Cellulose as a novel substrate for lateral flow assay

Research output: Contribution to journalArticleScientificpeer-review

6 Citations (Scopus)

Abstract

Bioactive paper is a product that includes functionalities based on selective reactions of biomolecules such as enzymes or antibodies. Lateral flow assay (LFA) tests are devices aimed at detecting the presence of a specific analyte in a sample liquid. The test sample flows along a membrane via capillary action. This article describes how a fibrous network should be modified in order to use paper as a membrane material in a lateral flow immunoassay (LFIA) test. Mass production methods of paper and its suitability for the rapid roll-to-roll manufacture of test strips create the possibility to significantly decrease the manufacturing costs of LFA membranes and to find new potential applications for LFA tests within paper and board products in which a LFA test could be integrated. Use of 80 g/m2 paper made of unbeaten, bleached eucalyptus pulp with a dosage of 2.5 g/kg PAE, as a membrane material for a non-competitive immunoassay test for hemoglobin was demonstrated. Furthermore, advantages of this paper in making microfluidic channels on paper and an idea to simplify manufacturing of LFA tests by utilising good mechanical properties of the paper membrane were also presented.
Original languageEnglish
Pages (from-to)536-550
Number of pages15
JournalNordic Pulp and Paper Research Journal
Volume25
Issue number4
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

Fingerprint

Cellulose
cellulose
Assays
assay
Membranes
substrate
assays
Substrates
membrane
testing
manufacturing
immunoassay
Bleached pulp
immunoassays
Hemoglobin
Biomolecules
Microfluidics
Antibodies
Hemoglobins
papermaking

Keywords

  • Bioactive paper
  • lateral flow immunoassay test
  • immunochromatographic test
  • hemoglobin
  • capillary flow

Cite this

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title = "Cellulose as a novel substrate for lateral flow assay",
abstract = "Bioactive paper is a product that includes functionalities based on selective reactions of biomolecules such as enzymes or antibodies. Lateral flow assay (LFA) tests are devices aimed at detecting the presence of a specific analyte in a sample liquid. The test sample flows along a membrane via capillary action. This article describes how a fibrous network should be modified in order to use paper as a membrane material in a lateral flow immunoassay (LFIA) test. Mass production methods of paper and its suitability for the rapid roll-to-roll manufacture of test strips create the possibility to significantly decrease the manufacturing costs of LFA membranes and to find new potential applications for LFA tests within paper and board products in which a LFA test could be integrated. Use of 80 g/m2 paper made of unbeaten, bleached eucalyptus pulp with a dosage of 2.5 g/kg PAE, as a membrane material for a non-competitive immunoassay test for hemoglobin was demonstrated. Furthermore, advantages of this paper in making microfluidic channels on paper and an idea to simplify manufacturing of LFA tests by utilising good mechanical properties of the paper membrane were also presented.",
keywords = "Bioactive paper, lateral flow immunoassay test, immunochromatographic test, hemoglobin, capillary flow",
author = "Timo Lappalainen and Tuija Teerinen and Pia Vento and Leena Hakalahti and Tomi Erho",
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Cellulose as a novel substrate for lateral flow assay. / Lappalainen, Timo; Teerinen, Tuija; Vento, Pia; Hakalahti, Leena; Erho, Tomi.

In: Nordic Pulp and Paper Research Journal, Vol. 25, No. 4, 2010, p. 536-550.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Cellulose as a novel substrate for lateral flow assay

AU - Lappalainen, Timo

AU - Teerinen, Tuija

AU - Vento, Pia

AU - Hakalahti, Leena

AU - Erho, Tomi

N1 - Project code: 31738

PY - 2010

Y1 - 2010

N2 - Bioactive paper is a product that includes functionalities based on selective reactions of biomolecules such as enzymes or antibodies. Lateral flow assay (LFA) tests are devices aimed at detecting the presence of a specific analyte in a sample liquid. The test sample flows along a membrane via capillary action. This article describes how a fibrous network should be modified in order to use paper as a membrane material in a lateral flow immunoassay (LFIA) test. Mass production methods of paper and its suitability for the rapid roll-to-roll manufacture of test strips create the possibility to significantly decrease the manufacturing costs of LFA membranes and to find new potential applications for LFA tests within paper and board products in which a LFA test could be integrated. Use of 80 g/m2 paper made of unbeaten, bleached eucalyptus pulp with a dosage of 2.5 g/kg PAE, as a membrane material for a non-competitive immunoassay test for hemoglobin was demonstrated. Furthermore, advantages of this paper in making microfluidic channels on paper and an idea to simplify manufacturing of LFA tests by utilising good mechanical properties of the paper membrane were also presented.

AB - Bioactive paper is a product that includes functionalities based on selective reactions of biomolecules such as enzymes or antibodies. Lateral flow assay (LFA) tests are devices aimed at detecting the presence of a specific analyte in a sample liquid. The test sample flows along a membrane via capillary action. This article describes how a fibrous network should be modified in order to use paper as a membrane material in a lateral flow immunoassay (LFIA) test. Mass production methods of paper and its suitability for the rapid roll-to-roll manufacture of test strips create the possibility to significantly decrease the manufacturing costs of LFA membranes and to find new potential applications for LFA tests within paper and board products in which a LFA test could be integrated. Use of 80 g/m2 paper made of unbeaten, bleached eucalyptus pulp with a dosage of 2.5 g/kg PAE, as a membrane material for a non-competitive immunoassay test for hemoglobin was demonstrated. Furthermore, advantages of this paper in making microfluidic channels on paper and an idea to simplify manufacturing of LFA tests by utilising good mechanical properties of the paper membrane were also presented.

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KW - immunochromatographic test

KW - hemoglobin

KW - capillary flow

U2 - 10.3183/NPPRJ-2010-25-04-p536-550

DO - 10.3183/NPPRJ-2010-25-04-p536-550

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