Cellular interactions of surface modified nanoporous silicon particles

Luis M. Bimbo; Mirkka Sarparanta; Ermei Mäkilä; Timo Laaksonen; Päivi Laaksonen; Jarno Salonen; Markus B. Linder; Jouni Hirvonen; Anu J. Airaksinen; Hélder A. Santos

doi:10.1039/c2nr30397c

Cellular interactions of surface modified nanoporous silicon particles

Luis M. Bimbo (Corresponding Author), Mirkka Sarparanta, Ermei Mäkilä, Timo Laaksonen, Päivi Laaksonen, Jarno Salonen, Markus B. Linder, Jouni Hirvonen, Anu J. Airaksinen, Hélder A. Santos (Corresponding Author)

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

51 Citations (Scopus)

Abstract

In this study, the self-assembly of hydrophobin class II (HFBII) on the surface of thermally hydrocarbonized porous silicon (THCPSi) nanoparticles was investigated. The HFBII-coating converted the hydrophobic particles into more hydrophilic ones, improved the particles' cell viability in both HT-29 and Caco-2 cell lines compared to uncoated particles, and enhanced the particles' cellular association. The amount of HFBII adsorbed onto the particles was also successfully quantified by both the BCA assay and a HPLC method. Importantly, the permeation of a poorly water-soluble drug, indomethacin, loaded into THCPSi particles across Caco-2 monolayers was not affected by the protein coating. In addition, ¹²⁵I-radiolabelled HFBII did not extensively permeate the Caco-2 monolayer and was found to be stably adsorbed onto the THCPSi nanoparticles incubated in pH 7.4, which renders the particles the possibility for further track-imaging applications. The results highlight the potential of HFBII coating for improving wettability, increasing biocompatibility and possible intestinal association of PSi nanoparticulates for drug delivery applications.

Original language	English
Pages (from-to)	3184-3192
Journal	Nanoscale
Volume	4
Issue number	10
DOIs	https://doi.org/10.1039/c2nr30397c
Publication status	Published - 2012
MoE publication type	A1 Journal article-refereed

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title = "Cellular interactions of surface modified nanoporous silicon particles",

abstract = "In this study, the self-assembly of hydrophobin class II (HFBII) on the surface of thermally hydrocarbonized porous silicon (THCPSi) nanoparticles was investigated. The HFBII-coating converted the hydrophobic particles into more hydrophilic ones, improved the particles' cell viability in both HT-29 and Caco-2 cell lines compared to uncoated particles, and enhanced the particles' cellular association. The amount of HFBII adsorbed onto the particles was also successfully quantified by both the BCA assay and a HPLC method. Importantly, the permeation of a poorly water-soluble drug, indomethacin, loaded into THCPSi particles across Caco-2 monolayers was not affected by the protein coating. In addition, 125I-radiolabelled HFBII did not extensively permeate the Caco-2 monolayer and was found to be stably adsorbed onto the THCPSi nanoparticles incubated in pH 7.4, which renders the particles the possibility for further track-imaging applications. The results highlight the potential of HFBII coating for improving wettability, increasing biocompatibility and possible intestinal association of PSi nanoparticulates for drug delivery applications.",

author = "Bimbo, {Luis M.} and Mirkka Sarparanta and Ermei M{\"a}kil{\"a} and Timo Laaksonen and P{\"a}ivi Laaksonen and Jarno Salonen and Linder, {Markus B.} and Jouni Hirvonen and Airaksinen, {Anu J.} and Santos, {H{\'e}lder A.}",

year = "2012",

doi = "10.1039/c2nr30397c",

language = "English",

volume = "4",

pages = "3184--3192",

journal = "Nanoscale",

issn = "2040-3364",

publisher = "Royal Society of Chemistry RSC",

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TY - JOUR

T1 - Cellular interactions of surface modified nanoporous silicon particles

AU - Bimbo, Luis M.

AU - Sarparanta, Mirkka

AU - Mäkilä, Ermei

AU - Laaksonen, Timo

AU - Laaksonen, Päivi

AU - Salonen, Jarno

AU - Linder, Markus B.

AU - Hirvonen, Jouni

AU - Airaksinen, Anu J.

AU - Santos, Hélder A.

PY - 2012

Y1 - 2012

N2 - In this study, the self-assembly of hydrophobin class II (HFBII) on the surface of thermally hydrocarbonized porous silicon (THCPSi) nanoparticles was investigated. The HFBII-coating converted the hydrophobic particles into more hydrophilic ones, improved the particles' cell viability in both HT-29 and Caco-2 cell lines compared to uncoated particles, and enhanced the particles' cellular association. The amount of HFBII adsorbed onto the particles was also successfully quantified by both the BCA assay and a HPLC method. Importantly, the permeation of a poorly water-soluble drug, indomethacin, loaded into THCPSi particles across Caco-2 monolayers was not affected by the protein coating. In addition, 125I-radiolabelled HFBII did not extensively permeate the Caco-2 monolayer and was found to be stably adsorbed onto the THCPSi nanoparticles incubated in pH 7.4, which renders the particles the possibility for further track-imaging applications. The results highlight the potential of HFBII coating for improving wettability, increasing biocompatibility and possible intestinal association of PSi nanoparticulates for drug delivery applications.

AB - In this study, the self-assembly of hydrophobin class II (HFBII) on the surface of thermally hydrocarbonized porous silicon (THCPSi) nanoparticles was investigated. The HFBII-coating converted the hydrophobic particles into more hydrophilic ones, improved the particles' cell viability in both HT-29 and Caco-2 cell lines compared to uncoated particles, and enhanced the particles' cellular association. The amount of HFBII adsorbed onto the particles was also successfully quantified by both the BCA assay and a HPLC method. Importantly, the permeation of a poorly water-soluble drug, indomethacin, loaded into THCPSi particles across Caco-2 monolayers was not affected by the protein coating. In addition, 125I-radiolabelled HFBII did not extensively permeate the Caco-2 monolayer and was found to be stably adsorbed onto the THCPSi nanoparticles incubated in pH 7.4, which renders the particles the possibility for further track-imaging applications. The results highlight the potential of HFBII coating for improving wettability, increasing biocompatibility and possible intestinal association of PSi nanoparticulates for drug delivery applications.

U2 - 10.1039/c2nr30397c

DO - 10.1039/c2nr30397c

M3 - Article

SN - 2040-3364

VL - 4

SP - 3184

EP - 3192

JO - Nanoscale

JF - Nanoscale

IS - 10

ER -

Cellular interactions of surface modified nanoporous silicon particles

Abstract

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