Categorisation of sugar acid dehydratases in Aspergillus niger

Francine A. Motter; Joosu Kuivanen; Hanna Keränen; Satu Hilditch; Merja Penttilä; Peter Richard

doi:10.1016/j.fgb.2013.12.006

Categorisation of sugar acid dehydratases in Aspergillus niger

Francine A. Motter
, Joosu Kuivanen
, Hanna Keränen
, Satu Hilditch
, Merja Penttilä
, Peter Richard^*

^*Corresponding author for this work

Federal University of Paraná
VTT (former employee or external)

Research output: Contribution to journal › Article › Scientific › peer-review

15 Citations (Scopus)

Abstract

In the genome of Aspergillus niger five genes were identified coding for proteins with homologies to sugar acid dehydratases. The open reading frames were expressed in Saccharomyces cerevisiae and the activities tested with a library of sugar acids. Four genes were identified to code for proteins with activities with sugar acids: an l-galactonate dehydratase (gaaB), two d-galactonate dehydratases (dgdA, dgdB) and an l-rhamnonate dehydratase (lraC). The specificities of the proteins were characterised. The l-galactonate dehydratase had highest activity with l-fuconate, however it is unclear whether the enzyme is involved in l-fuconate catabolism. None of the proteins showed activity with galactaric acid or galactarolactone.

Original language	English
Pages (from-to)	67-72
Journal	Fungal Genetics and Biology
Volume	64
DOIs	https://doi.org/10.1016/j.fgb.2013.12.006
Publication status	Published - 1 Mar 2014
MoE publication type	A1 Journal article-refereed

Funding

This study was supported by the Academy of Finland through the Sustainable Energy (SusEn) program (Grant 271025) and by the CNPq of Brazil in the form of a cooperation project.

Keywords

2-Keto-3-deoxy acid
D-Galactonate
Dehydratase
L-Fuconate
L-Galactonate
L-Rhamnonate

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10.1016/j.fgb.2013.12.006

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title = "Categorisation of sugar acid dehydratases in Aspergillus niger",

abstract = "In the genome of Aspergillus niger five genes were identified coding for proteins with homologies to sugar acid dehydratases. The open reading frames were expressed in Saccharomyces cerevisiae and the activities tested with a library of sugar acids. Four genes were identified to code for proteins with activities with sugar acids: an l-galactonate dehydratase (gaaB), two d-galactonate dehydratases (dgdA, dgdB) and an l-rhamnonate dehydratase (lraC). The specificities of the proteins were characterised. The l-galactonate dehydratase had highest activity with l-fuconate, however it is unclear whether the enzyme is involved in l-fuconate catabolism. None of the proteins showed activity with galactaric acid or galactarolactone.",

keywords = "2-Keto-3-deoxy acid, D-Galactonate, Dehydratase, L-Fuconate, L-Galactonate, L-Rhamnonate",

author = "Motter, \{Francine A.\} and Joosu Kuivanen and Hanna Ker{\"a}nen and Satu Hilditch and Merja Penttil{\"a} and Peter Richard",

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T1 - Categorisation of sugar acid dehydratases in Aspergillus niger

AU - Motter, Francine A.

AU - Kuivanen, Joosu

AU - Keränen, Hanna

AU - Hilditch, Satu

AU - Penttilä, Merja

AU - Richard, Peter

N1 - CA2: BA3112 CA2: BA311 ISI: GENETICS & HEREDITY

PY - 2014/3/1

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N2 - In the genome of Aspergillus niger five genes were identified coding for proteins with homologies to sugar acid dehydratases. The open reading frames were expressed in Saccharomyces cerevisiae and the activities tested with a library of sugar acids. Four genes were identified to code for proteins with activities with sugar acids: an l-galactonate dehydratase (gaaB), two d-galactonate dehydratases (dgdA, dgdB) and an l-rhamnonate dehydratase (lraC). The specificities of the proteins were characterised. The l-galactonate dehydratase had highest activity with l-fuconate, however it is unclear whether the enzyme is involved in l-fuconate catabolism. None of the proteins showed activity with galactaric acid or galactarolactone.

AB - In the genome of Aspergillus niger five genes were identified coding for proteins with homologies to sugar acid dehydratases. The open reading frames were expressed in Saccharomyces cerevisiae and the activities tested with a library of sugar acids. Four genes were identified to code for proteins with activities with sugar acids: an l-galactonate dehydratase (gaaB), two d-galactonate dehydratases (dgdA, dgdB) and an l-rhamnonate dehydratase (lraC). The specificities of the proteins were characterised. The l-galactonate dehydratase had highest activity with l-fuconate, however it is unclear whether the enzyme is involved in l-fuconate catabolism. None of the proteins showed activity with galactaric acid or galactarolactone.

KW - 2-Keto-3-deoxy acid

KW - D-Galactonate

KW - Dehydratase

KW - L-Fuconate

KW - L-Galactonate

KW - L-Rhamnonate

UR - https://www.scopus.com/pages/publications/84893753642

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DO - 10.1016/j.fgb.2013.12.006

M3 - Article

C2 - 24382357

AN - SCOPUS:84893753642

SN - 1087-1845

VL - 64

SP - 67

EP - 72

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

ER -

Categorisation of sugar acid dehydratases in Aspergillus niger

Abstract

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Keywords

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