Capillary electrophoretic separation of dicarboxylic acids in atmospheric aerosol particles

Heidi Adler, Heli Siren, Markku Kulmala, Marja-Liisa Riekkola (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

16 Citations (Scopus)

Abstract

2,3-Pyrazinedicarboxylic acid (PZDA), 2,6-pyridinedicarboxylic acid (PDA) and 2,3-pyridinedicarboxylic acid (QUIN) solutions were studied as background electrolytes (BGEs) in the capillary electrophoretic analysis of dicarboxylic acids in aerosol particles with indirect UV detection. The BGEs were selected on the basis of similarity in structure with the analytes so that mobilities would be compatible. Optimised pH values for PZDA, PDA and QUIN solutions were 10.6, 11.0 and 10.2, respectively. Myristyltrimethylammonium hydroxide and myristyltrimethylammonium bromide were added to reverse the electroosmotic flow in the solutions in the direction of anode to enable fast anion detection. Separation was obtained for nine dicarboxylic acids (C2–C10) differing in the number of CH2 groups in their skeleton. The electrophoretic mobilities were determined to lie in the range 3.0×10−4–7.0×10−4 cm2 V−1 s−1. The relative standard deviations (RSDs) for the absolute migration times of the analytes were mostly less than 0.5% (n=6) in PZDA solution. In PDA solution the within-day and day-to-day RSD values for migration were less than 1% and between 2 and 4%, respectively. Peak heights and areas mostly deviated between 1 and 15% in both PZDA and PDA solutions. Detection limits ranged between 1 and 5 mg/l. Methods were applied to the analysis of dicarboxylic acids isolated from aerosol particles.
Original languageEnglish
Pages (from-to)133-141
Number of pages9
JournalJournal of Chromatography A
Volume990
Issue number1-2
DOIs
Publication statusPublished - 2003
MoE publication typeA1 Journal article-refereed
Event13th International Symposium on Capillary Electroseparation Techniques - Helsinki, Finland
Duration: 1 Sep 20024 Sep 2002

Fingerprint

Atmospheric aerosols
Dicarboxylic Acids
Aerosols
Particles (particulate matter)
Electrolytes
Acids
Electroosmosis
Quinolinic Acid
Electrophoretic mobility
Bromides
Skeleton
Anions
Limit of Detection
Anodes
Electrodes
2,3-pyrazinedicarboxylic acid

Keywords

  • aerosols
  • air analysis
  • background electrolyte composition
  • environmental analysis
  • dicarboxylic acids

Cite this

Adler, Heidi ; Siren, Heli ; Kulmala, Markku ; Riekkola, Marja-Liisa. / Capillary electrophoretic separation of dicarboxylic acids in atmospheric aerosol particles. In: Journal of Chromatography A. 2003 ; Vol. 990, No. 1-2. pp. 133-141.
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Capillary electrophoretic separation of dicarboxylic acids in atmospheric aerosol particles. / Adler, Heidi; Siren, Heli; Kulmala, Markku; Riekkola, Marja-Liisa (Corresponding Author).

In: Journal of Chromatography A, Vol. 990, No. 1-2, 2003, p. 133-141.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Capillary electrophoretic separation of dicarboxylic acids in atmospheric aerosol particles

AU - Adler, Heidi

AU - Siren, Heli

AU - Kulmala, Markku

AU - Riekkola, Marja-Liisa

PY - 2003

Y1 - 2003

N2 - 2,3-Pyrazinedicarboxylic acid (PZDA), 2,6-pyridinedicarboxylic acid (PDA) and 2,3-pyridinedicarboxylic acid (QUIN) solutions were studied as background electrolytes (BGEs) in the capillary electrophoretic analysis of dicarboxylic acids in aerosol particles with indirect UV detection. The BGEs were selected on the basis of similarity in structure with the analytes so that mobilities would be compatible. Optimised pH values for PZDA, PDA and QUIN solutions were 10.6, 11.0 and 10.2, respectively. Myristyltrimethylammonium hydroxide and myristyltrimethylammonium bromide were added to reverse the electroosmotic flow in the solutions in the direction of anode to enable fast anion detection. Separation was obtained for nine dicarboxylic acids (C2–C10) differing in the number of CH2 groups in their skeleton. The electrophoretic mobilities were determined to lie in the range 3.0×10−4–7.0×10−4 cm2 V−1 s−1. The relative standard deviations (RSDs) for the absolute migration times of the analytes were mostly less than 0.5% (n=6) in PZDA solution. In PDA solution the within-day and day-to-day RSD values for migration were less than 1% and between 2 and 4%, respectively. Peak heights and areas mostly deviated between 1 and 15% in both PZDA and PDA solutions. Detection limits ranged between 1 and 5 mg/l. Methods were applied to the analysis of dicarboxylic acids isolated from aerosol particles.

AB - 2,3-Pyrazinedicarboxylic acid (PZDA), 2,6-pyridinedicarboxylic acid (PDA) and 2,3-pyridinedicarboxylic acid (QUIN) solutions were studied as background electrolytes (BGEs) in the capillary electrophoretic analysis of dicarboxylic acids in aerosol particles with indirect UV detection. The BGEs were selected on the basis of similarity in structure with the analytes so that mobilities would be compatible. Optimised pH values for PZDA, PDA and QUIN solutions were 10.6, 11.0 and 10.2, respectively. Myristyltrimethylammonium hydroxide and myristyltrimethylammonium bromide were added to reverse the electroosmotic flow in the solutions in the direction of anode to enable fast anion detection. Separation was obtained for nine dicarboxylic acids (C2–C10) differing in the number of CH2 groups in their skeleton. The electrophoretic mobilities were determined to lie in the range 3.0×10−4–7.0×10−4 cm2 V−1 s−1. The relative standard deviations (RSDs) for the absolute migration times of the analytes were mostly less than 0.5% (n=6) in PZDA solution. In PDA solution the within-day and day-to-day RSD values for migration were less than 1% and between 2 and 4%, respectively. Peak heights and areas mostly deviated between 1 and 15% in both PZDA and PDA solutions. Detection limits ranged between 1 and 5 mg/l. Methods were applied to the analysis of dicarboxylic acids isolated from aerosol particles.

KW - aerosols

KW - air analysis

KW - background electrolyte composition

KW - environmental analysis

KW - dicarboxylic acids

U2 - 10.1016/S0021-9673(03)00051-7

DO - 10.1016/S0021-9673(03)00051-7

M3 - Article

VL - 990

SP - 133

EP - 141

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 1-2

ER -