TY - CHAP
T1 - Cellular responses to protein production in the filamentous fungus Trichoderma reesei
AU - Pakula, Tiina
PY - 2007
Y1 - 2007
N2 - The filamentous fungus Trichoderma reesei is known as an
efficient producer of a variety of extracellular enzymes,
the major products being cellulases and hemicellulases
e.g. cellobiohydrolases, endoglucanases,
Beta-glucosidases,
xylanases, and hemicellulose side-chain cleaving enzymes.
Altogether, analysis of the genome sequence has revealed
over 200 genes classified in glycoside hydrolase gene
families. T. reesei has potential to produce
extracellular proteins in very large quantities, and it
has been used as an industrial host organism for
production of both the fungal enzymes as well as for
heterologous proteins.
Production of secreted proteins in large quantities or
production of the heterologous proteins originating from
distantly related organisms challenge the capability of
the cells to fold and transport the proteins, and are
known to provoke stress responses in the cell. Impaired
protein folding in the endoplasmic reticulum (ER)
activates the unfolded protein response pathway (UPR)
which result in induction of a number of genes involved
e.g. in folding, glycosylation and transport. The fungal
cells have also a feed-back mechanism to reduce the load
in the secretory pathway by negative transcriptional
regulation of genes encoding the major secreted proteins.
The availability of the genome sequence information has
made it possible to apply genome-wide approaches in
studies of the cellular responses to protein production
under different conditions.
Specifically, we have compared the effects of production
of two different heterologous proteins, human tPA and
Melanocarpus albomyces laccase, in T. reesei using
proteome and transcriptome data. The analysis showed a
clear difference between the responses induced by the
proteins, the main difference being in the induction of
the UPR pathway. Furthermore, in order to obtain
information on protein production at different
physiological conditions we have carried out
transcriptome and proteome analysis of carbon-limited
chemostat cultures of T. reesei under different
conditions, e.g. at different specific growth rates and
cell density.
AB - The filamentous fungus Trichoderma reesei is known as an
efficient producer of a variety of extracellular enzymes,
the major products being cellulases and hemicellulases
e.g. cellobiohydrolases, endoglucanases,
Beta-glucosidases,
xylanases, and hemicellulose side-chain cleaving enzymes.
Altogether, analysis of the genome sequence has revealed
over 200 genes classified in glycoside hydrolase gene
families. T. reesei has potential to produce
extracellular proteins in very large quantities, and it
has been used as an industrial host organism for
production of both the fungal enzymes as well as for
heterologous proteins.
Production of secreted proteins in large quantities or
production of the heterologous proteins originating from
distantly related organisms challenge the capability of
the cells to fold and transport the proteins, and are
known to provoke stress responses in the cell. Impaired
protein folding in the endoplasmic reticulum (ER)
activates the unfolded protein response pathway (UPR)
which result in induction of a number of genes involved
e.g. in folding, glycosylation and transport. The fungal
cells have also a feed-back mechanism to reduce the load
in the secretory pathway by negative transcriptional
regulation of genes encoding the major secreted proteins.
The availability of the genome sequence information has
made it possible to apply genome-wide approaches in
studies of the cellular responses to protein production
under different conditions.
Specifically, we have compared the effects of production
of two different heterologous proteins, human tPA and
Melanocarpus albomyces laccase, in T. reesei using
proteome and transcriptome data. The analysis showed a
clear difference between the responses induced by the
proteins, the main difference being in the induction of
the UPR pathway. Furthermore, in order to obtain
information on protein production at different
physiological conditions we have carried out
transcriptome and proteome analysis of carbon-limited
chemostat cultures of T. reesei under different
conditions, e.g. at different specific growth rates and
cell density.
M3 - Conference abstract in proceedings
SN - 978-951-38-6313-5
T3 - VTT Symposium
SP - 40
BT - 3rd European Federation of Biotechnology Conference
PB - VTT Technical Research Centre of Finland
CY - Espoo
T2 - 3rd European Federation of Biotechnology Conference
Y2 - 13 June 2007 through 16 June 2007
ER -