Capillary electrophoresis with UV detection and mass spectrometry in method development for profiling metabolites of steroid hormone metabolism

Heli Sirén (Corresponding Author), Tuulikki Seppänen-Laakso, Matej Oresic

Research output: Contribution to journalArticleScientificpeer-review

38 Citations (Scopus)

Abstract

The aim of this study was to develop a method for comprehensive profiling of metabolites involved in mammalian steroid metabolism. The study was performed using the partial filling micellar electrokinetic chromatography (PF-MEKC) technique for determination of endogenous low-hydrophilic steroids. The detection techniques in capillary electrophoresis were UV absorption and electrospray mass spectrometry (ESI-MS). Thirteen steroids were included in the method development, and the selected were metabolites involved in major pathways of steroid biosynthesis. Although only eight of them could be separated and detected with UV, they could be identified by ESI-MS using selected ion monitoring (SIM) technique. Tandem MS spectra were also collected. UV detection was more sensitive than MS due to better separation of compounds and the selective signal sensitivity. The lowest limits of detection were 10–100 ng/mL for cortisone, corticosterone, hydrocortisone and testosterone. The other steroids could be detected at 500–1000 ng/mL. The identification of cortisone, corticosterone, hydrocortisone, estrogen and testosterone were made in patient urine samples and their concentrations were 1–40 μg/L.
Original languageEnglish
Pages (from-to)375-382
Number of pages8
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume871
Issue number2
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Fingerprint

Steroid hormones
Capillary electrophoresis
Capillary Electrophoresis
Metabolites
Metabolism
Mass spectrometry
Mass Spectrometry
Steroids
Hormones
Cortisone
Corticosterone
Hydrocortisone
Testosterone
Electrospray Ionization Mass Spectrometry
Biosynthesis
Chromatography
Limit of Detection
Estrogens
Urine
Ions

Keywords

  • Steroids
  • Partial filling
  • Micellar electrokinetic capillary electrophoresis
  • Mass spectrometry
  • Urine

Cite this

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title = "Capillary electrophoresis with UV detection and mass spectrometry in method development for profiling metabolites of steroid hormone metabolism",
abstract = "The aim of this study was to develop a method for comprehensive profiling of metabolites involved in mammalian steroid metabolism. The study was performed using the partial filling micellar electrokinetic chromatography (PF-MEKC) technique for determination of endogenous low-hydrophilic steroids. The detection techniques in capillary electrophoresis were UV absorption and electrospray mass spectrometry (ESI-MS). Thirteen steroids were included in the method development, and the selected were metabolites involved in major pathways of steroid biosynthesis. Although only eight of them could be separated and detected with UV, they could be identified by ESI-MS using selected ion monitoring (SIM) technique. Tandem MS spectra were also collected. UV detection was more sensitive than MS due to better separation of compounds and the selective signal sensitivity. The lowest limits of detection were 10–100 ng/mL for cortisone, corticosterone, hydrocortisone and testosterone. The other steroids could be detected at 500–1000 ng/mL. The identification of cortisone, corticosterone, hydrocortisone, estrogen and testosterone were made in patient urine samples and their concentrations were 1–40 μg/L.",
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Capillary electrophoresis with UV detection and mass spectrometry in method development for profiling metabolites of steroid hormone metabolism. / Sirén, Heli (Corresponding Author); Seppänen-Laakso, Tuulikki; Oresic, Matej.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 871, No. 2, 2008, p. 375-382.

Research output: Contribution to journalArticleScientificpeer-review

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