Cellular responses to protein production in the filamentous fungus Trichoderma reesei

Tiina Pakula, Mikko Arvas, Jari Rautio, Bart Smit, Marilyn Wiebe, Heini Koivistoinen, Markku Saloheimo, Merja Penttilä

Research output: Contribution to conferenceConference AbstractScientific

Abstract

The filamentous fungus Trichoderma reesei is known as an efficient producer of a variety of cellulases and hemicellulases, and it is used as an industrial host organism for production of the fungal enzymes as well as for heterologous proteins. Production of secreted proteins in large quantities, or production of heterologous proteins originating from distantly related organisms, are known to provoke stress responses in the cells. The known stress responses include e.g. activation of the unfolded protein response (UPR) pathway resulting in induction of a number of genes needed in enhancement of protein folding and transport. The fungal cells have also a feed-back mechanism to reduce the load in the secretory pathway by negative transcriptional regulation of genes encoding the major secreted proteins.

The availability of the genome sequence information has made it possible to apply genome-wide approaches in studies of the cellular responses to protein production under different conditions and to obtain further information on protein production and factors influencing it at different physiological conditions. We have applied transcriptome and proteome analysis to study the effects of production of heterologous proteins in T. reesei, as well as to analyse different protein production conditions in batch bioreactor and chemostat cultures. Specifically, we have analysed protein production and cellular responses in carbon-limited chemostat cultures at different specific growth rates and cell densities.
Original languageEnglish
Publication statusPublished - 2008
Event9th European Conference on Fungal Genetics - Edinburgh, United Kingdom
Duration: 5 Aug 20088 Aug 2008

Conference

Conference9th European Conference on Fungal Genetics
CountryUnited Kingdom
CityEdinburgh
Period5/08/088/08/08

Fingerprint

Trichoderma reesei
fungi
proteins
stress response
unfolded protein response
protein folding
genome
cellulases
protein transport
organisms
cells
major genes
bioreactors
proteome
transcriptome
specific growth rate
carbon
enzymes

Cite this

Pakula, T., Arvas, M., Rautio, J., Smit, B., Wiebe, M., Koivistoinen, H., ... Penttilä, M. (2008). Cellular responses to protein production in the filamentous fungus Trichoderma reesei. Abstract from 9th European Conference on Fungal Genetics, Edinburgh, United Kingdom.
Pakula, Tiina ; Arvas, Mikko ; Rautio, Jari ; Smit, Bart ; Wiebe, Marilyn ; Koivistoinen, Heini ; Saloheimo, Markku ; Penttilä, Merja. / Cellular responses to protein production in the filamentous fungus Trichoderma reesei. Abstract from 9th European Conference on Fungal Genetics, Edinburgh, United Kingdom.
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abstract = "The filamentous fungus Trichoderma reesei is known as an efficient producer of a variety of cellulases and hemicellulases, and it is used as an industrial host organism for production of the fungal enzymes as well as for heterologous proteins. Production of secreted proteins in large quantities, or production of heterologous proteins originating from distantly related organisms, are known to provoke stress responses in the cells. The known stress responses include e.g. activation of the unfolded protein response (UPR) pathway resulting in induction of a number of genes needed in enhancement of protein folding and transport. The fungal cells have also a feed-back mechanism to reduce the load in the secretory pathway by negative transcriptional regulation of genes encoding the major secreted proteins.The availability of the genome sequence information has made it possible to apply genome-wide approaches in studies of the cellular responses to protein production under different conditions and to obtain further information on protein production and factors influencing it at different physiological conditions. We have applied transcriptome and proteome analysis to study the effects of production of heterologous proteins in T. reesei, as well as to analyse different protein production conditions in batch bioreactor and chemostat cultures. Specifically, we have analysed protein production and cellular responses in carbon-limited chemostat cultures at different specific growth rates and cell densities.",
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Pakula, T, Arvas, M, Rautio, J, Smit, B, Wiebe, M, Koivistoinen, H, Saloheimo, M & Penttilä, M 2008, 'Cellular responses to protein production in the filamentous fungus Trichoderma reesei' 9th European Conference on Fungal Genetics, Edinburgh, United Kingdom, 5/08/08 - 8/08/08, .

Cellular responses to protein production in the filamentous fungus Trichoderma reesei. / Pakula, Tiina; Arvas, Mikko; Rautio, Jari; Smit, Bart; Wiebe, Marilyn; Koivistoinen, Heini; Saloheimo, Markku; Penttilä, Merja.

2008. Abstract from 9th European Conference on Fungal Genetics, Edinburgh, United Kingdom.

Research output: Contribution to conferenceConference AbstractScientific

TY - CONF

T1 - Cellular responses to protein production in the filamentous fungus Trichoderma reesei

AU - Pakula, Tiina

AU - Arvas, Mikko

AU - Rautio, Jari

AU - Smit, Bart

AU - Wiebe, Marilyn

AU - Koivistoinen, Heini

AU - Saloheimo, Markku

AU - Penttilä, Merja

N1 - CA2: TK402

PY - 2008

Y1 - 2008

N2 - The filamentous fungus Trichoderma reesei is known as an efficient producer of a variety of cellulases and hemicellulases, and it is used as an industrial host organism for production of the fungal enzymes as well as for heterologous proteins. Production of secreted proteins in large quantities, or production of heterologous proteins originating from distantly related organisms, are known to provoke stress responses in the cells. The known stress responses include e.g. activation of the unfolded protein response (UPR) pathway resulting in induction of a number of genes needed in enhancement of protein folding and transport. The fungal cells have also a feed-back mechanism to reduce the load in the secretory pathway by negative transcriptional regulation of genes encoding the major secreted proteins.The availability of the genome sequence information has made it possible to apply genome-wide approaches in studies of the cellular responses to protein production under different conditions and to obtain further information on protein production and factors influencing it at different physiological conditions. We have applied transcriptome and proteome analysis to study the effects of production of heterologous proteins in T. reesei, as well as to analyse different protein production conditions in batch bioreactor and chemostat cultures. Specifically, we have analysed protein production and cellular responses in carbon-limited chemostat cultures at different specific growth rates and cell densities.

AB - The filamentous fungus Trichoderma reesei is known as an efficient producer of a variety of cellulases and hemicellulases, and it is used as an industrial host organism for production of the fungal enzymes as well as for heterologous proteins. Production of secreted proteins in large quantities, or production of heterologous proteins originating from distantly related organisms, are known to provoke stress responses in the cells. The known stress responses include e.g. activation of the unfolded protein response (UPR) pathway resulting in induction of a number of genes needed in enhancement of protein folding and transport. The fungal cells have also a feed-back mechanism to reduce the load in the secretory pathway by negative transcriptional regulation of genes encoding the major secreted proteins.The availability of the genome sequence information has made it possible to apply genome-wide approaches in studies of the cellular responses to protein production under different conditions and to obtain further information on protein production and factors influencing it at different physiological conditions. We have applied transcriptome and proteome analysis to study the effects of production of heterologous proteins in T. reesei, as well as to analyse different protein production conditions in batch bioreactor and chemostat cultures. Specifically, we have analysed protein production and cellular responses in carbon-limited chemostat cultures at different specific growth rates and cell densities.

UR - http://www.fgsc.net/ECFG9/ecfg_9_parallel_session_7.htm

M3 - Conference Abstract

ER -

Pakula T, Arvas M, Rautio J, Smit B, Wiebe M, Koivistoinen H et al. Cellular responses to protein production in the filamentous fungus Trichoderma reesei. 2008. Abstract from 9th European Conference on Fungal Genetics, Edinburgh, United Kingdom.