Carbon 13-Metabolic Flux Analysis derived constraint-based metabolic modelling of Clostridium acetobutylicum in stressed chemostat conditions

Janne Wallenius (Corresponding Author), Hannu Maaheimo, Tero Eerikäinen

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

The metabolism of butanol producing bacteria Clostridium acetobutylicum was studied in chemostat with glucose limited conditions, butanol stimulus, and as a reference cultivation. COnstraint-Based Reconstruction and Analysis (COBRA) was applied using additional constraints from 13C Metabolic Flux Analysis (13C-MFA) and experimental measurement results. A model consisting of 451 metabolites and 604 reactions was utilized in flux balance analysis (FBA). The stringency of the flux spaces considering different optimization objectives, i.e. growth rate maximization, ATP maintenance, and NADH/NADPH formation, for flux variance analysis (FVA) was studied in the different modelled conditions. Also a previously uncharacterized exopolysaccharide (EPS) produced by C. acetobutylicum was characterized on monosaccharide level. The major monosaccharide components of the EPS were 40 n-% rhamnose, 34 n-% glucose, 13 n-% mannose, 10 n-% galactose, and 2 n-% arabinose. The EPS was studied to have butanol adsorbing property, 70 (butanol) mg (EPS) g-1 at 37 °C.
Original languageEnglish
Pages (from-to)378-386
JournalBioresource Technology
Volume219
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fingerprint

Chemostats
Clostridium
Butanols
chemostat
Butenes
carbon isotope
monosaccharide
Carbon
Fluxes
Monosaccharides
glucose
modeling
Glucose
variance analysis
Rhamnose
Arabinose
Adenosinetriphosphate
metabolite
Mannose
Metabolites

Keywords

  • 13C metabolic flux analysis
  • butanol stress
  • C. acetobutylicum
  • COBRA
  • exopolysaccharide

Cite this

@article{731e7df005814f88b5fcce79dfb4de9c,
title = "Carbon 13-Metabolic Flux Analysis derived constraint-based metabolic modelling of Clostridium acetobutylicum in stressed chemostat conditions",
abstract = "The metabolism of butanol producing bacteria Clostridium acetobutylicum was studied in chemostat with glucose limited conditions, butanol stimulus, and as a reference cultivation. COnstraint-Based Reconstruction and Analysis (COBRA) was applied using additional constraints from 13C Metabolic Flux Analysis (13C-MFA) and experimental measurement results. A model consisting of 451 metabolites and 604 reactions was utilized in flux balance analysis (FBA). The stringency of the flux spaces considering different optimization objectives, i.e. growth rate maximization, ATP maintenance, and NADH/NADPH formation, for flux variance analysis (FVA) was studied in the different modelled conditions. Also a previously uncharacterized exopolysaccharide (EPS) produced by C. acetobutylicum was characterized on monosaccharide level. The major monosaccharide components of the EPS were 40 n-{\%} rhamnose, 34 n-{\%} glucose, 13 n-{\%} mannose, 10 n-{\%} galactose, and 2 n-{\%} arabinose. The EPS was studied to have butanol adsorbing property, 70 (butanol) mg (EPS) g-1 at 37 °C.",
keywords = "13C metabolic flux analysis, butanol stress, C. acetobutylicum, COBRA, exopolysaccharide",
author = "Janne Wallenius and Hannu Maaheimo and Tero Eerik{\"a}inen",
year = "2016",
doi = "10.1016/j.biortech.2016.07.137",
language = "English",
volume = "219",
pages = "378--386",
journal = "Bioresource Technology",
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}

Carbon 13-Metabolic Flux Analysis derived constraint-based metabolic modelling of Clostridium acetobutylicum in stressed chemostat conditions. / Wallenius, Janne (Corresponding Author); Maaheimo, Hannu; Eerikäinen, Tero.

In: Bioresource Technology, Vol. 219, 2016, p. 378-386.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Carbon 13-Metabolic Flux Analysis derived constraint-based metabolic modelling of Clostridium acetobutylicum in stressed chemostat conditions

AU - Wallenius, Janne

AU - Maaheimo, Hannu

AU - Eerikäinen, Tero

PY - 2016

Y1 - 2016

N2 - The metabolism of butanol producing bacteria Clostridium acetobutylicum was studied in chemostat with glucose limited conditions, butanol stimulus, and as a reference cultivation. COnstraint-Based Reconstruction and Analysis (COBRA) was applied using additional constraints from 13C Metabolic Flux Analysis (13C-MFA) and experimental measurement results. A model consisting of 451 metabolites and 604 reactions was utilized in flux balance analysis (FBA). The stringency of the flux spaces considering different optimization objectives, i.e. growth rate maximization, ATP maintenance, and NADH/NADPH formation, for flux variance analysis (FVA) was studied in the different modelled conditions. Also a previously uncharacterized exopolysaccharide (EPS) produced by C. acetobutylicum was characterized on monosaccharide level. The major monosaccharide components of the EPS were 40 n-% rhamnose, 34 n-% glucose, 13 n-% mannose, 10 n-% galactose, and 2 n-% arabinose. The EPS was studied to have butanol adsorbing property, 70 (butanol) mg (EPS) g-1 at 37 °C.

AB - The metabolism of butanol producing bacteria Clostridium acetobutylicum was studied in chemostat with glucose limited conditions, butanol stimulus, and as a reference cultivation. COnstraint-Based Reconstruction and Analysis (COBRA) was applied using additional constraints from 13C Metabolic Flux Analysis (13C-MFA) and experimental measurement results. A model consisting of 451 metabolites and 604 reactions was utilized in flux balance analysis (FBA). The stringency of the flux spaces considering different optimization objectives, i.e. growth rate maximization, ATP maintenance, and NADH/NADPH formation, for flux variance analysis (FVA) was studied in the different modelled conditions. Also a previously uncharacterized exopolysaccharide (EPS) produced by C. acetobutylicum was characterized on monosaccharide level. The major monosaccharide components of the EPS were 40 n-% rhamnose, 34 n-% glucose, 13 n-% mannose, 10 n-% galactose, and 2 n-% arabinose. The EPS was studied to have butanol adsorbing property, 70 (butanol) mg (EPS) g-1 at 37 °C.

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KW - butanol stress

KW - C. acetobutylicum

KW - COBRA

KW - exopolysaccharide

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M3 - Article

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