Capillary zone electrophoresis of cationic and anionic drygs in methanol

Heli Sirén, Ruth Kuldvee, Tiina Karla, Tiina Ekström, Marja-Liisa Riekkola (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

13 Citations (Scopus)

Abstract

The actual mobilities and dissociation constants of acidic and basic pharmaceuticals were determined in methanol. Actual mobilities were derived from the dependence of the effective mobilities of the analytes on the pH of the methanolic background electrolyte solution (pHMeOH). The pKa values of the pharmaceuticals in methanol (pKa,MeOH) were calculated by non-linear curve fitting to the measured mobility values. It was found that the shift in pKa value (when compounds were transferred from water to methanol) increased with the acidity of the analyte. The average pKa shift for compounds exhibiting acidic properties in water was ca. 5.5 units, and the shift for basic compounds about 2 units. As was shown for a mixture of β-blockers, the calculated actual mobilities and pKa values can be utilised in the optimisation of pH conditions for separation. The practical value of the method was illustrated by the analysis of urine samples.
Original languageEnglish
Pages (from-to)89 - 97
Number of pages9
JournalJournal of Chromatography A
Volume1068
Issue number1
DOIs
Publication statusPublished - 2005
MoE publication typeA1 Journal article-refereed

Fingerprint

Capillary Electrophoresis
Electrophoresis
Methanol
Water
Curve fitting
Acidity
Pharmaceutical Preparations
Electrolytes
Urine

Keywords

  • nonaqueous capillary electrophoresis
  • methanol
  • b-blockers
  • catecholamine metabolites
  • metabolites
  • dissociation constants
  • urine

Cite this

Sirén, H., Kuldvee, R., Karla, T., Ekström, T., & Riekkola, M-L. (2005). Capillary zone electrophoresis of cationic and anionic drygs in methanol. Journal of Chromatography A, 1068(1), 89 - 97. https://doi.org/10.1016/j.chroma.2004.11.082
Sirén, Heli ; Kuldvee, Ruth ; Karla, Tiina ; Ekström, Tiina ; Riekkola, Marja-Liisa. / Capillary zone electrophoresis of cationic and anionic drygs in methanol. In: Journal of Chromatography A. 2005 ; Vol. 1068, No. 1. pp. 89 - 97.
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Sirén, H, Kuldvee, R, Karla, T, Ekström, T & Riekkola, M-L 2005, 'Capillary zone electrophoresis of cationic and anionic drygs in methanol', Journal of Chromatography A, vol. 1068, no. 1, pp. 89 - 97. https://doi.org/10.1016/j.chroma.2004.11.082

Capillary zone electrophoresis of cationic and anionic drygs in methanol. / Sirén, Heli; Kuldvee, Ruth; Karla, Tiina; Ekström, Tiina; Riekkola, Marja-Liisa (Corresponding Author).

In: Journal of Chromatography A, Vol. 1068, No. 1, 2005, p. 89 - 97.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Capillary zone electrophoresis of cationic and anionic drygs in methanol

AU - Sirén, Heli

AU - Kuldvee, Ruth

AU - Karla, Tiina

AU - Ekström, Tiina

AU - Riekkola, Marja-Liisa

N1 - Project code: C2SU02062

PY - 2005

Y1 - 2005

N2 - The actual mobilities and dissociation constants of acidic and basic pharmaceuticals were determined in methanol. Actual mobilities were derived from the dependence of the effective mobilities of the analytes on the pH of the methanolic background electrolyte solution (pHMeOH). The pKa values of the pharmaceuticals in methanol (pKa,MeOH) were calculated by non-linear curve fitting to the measured mobility values. It was found that the shift in pKa value (when compounds were transferred from water to methanol) increased with the acidity of the analyte. The average pKa shift for compounds exhibiting acidic properties in water was ca. 5.5 units, and the shift for basic compounds about 2 units. As was shown for a mixture of β-blockers, the calculated actual mobilities and pKa values can be utilised in the optimisation of pH conditions for separation. The practical value of the method was illustrated by the analysis of urine samples.

AB - The actual mobilities and dissociation constants of acidic and basic pharmaceuticals were determined in methanol. Actual mobilities were derived from the dependence of the effective mobilities of the analytes on the pH of the methanolic background electrolyte solution (pHMeOH). The pKa values of the pharmaceuticals in methanol (pKa,MeOH) were calculated by non-linear curve fitting to the measured mobility values. It was found that the shift in pKa value (when compounds were transferred from water to methanol) increased with the acidity of the analyte. The average pKa shift for compounds exhibiting acidic properties in water was ca. 5.5 units, and the shift for basic compounds about 2 units. As was shown for a mixture of β-blockers, the calculated actual mobilities and pKa values can be utilised in the optimisation of pH conditions for separation. The practical value of the method was illustrated by the analysis of urine samples.

KW - nonaqueous capillary electrophoresis

KW - methanol

KW - b-blockers

KW - catecholamine metabolites

KW - metabolites

KW - dissociation constants

KW - urine

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