Cellular responses to secretion stress in Trichoderma reesei

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Trichoderma reesei is known for its extremely high capacity of protein secretion. High loads of protein, and especially foreign protein, in the secretory pathway form a challenge to the production organism and expose it to secretion stress. Unfolded protein response (UPR) denotes the induction mechanism of genes encoding ER-resident chaperones and foldases and numerous other genes involved in protein secretion. This induction is triggered when unfolded proteins accumulate into the ER. We have cloned the transcription factor involved in UPR induction, HACI, from T. reesei. Our results indicate that the hac1 gene is activated by a dual mechanism operational at the mRNA level. This mechanism includes a splicing event of an unconventional intron of only 20 nt in length and a truncation of the mRNA at the 5' flanking region. This truncation removes an upstream open reading frame from the mRNA, and we have shown that these uORFs are involved in translational control of the HAC1 protein formation. We have observed that concurrently with the induction of the UPR pathway, the genes encoding secreted proteins are rapidly down-regulated in Trichoderma reesei. This type of regulation can be caused by different secretion inhibitors and by foreign protein expression. The down-regulation is dependent on the promoter of the affected gene, suggesting that it is functional at the transcriptional level. The down-regulation of genes encoding secreted proteins during secretion stress has not been reported before from any other experimental system and thus it could be unique for filamentous fungi.
Original languageEnglish
Title of host publication22nd Fungal Genetics Conference at Asilomar
Subtitle of host publicationAbstracts from the 2003 Fungal Genetics Conference at Asilomar
DOIs
Publication statusPublished - 2003
Event22nd Fungal Genetics Conference - Asilomar, United States
Duration: 18 Mar 200322 Mar 2003

Publication series

SeriesFungal Genetics Reports
NumberArticle 18
Volume50

Conference

Conference22nd Fungal Genetics Conference
CountryUnited States
CityAsilomar
Period18/03/0322/03/03

Fingerprint

Trichoderma reesei
secretion
unfolded protein response
protein secretion
genes
proteins
open reading frames
introns
transcription factors
protein synthesis
promoter regions
fungi
organisms

Cite this

Saloheimo, M., Pakula, T., Arvas, M., Valkonen, M., & Penttilä, M. (2003). Cellular responses to secretion stress in Trichoderma reesei. In 22nd Fungal Genetics Conference at Asilomar: Abstracts from the 2003 Fungal Genetics Conference at Asilomar [422] Fungal Genetics Reports, No. Article 18, Vol.. 50 https://doi.org/10.4148/1941-4765.1164
Saloheimo, Markku ; Pakula, Tiina ; Arvas, Mikko ; Valkonen, Mari ; Penttilä, Merja. / Cellular responses to secretion stress in Trichoderma reesei. 22nd Fungal Genetics Conference at Asilomar: Abstracts from the 2003 Fungal Genetics Conference at Asilomar. 2003. (Fungal Genetics Reports; No. Article 18, Vol. 50).
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Saloheimo, M, Pakula, T, Arvas, M, Valkonen, M & Penttilä, M 2003, Cellular responses to secretion stress in Trichoderma reesei. in 22nd Fungal Genetics Conference at Asilomar: Abstracts from the 2003 Fungal Genetics Conference at Asilomar., 422, Fungal Genetics Reports, no. Article 18, vol. 50, 22nd Fungal Genetics Conference, Asilomar, United States, 18/03/03. https://doi.org/10.4148/1941-4765.1164

Cellular responses to secretion stress in Trichoderma reesei. / Saloheimo, Markku; Pakula, Tiina; Arvas, Mikko; Valkonen, Mari; Penttilä, Merja.

22nd Fungal Genetics Conference at Asilomar: Abstracts from the 2003 Fungal Genetics Conference at Asilomar. 2003. 422 (Fungal Genetics Reports; No. Article 18, Vol. 50).

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Cellular responses to secretion stress in Trichoderma reesei

AU - Saloheimo, Markku

AU - Pakula, Tiina

AU - Arvas, Mikko

AU - Valkonen, Mari

AU - Penttilä, Merja

PY - 2003

Y1 - 2003

N2 - Trichoderma reesei is known for its extremely high capacity of protein secretion. High loads of protein, and especially foreign protein, in the secretory pathway form a challenge to the production organism and expose it to secretion stress. Unfolded protein response (UPR) denotes the induction mechanism of genes encoding ER-resident chaperones and foldases and numerous other genes involved in protein secretion. This induction is triggered when unfolded proteins accumulate into the ER. We have cloned the transcription factor involved in UPR induction, HACI, from T. reesei. Our results indicate that the hac1 gene is activated by a dual mechanism operational at the mRNA level. This mechanism includes a splicing event of an unconventional intron of only 20 nt in length and a truncation of the mRNA at the 5' flanking region. This truncation removes an upstream open reading frame from the mRNA, and we have shown that these uORFs are involved in translational control of the HAC1 protein formation. We have observed that concurrently with the induction of the UPR pathway, the genes encoding secreted proteins are rapidly down-regulated in Trichoderma reesei. This type of regulation can be caused by different secretion inhibitors and by foreign protein expression. The down-regulation is dependent on the promoter of the affected gene, suggesting that it is functional at the transcriptional level. The down-regulation of genes encoding secreted proteins during secretion stress has not been reported before from any other experimental system and thus it could be unique for filamentous fungi.

AB - Trichoderma reesei is known for its extremely high capacity of protein secretion. High loads of protein, and especially foreign protein, in the secretory pathway form a challenge to the production organism and expose it to secretion stress. Unfolded protein response (UPR) denotes the induction mechanism of genes encoding ER-resident chaperones and foldases and numerous other genes involved in protein secretion. This induction is triggered when unfolded proteins accumulate into the ER. We have cloned the transcription factor involved in UPR induction, HACI, from T. reesei. Our results indicate that the hac1 gene is activated by a dual mechanism operational at the mRNA level. This mechanism includes a splicing event of an unconventional intron of only 20 nt in length and a truncation of the mRNA at the 5' flanking region. This truncation removes an upstream open reading frame from the mRNA, and we have shown that these uORFs are involved in translational control of the HAC1 protein formation. We have observed that concurrently with the induction of the UPR pathway, the genes encoding secreted proteins are rapidly down-regulated in Trichoderma reesei. This type of regulation can be caused by different secretion inhibitors and by foreign protein expression. The down-regulation is dependent on the promoter of the affected gene, suggesting that it is functional at the transcriptional level. The down-regulation of genes encoding secreted proteins during secretion stress has not been reported before from any other experimental system and thus it could be unique for filamentous fungi.

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U2 - 10.4148/1941-4765.1164

DO - 10.4148/1941-4765.1164

M3 - Conference abstract in proceedings

T3 - Fungal Genetics Reports

BT - 22nd Fungal Genetics Conference at Asilomar

ER -

Saloheimo M, Pakula T, Arvas M, Valkonen M, Penttilä M. Cellular responses to secretion stress in Trichoderma reesei. In 22nd Fungal Genetics Conference at Asilomar: Abstracts from the 2003 Fungal Genetics Conference at Asilomar. 2003. 422. (Fungal Genetics Reports; No. Article 18, Vol. 50). https://doi.org/10.4148/1941-4765.1164