Capillary electrophoresis of Cl-, SO42-, NO3-, NO2- in biological matrices

Heli Siren, Stella Rovio, Kaija Luomanperä, Teemu Työppönen, Pertti Vastamäki, Lotta Salminen

    Research output: Contribution to conferenceConference PosterScientific


    The study was focused on to determine inorganic anions chloride, sulfate, nitrite and nitrate in biological samples by capillary electrophoresis (CE) with an indirect UV-detection technique. The results were used to evaluate the correlation between the ion concentrations in samples taken from patients having inflammation of mammary papilla. The correlation of the anions was supposed to explain the gravity of the illness. Determination of plasma nitrite and nitrate are increasingly used in clinical chemistry as markers for the activity of nitric oxide synthesis and the production of nitric oxide radicals. Nitrite and nitrate are the most abundant circulating and excretory NO metabolites in humans. Capillary electrophoresis (CE) among HRGC-MS, HPLC and spectrophotometry have been used for determination of NO metabolites in clinical purposes. All of them, except CE, need derivatization of the metabolites. Here we have modified the technique for biological samples containing chloride, sulfate, nitrite and nitrate without complexation or derivatization. In CE the measurements are based only on indirect UV-detection technique.
    Original languageEnglish
    Publication statusPublished - 2003
    EventVTT Life Science Day - Espoo, Finland
    Duration: 8 May 20038 May 2003


    SeminarVTT Life Science Day


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