TY - JOUR
T1 - Carbohydrate analysis of plant materials with uronic acid-containing polysaccharides
T2 - A comparison between different hydrolysis and subsequent chromatographic analytical techniques
AU - Willför, Stefan
AU - Pranovich, Andrey
AU - Tamminen, Tarja
AU - Puls, Juergen
AU - Laine, Christiane
AU - Suurnäkki, Anna
AU - Saake, Bodo
AU - Uotila, Kati
AU - Simolin, Helena
AU - Hemming, Jarl
AU - Holmbom, Bjarne
PY - 2009
Y1 - 2009
N2 - Acid hydrolysis, acid methanolysis, and enzymatic hydrolysis were compared for depolymerization of five different plant materials containing uronic acids. The analyzed plant materials were oat spelt, wheat straw, spruce thermomechanical pulp, aspen stemwood, and totally chlorine-free (TCF) bleached hardwood kraft pulp. Furthermore, GC (using both HP-1 and HP-5 capillary columns and FID and MSD detectors), HPAEC-PAD, and HPAEC-Borate techniques were compared for subsequent analysis of the released monosaccharides. It was shown that acid methanolysis combined with GC analysis is a convenient method for obtaining the sugar unit composition and amount of non-crystalline polysaccharides in different plant materials. The methanolysis method was generally superior to the hydrolysis method for xylan- and uronic acid-containing samples. However, acid and enzymatic hydrolysis showed the highest recoveries for bleached chemical pulp samples. Acid hydrolysis is also required for crystalline polysaccharides, but the strong acid conditions evidently lead to degradation of labile sugars. The plant methanolysates were not suitable as such for analysis on an HPAEC-PAD system. For analysis of the total amount of sugar units, hence including cellulose, other non-crystalline hemicelluloses, and pectins, a combination of the methanolysis and hydrolysis methods is recommended.
AB - Acid hydrolysis, acid methanolysis, and enzymatic hydrolysis were compared for depolymerization of five different plant materials containing uronic acids. The analyzed plant materials were oat spelt, wheat straw, spruce thermomechanical pulp, aspen stemwood, and totally chlorine-free (TCF) bleached hardwood kraft pulp. Furthermore, GC (using both HP-1 and HP-5 capillary columns and FID and MSD detectors), HPAEC-PAD, and HPAEC-Borate techniques were compared for subsequent analysis of the released monosaccharides. It was shown that acid methanolysis combined with GC analysis is a convenient method for obtaining the sugar unit composition and amount of non-crystalline polysaccharides in different plant materials. The methanolysis method was generally superior to the hydrolysis method for xylan- and uronic acid-containing samples. However, acid and enzymatic hydrolysis showed the highest recoveries for bleached chemical pulp samples. Acid hydrolysis is also required for crystalline polysaccharides, but the strong acid conditions evidently lead to degradation of labile sugars. The plant methanolysates were not suitable as such for analysis on an HPAEC-PAD system. For analysis of the total amount of sugar units, hence including cellulose, other non-crystalline hemicelluloses, and pectins, a combination of the methanolysis and hydrolysis methods is recommended.
KW - Acid methanolysis
KW - Acid hydrolysis
KW - Enzymatic hydrolysis
KW - GC-FID
KW - GC-MS
KW - HPAEC-PAD
KW - HPAEC-Borate
U2 - 10.1016/j.indcrop.2008.11.003
DO - 10.1016/j.indcrop.2008.11.003
M3 - Article
SN - 0926-6690
VL - 29
SP - 571
EP - 580
JO - Industrial Crops and Products
JF - Industrial Crops and Products
IS - 2-3
ER -