Cellular interactions of surface modified nanoporous silicon particles

Luis M. Bimbo (Corresponding Author), Mirkka Sarparanta, Ermei Mäkilä, Timo Laaksonen, Päivi Laaksonen, Jarno Salonen, Markus B. Linder, Jouni Hirvonen, Anu J. Airaksinen, Hélder A. Santos (Corresponding Author)

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Abstract

In this study, the self-assembly of hydrophobin class II (HFBII) on the surface of thermally hydrocarbonized porous silicon (THCPSi) nanoparticles was investigated. The HFBII-coating converted the hydrophobic particles into more hydrophilic ones, improved the particles' cell viability in both HT-29 and Caco-2 cell lines compared to uncoated particles, and enhanced the particles' cellular association. The amount of HFBII adsorbed onto the particles was also successfully quantified by both the BCA assay and a HPLC method. Importantly, the permeation of a poorly water-soluble drug, indomethacin, loaded into THCPSi particles across Caco-2 monolayers was not affected by the protein coating. In addition, 125I-radiolabelled HFBII did not extensively permeate the Caco-2 monolayer and was found to be stably adsorbed onto the THCPSi nanoparticles incubated in pH 7.4, which renders the particles the possibility for further track-imaging applications. The results highlight the potential of HFBII coating for improving wettability, increasing biocompatibility and possible intestinal association of PSi nanoparticulates for drug delivery applications.
Original languageEnglish
Pages (from-to)3184-3192
JournalNanoscale
Volume4
Issue number10
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

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Bimbo, L. M., Sarparanta, M., Mäkilä, E., Laaksonen, T., Laaksonen, P., Salonen, J., Linder, M. B., Hirvonen, J., Airaksinen, A. J., & Santos, H. A. (2012). Cellular interactions of surface modified nanoporous silicon particles. Nanoscale, 4(10), 3184-3192. https://doi.org/10.1039/c2nr30397c