Carbohydrate analysis of plant materials with uronic acid-containing polysaccharides

A comparison between different hydrolysis and subsequent chromatographic analytical techniques

Stefan Willför (Corresponding Author), Andrey Pranovich, Tarja Tamminen, Juergen Puls, Christiane Laine, Anna Suurnäkki, Bodo Saake, Kati Uotila, Helena Simolin, Jarl Hemming, Bjarne Holmbom

Research output: Contribution to journalArticleScientificpeer-review

169 Citations (Scopus)

Abstract

Acid hydrolysis, acid methanolysis, and enzymatic hydrolysis were compared for depolymerization of five different plant materials containing uronic acids. The analyzed plant materials were oat spelt, wheat straw, spruce thermomechanical pulp, aspen stemwood, and totally chlorine-free (TCF) bleached hardwood kraft pulp. Furthermore, GC (using both HP-1 and HP-5 capillary columns and FID and MSD detectors), HPAEC-PAD, and HPAEC-Borate techniques were compared for subsequent analysis of the released monosaccharides. It was shown that acid methanolysis combined with GC analysis is a convenient method for obtaining the sugar unit composition and amount of non-crystalline polysaccharides in different plant materials. The methanolysis method was generally superior to the hydrolysis method for xylan- and uronic acid-containing samples. However, acid and enzymatic hydrolysis showed the highest recoveries for bleached chemical pulp samples. Acid hydrolysis is also required for crystalline polysaccharides, but the strong acid conditions evidently lead to degradation of labile sugars. The plant methanolysates were not suitable as such for analysis on an HPAEC-PAD system. For analysis of the total amount of sugar units, hence including cellulose, other non-crystalline hemicelluloses, and pectins, a combination of the methanolysis and hydrolysis methods is recommended.
Original languageEnglish
Pages (from-to)571-580
Number of pages10
JournalIndustrial Crops and Products
Volume29
Issue number2-3
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

uronic acids
methanolysis
analytical methods
chromatography
polysaccharides
hydrolysis
carbohydrates
acid hydrolysis
enzymatic hydrolysis
sugars
acids
chemical pulp
Triticum aestivum subsp. spelta
kraft pulp
methodology
borates
stemwood
depolymerization
xylan
monosaccharides

Keywords

  • Acid methanolysis
  • Acid hydrolysis
  • Enzymatic hydrolysis
  • GC-FID
  • GC-MS
  • HPAEC-PAD
  • HPAEC-Borate

Cite this

Willför, Stefan ; Pranovich, Andrey ; Tamminen, Tarja ; Puls, Juergen ; Laine, Christiane ; Suurnäkki, Anna ; Saake, Bodo ; Uotila, Kati ; Simolin, Helena ; Hemming, Jarl ; Holmbom, Bjarne. / Carbohydrate analysis of plant materials with uronic acid-containing polysaccharides : A comparison between different hydrolysis and subsequent chromatographic analytical techniques. In: Industrial Crops and Products. 2009 ; Vol. 29, No. 2-3. pp. 571-580.
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Carbohydrate analysis of plant materials with uronic acid-containing polysaccharides : A comparison between different hydrolysis and subsequent chromatographic analytical techniques. / Willför, Stefan (Corresponding Author); Pranovich, Andrey; Tamminen, Tarja; Puls, Juergen; Laine, Christiane; Suurnäkki, Anna; Saake, Bodo; Uotila, Kati; Simolin, Helena; Hemming, Jarl; Holmbom, Bjarne.

In: Industrial Crops and Products, Vol. 29, No. 2-3, 2009, p. 571-580.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Carbohydrate analysis of plant materials with uronic acid-containing polysaccharides

T2 - A comparison between different hydrolysis and subsequent chromatographic analytical techniques

AU - Willför, Stefan

AU - Pranovich, Andrey

AU - Tamminen, Tarja

AU - Puls, Juergen

AU - Laine, Christiane

AU - Suurnäkki, Anna

AU - Saake, Bodo

AU - Uotila, Kati

AU - Simolin, Helena

AU - Hemming, Jarl

AU - Holmbom, Bjarne

PY - 2009

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N2 - Acid hydrolysis, acid methanolysis, and enzymatic hydrolysis were compared for depolymerization of five different plant materials containing uronic acids. The analyzed plant materials were oat spelt, wheat straw, spruce thermomechanical pulp, aspen stemwood, and totally chlorine-free (TCF) bleached hardwood kraft pulp. Furthermore, GC (using both HP-1 and HP-5 capillary columns and FID and MSD detectors), HPAEC-PAD, and HPAEC-Borate techniques were compared for subsequent analysis of the released monosaccharides. It was shown that acid methanolysis combined with GC analysis is a convenient method for obtaining the sugar unit composition and amount of non-crystalline polysaccharides in different plant materials. The methanolysis method was generally superior to the hydrolysis method for xylan- and uronic acid-containing samples. However, acid and enzymatic hydrolysis showed the highest recoveries for bleached chemical pulp samples. Acid hydrolysis is also required for crystalline polysaccharides, but the strong acid conditions evidently lead to degradation of labile sugars. The plant methanolysates were not suitable as such for analysis on an HPAEC-PAD system. For analysis of the total amount of sugar units, hence including cellulose, other non-crystalline hemicelluloses, and pectins, a combination of the methanolysis and hydrolysis methods is recommended.

AB - Acid hydrolysis, acid methanolysis, and enzymatic hydrolysis were compared for depolymerization of five different plant materials containing uronic acids. The analyzed plant materials were oat spelt, wheat straw, spruce thermomechanical pulp, aspen stemwood, and totally chlorine-free (TCF) bleached hardwood kraft pulp. Furthermore, GC (using both HP-1 and HP-5 capillary columns and FID and MSD detectors), HPAEC-PAD, and HPAEC-Borate techniques were compared for subsequent analysis of the released monosaccharides. It was shown that acid methanolysis combined with GC analysis is a convenient method for obtaining the sugar unit composition and amount of non-crystalline polysaccharides in different plant materials. The methanolysis method was generally superior to the hydrolysis method for xylan- and uronic acid-containing samples. However, acid and enzymatic hydrolysis showed the highest recoveries for bleached chemical pulp samples. Acid hydrolysis is also required for crystalline polysaccharides, but the strong acid conditions evidently lead to degradation of labile sugars. The plant methanolysates were not suitable as such for analysis on an HPAEC-PAD system. For analysis of the total amount of sugar units, hence including cellulose, other non-crystalline hemicelluloses, and pectins, a combination of the methanolysis and hydrolysis methods is recommended.

KW - Acid methanolysis

KW - Acid hydrolysis

KW - Enzymatic hydrolysis

KW - GC-FID

KW - GC-MS

KW - HPAEC-PAD

KW - HPAEC-Borate

U2 - 10.1016/j.indcrop.2008.11.003

DO - 10.1016/j.indcrop.2008.11.003

M3 - Article

VL - 29

SP - 571

EP - 580

JO - Industrial Crops and Products

JF - Industrial Crops and Products

SN - 0926-6690

IS - 2-3

ER -