Abstract
The study was focused on to determine inorganic anions
chloride, sulfate, nitrite and nitrate in biological
samples by capillary electrophoresis (CE) with an
indirect UV-detection technique. The results were used to
evaluate the correlation between the ion concentrations
in samples taken from patients having inflammation of
mammary papilla. The correlation of the anions was
supposed to explain the gravity of the illness.
Determination of plasma nitrite and nitrate are
increasingly used in clinical chemistry as markers for
the activity of nitric oxide synthesis and the production
of nitric oxide radicals. Nitrite and nitrate are the
most abundant circulating and excretory NO metabolites in
humans.
Capillary electrophoresis (CE) among HRGC-MS, HPLC and
spectrophotometry have been used for determination of NO
metabolites in clinical purposes. All of them, except CE,
need derivatization of the metabolites. Here we have
modified the technique for biological samples containing
chloride, sulfate, nitrite and nitrate without
complexation or derivatization. In CE the measurements
are based only on indirect UV-detection technique.
| Original language | English |
|---|---|
| Publication status | Published - 2003 |
| Event | VTT Life Science Day - Espoo, Finland Duration: 8 May 2003 → 8 May 2003 |
Seminar
| Seminar | VTT Life Science Day |
|---|---|
| Country/Territory | Finland |
| City | Espoo |
| Period | 8/05/03 → 8/05/03 |