TY - CHAP
T1 - Cereal Grain Structure by Microscopic Analysis
AU - Holopainen, Ulla
AU - Raulio, Mari
PY - 2016
Y1 - 2016
N2 - Microscopy techniques can be utilized in studying the structure of cereal grains, their processing or end products. By stereomicroscope, the appearance of cereal samples can be recorded. For light microscopy, samples are usually fixed, embedded and cut into sections for staining with different dyes in order to visualize various components present in cereals. Less sample preparation is needed for confocal laser scanning microscopy (CLSM), which enables optical sectioning of the sample. Scanning electron microscopy (SEM) gives information on the structure with higher magnification, when high resolution is required. Depending on which structural level is of interest, these techniques can be used in revealing the structural differences between processes, enzymatic treatments, etc. Several examples are presented on the visualization of grain structures and components and their changes due to processing.
AB - Microscopy techniques can be utilized in studying the structure of cereal grains, their processing or end products. By stereomicroscope, the appearance of cereal samples can be recorded. For light microscopy, samples are usually fixed, embedded and cut into sections for staining with different dyes in order to visualize various components present in cereals. Less sample preparation is needed for confocal laser scanning microscopy (CLSM), which enables optical sectioning of the sample. Scanning electron microscopy (SEM) gives information on the structure with higher magnification, when high resolution is required. Depending on which structural level is of interest, these techniques can be used in revealing the structural differences between processes, enzymatic treatments, etc. Several examples are presented on the visualization of grain structures and components and their changes due to processing.
KW - stereomicroscopy
KW - light microscopy
KW - brightfield microscopy
KW - fluorescence microscopy
KW - confocal laser scanning microscopy
KW - scanning electron microscopy
U2 - 10.1007/978-3-319-24735-9_1
DO - 10.1007/978-3-319-24735-9_1
M3 - Chapter or book article
SN - 978-3-319-24733-5
T3 - Food Engineering Series
SP - 1
EP - 39
BT - Imaging Technologies and Data Processing for Food Engineers
A2 - Sozer, Nesli
PB - Springer
ER -