Carbon 13-Metabolic Flux Analysis derived constraint-based metabolic modelling of Clostridium acetobutylicum in stressed chemostat conditions

Janne Wallenius (Corresponding Author), Hannu Maaheimo, Tero Eerikäinen

    Research output: Contribution to journalArticlepeer-review

    12 Citations (Scopus)

    Abstract

    The metabolism of butanol producing bacteria Clostridium acetobutylicum was studied in chemostat with glucose limited conditions, butanol stimulus, and as a reference cultivation. COnstraint-Based Reconstruction and Analysis (COBRA) was applied using additional constraints from 13C Metabolic Flux Analysis (13C-MFA) and experimental measurement results. A model consisting of 451 metabolites and 604 reactions was utilized in flux balance analysis (FBA). The stringency of the flux spaces considering different optimization objectives, i.e. growth rate maximization, ATP maintenance, and NADH/NADPH formation, for flux variance analysis (FVA) was studied in the different modelled conditions. Also a previously uncharacterized exopolysaccharide (EPS) produced by C. acetobutylicum was characterized on monosaccharide level. The major monosaccharide components of the EPS were 40 n-% rhamnose, 34 n-% glucose, 13 n-% mannose, 10 n-% galactose, and 2 n-% arabinose. The EPS was studied to have butanol adsorbing property, 70 (butanol) mg (EPS) g-1 at 37 °C.
    Original languageEnglish
    Pages (from-to)378-386
    JournalBioresource Technology
    Volume219
    DOIs
    Publication statusPublished - 2016
    MoE publication typeA1 Journal article-refereed

    Keywords

    • 13C metabolic flux analysis
    • butanol stress
    • C. acetobutylicum
    • COBRA
    • exopolysaccharide

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