Capillary electrophoresis of Cl-, SO42-, NO3-, NO2- in biological matrices

Heli Siren, Stella Rovio, Kaija Luomanperä, Teemu Työppönen, Pertti Vastamäki, Lotta Salminen

Research output: Contribution to conferenceConference PosterScientific

Abstract

The study was focused on to determine inorganic anions chloride, sulfate, nitrite and nitrate in biological samples by capillary electrophoresis (CE) with an indirect UV-detection technique. The results were used to evaluate the correlation between the ion concentrations in samples taken from patients having inflammation of mammary papilla. The correlation of the anions was supposed to explain the gravity of the illness. Determination of plasma nitrite and nitrate are increasingly used in clinical chemistry as markers for the activity of nitric oxide synthesis and the production of nitric oxide radicals. Nitrite and nitrate are the most abundant circulating and excretory NO metabolites in humans. Capillary electrophoresis (CE) among HRGC-MS, HPLC and spectrophotometry have been used for determination of NO metabolites in clinical purposes. All of them, except CE, need derivatization of the metabolites. Here we have modified the technique for biological samples containing chloride, sulfate, nitrite and nitrate without complexation or derivatization. In CE the measurements are based only on indirect UV-detection technique.
Original languageEnglish
Publication statusPublished - 2003
EventVTT Life Science Day - Espoo, Finland
Duration: 8 May 20038 May 2003

Seminar

SeminarVTT Life Science Day
CountryFinland
CityEspoo
Period8/05/038/05/03

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Capillary electrophoresis
Nitrites
Nitrates
Metabolites
Sulfates
Anions
Chlorides
Nitric Oxide
Spectrophotometry
Complexation
Gravitation
Ions
Plasmas

Cite this

Siren, H., Rovio, S., Luomanperä, K., Työppönen, T., Vastamäki, P., & Salminen, L. (2003). Capillary electrophoresis of Cl-, SO42-, NO3-, NO2- in biological matrices. Poster session presented at VTT Life Science Day, Espoo, Finland.
Siren, Heli ; Rovio, Stella ; Luomanperä, Kaija ; Työppönen, Teemu ; Vastamäki, Pertti ; Salminen, Lotta. / Capillary electrophoresis of Cl-, SO42-, NO3-, NO2- in biological matrices. Poster session presented at VTT Life Science Day, Espoo, Finland.
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abstract = "The study was focused on to determine inorganic anions chloride, sulfate, nitrite and nitrate in biological samples by capillary electrophoresis (CE) with an indirect UV-detection technique. The results were used to evaluate the correlation between the ion concentrations in samples taken from patients having inflammation of mammary papilla. The correlation of the anions was supposed to explain the gravity of the illness. Determination of plasma nitrite and nitrate are increasingly used in clinical chemistry as markers for the activity of nitric oxide synthesis and the production of nitric oxide radicals. Nitrite and nitrate are the most abundant circulating and excretory NO metabolites in humans. Capillary electrophoresis (CE) among HRGC-MS, HPLC and spectrophotometry have been used for determination of NO metabolites in clinical purposes. All of them, except CE, need derivatization of the metabolites. Here we have modified the technique for biological samples containing chloride, sulfate, nitrite and nitrate without complexation or derivatization. In CE the measurements are based only on indirect UV-detection technique.",
author = "Heli Siren and Stella Rovio and Kaija Luomanper{\"a} and Teemu Ty{\"o}pp{\"o}nen and Pertti Vastam{\"a}ki and Lotta Salminen",
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Siren, H, Rovio, S, Luomanperä, K, Työppönen, T, Vastamäki, P & Salminen, L 2003, 'Capillary electrophoresis of Cl-, SO42-, NO3-, NO2- in biological matrices' VTT Life Science Day, Espoo, Finland, 8/05/03 - 8/05/03, .

Capillary electrophoresis of Cl-, SO42-, NO3-, NO2- in biological matrices. / Siren, Heli; Rovio, Stella; Luomanperä, Kaija; Työppönen, Teemu; Vastamäki, Pertti; Salminen, Lotta.

2003. Poster session presented at VTT Life Science Day, Espoo, Finland.

Research output: Contribution to conferenceConference PosterScientific

TY - CONF

T1 - Capillary electrophoresis of Cl-, SO42-, NO3-, NO2- in biological matrices

AU - Siren, Heli

AU - Rovio, Stella

AU - Luomanperä, Kaija

AU - Työppönen, Teemu

AU - Vastamäki, Pertti

AU - Salminen, Lotta

PY - 2003

Y1 - 2003

N2 - The study was focused on to determine inorganic anions chloride, sulfate, nitrite and nitrate in biological samples by capillary electrophoresis (CE) with an indirect UV-detection technique. The results were used to evaluate the correlation between the ion concentrations in samples taken from patients having inflammation of mammary papilla. The correlation of the anions was supposed to explain the gravity of the illness. Determination of plasma nitrite and nitrate are increasingly used in clinical chemistry as markers for the activity of nitric oxide synthesis and the production of nitric oxide radicals. Nitrite and nitrate are the most abundant circulating and excretory NO metabolites in humans. Capillary electrophoresis (CE) among HRGC-MS, HPLC and spectrophotometry have been used for determination of NO metabolites in clinical purposes. All of them, except CE, need derivatization of the metabolites. Here we have modified the technique for biological samples containing chloride, sulfate, nitrite and nitrate without complexation or derivatization. In CE the measurements are based only on indirect UV-detection technique.

AB - The study was focused on to determine inorganic anions chloride, sulfate, nitrite and nitrate in biological samples by capillary electrophoresis (CE) with an indirect UV-detection technique. The results were used to evaluate the correlation between the ion concentrations in samples taken from patients having inflammation of mammary papilla. The correlation of the anions was supposed to explain the gravity of the illness. Determination of plasma nitrite and nitrate are increasingly used in clinical chemistry as markers for the activity of nitric oxide synthesis and the production of nitric oxide radicals. Nitrite and nitrate are the most abundant circulating and excretory NO metabolites in humans. Capillary electrophoresis (CE) among HRGC-MS, HPLC and spectrophotometry have been used for determination of NO metabolites in clinical purposes. All of them, except CE, need derivatization of the metabolites. Here we have modified the technique for biological samples containing chloride, sulfate, nitrite and nitrate without complexation or derivatization. In CE the measurements are based only on indirect UV-detection technique.

M3 - Conference Poster

ER -

Siren H, Rovio S, Luomanperä K, Työppönen T, Vastamäki P, Salminen L. Capillary electrophoresis of Cl-, SO42-, NO3-, NO2- in biological matrices. 2003. Poster session presented at VTT Life Science Day, Espoo, Finland.