Categorisation of sugar acid dehydratases in Aspergillus niger

Francine A. Motter, Joosu Kuivanen, Hanna Keränen, Satu Hilditch, Merja Penttilä, Peter Richard (Corresponding Author)

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)

    Abstract

    In the genome of Aspergillus niger five genes were identified coding for proteins with homologies to sugar acid dehydratases. The open reading frames were expressed in Saccharomyces cerevisiae and the activities tested with a library of sugar acids. Four genes were identified to code for proteins with activities with sugar acids: an l-galactonate dehydratase (gaaB), two d-galactonate dehydratases (dgdA, dgdB) and an l-rhamnonate dehydratase (lraC). The specificities of the proteins were characterised. The l-galactonate dehydratase had highest activity with l-fuconate, however it is unclear whether the enzyme is involved in l-fuconate catabolism. None of the proteins showed activity with galactaric acid or galactarolactone.

    Original languageEnglish
    Pages (from-to)67-72
    Number of pages6
    JournalFungal Genetics and Biology
    Volume64
    DOIs
    Publication statusPublished - 1 Mar 2014
    MoE publication typeA1 Journal article-refereed

    Keywords

    • 2-Keto-3-deoxy acid
    • D-Galactonate
    • Dehydratase
    • L-Fuconate
    • L-Galactonate
    • L-Rhamnonate

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