Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 11-16 |
| Journal | Current Genetics |
| Volume | 43 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2003 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- pmt gene
- Protein glycosylation
- 0-Mannosylation
- Secretion
Cite this
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cDNA encoding protein 0-mannosyltransferase from the filamentous fungus Trichoderma reesei; functional equivalence to Saccharomyces cerevisiae PMT2. / Zakrzewska, Anna; Migdalski, Andrzej; Saloheimo, Markku; Penttilä, Merja; Palamarczyk, Grazyna; Kruszewska, Joanna (Corresponding Author).
In: Current Genetics, Vol. 43, No. 1, 2003, p. 11-16.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - cDNA encoding protein 0-mannosyltransferase from the filamentous fungus Trichoderma reesei; functional equivalence to Saccharomyces cerevisiae PMT2
AU - Zakrzewska, Anna
AU - Migdalski, Andrzej
AU - Saloheimo, Markku
AU - Penttilä, Merja
AU - Palamarczyk, Grazyna
AU - Kruszewska, Joanna
PY - 2003
Y1 - 2003
N2 - O-Mannosylation is suggested to be essential for protein secretion in Trichoderma reesei. In protein O-glycosylation, the first mannosyl residue is transferred to a serine or threonine hydroxyl group of the protein from dolichyl phosphate mannose by protein O-mannosyltransferase. In Saccharomyces cerevisiae, seven PMT genes have been cloned coding for these enzymes. In the present work, the characterisation of the pmt1 cDNA from T. reesei is reported. Sequence analysis of the predicted protein revealed the highest similarity to Schizosaccharomyces pombe Pmt and to Pmt4p of Saccharomyces cerevisiae. In contrast, expression of the T. reesei cDNA in various S. cerevisiae pmt mutants showed functional similarity to the yeast Pmt2 protein.
AB - O-Mannosylation is suggested to be essential for protein secretion in Trichoderma reesei. In protein O-glycosylation, the first mannosyl residue is transferred to a serine or threonine hydroxyl group of the protein from dolichyl phosphate mannose by protein O-mannosyltransferase. In Saccharomyces cerevisiae, seven PMT genes have been cloned coding for these enzymes. In the present work, the characterisation of the pmt1 cDNA from T. reesei is reported. Sequence analysis of the predicted protein revealed the highest similarity to Schizosaccharomyces pombe Pmt and to Pmt4p of Saccharomyces cerevisiae. In contrast, expression of the T. reesei cDNA in various S. cerevisiae pmt mutants showed functional similarity to the yeast Pmt2 protein.
KW - pmt gene
KW - Protein glycosylation
KW - 0-Mannosylation
KW - Secretion
U2 - 10.1007/s00294-003-0368-5
DO - 10.1007/s00294-003-0368-5
M3 - Article
VL - 43
SP - 11
EP - 16
JO - Current Genetics
JF - Current Genetics
SN - 0172-8083
IS - 1
ER -