 18 carboxylic acids. The background electrolyte solution of ammonia, 2,3-pyridinedicarboxylic acid, and Ca2+ and Mg2+ salts, containing myristyltrimethylammonium hydroxide as a dynamic capillary coating reagent, was validated for the robust and repeatable separation of the carboxylic acids. Intraday relative standard deviations in the optimized method were less than 1.6% for migration times and between 1.0% and 5.9% for peak area. Interday relative standard deviations were less than 5.0% for migration times and between 5.7% and 9.3% for peak area. With 11 nl injected, detection limits for the analytes were between 10 and 43 μmol/l. Detection limits ranged from 0.1 to 0.5 pmol at signal-to-noise ratio of 3. The results demonstrated that wheat straw hydrolyzate was a suitable substrate for G. oxydans with a product yield of 45% for the formation of xylonic acid from xylose and 96% for the formation of gluconic acid from glucose.

Original languageEnglish
Pages (from-to)1537-1542
JournalJournal of Chromatography A
Volume1217
Issue number9
DOIs
Publication statusPublished - 26 Feb 2010
MoE publication typeA1 Journal article-refereed

Keywords

  • Bioprocess monitoring
  • Capillary electrophoresis
  • Complex forming agents in electrolyte solution
  • Organic acids

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