Changes in wheat micro structure following in vitro digestion

Teija Parkkonen, Asta Tervilä-Wilo, Maarit Hopeakoski-Nurminen, Andrew Morgan, Kaisa Poutanen, Karin Autio

Research output: Contribution to journalArticleScientificpeer-review

36 Citations (Scopus)

Abstract

A three‐step digestion simulation model was used to study the effects of monogastric digestive enzymes and exogenous microbial enzymes on the microstructure of wheat. Sections were stained for cell wall polysaccharides, protein, starch and phytate. Bright field and fluorescence microscopy was used to study the microstructural changes following each step of the digestion process. Starchy endosperm protein was not detected after the pepsin step, although aleurone protein was largely unaffected. After pancreatin treatment, starch had also disappeared. Xylanase decreased the fluorescence of the aleurone protein and collapsed the cell walls in the subaleurone. Unbroken cell walls of the aleurone and endosperm were not influenced by any of the treatments.

Original languageEnglish
Pages (from-to)43 - 47
Number of pages5
JournalActa agriculturæ Scandinavica Section B: Soil and plant science
Volume47
Issue number1
DOIs
Publication statusPublished - 1997
MoE publication typeA1 Journal article-refereed

Fingerprint

in vitro digestion
microstructure
digestion
wheat
protein
cell walls
starch
endosperm
fluorescence
proteins
enzyme
pancreatin
monogastric livestock
digestive enzymes
xylanases
pepsin
fluorescence microscopy
phytic acid
polysaccharide
microscopy

Cite this

Parkkonen, Teija ; Tervilä-Wilo, Asta ; Hopeakoski-Nurminen, Maarit ; Morgan, Andrew ; Poutanen, Kaisa ; Autio, Karin. / Changes in wheat micro structure following in vitro digestion. In: Acta agriculturæ Scandinavica Section B: Soil and plant science. 1997 ; Vol. 47, No. 1. pp. 43 - 47.
@article{ce24626b738c4fa1af66b78b35e854cc,
title = "Changes in wheat micro structure following in vitro digestion",
abstract = "A three‐step digestion simulation model was used to study the effects of monogastric digestive enzymes and exogenous microbial enzymes on the microstructure of wheat. Sections were stained for cell wall polysaccharides, protein, starch and phytate. Bright field and fluorescence microscopy was used to study the microstructural changes following each step of the digestion process. Starchy endosperm protein was not detected after the pepsin step, although aleurone protein was largely unaffected. After pancreatin treatment, starch had also disappeared. Xylanase decreased the fluorescence of the aleurone protein and collapsed the cell walls in the subaleurone. Unbroken cell walls of the aleurone and endosperm were not influenced by any of the treatments.",
author = "Teija Parkkonen and Asta Tervil{\"a}-Wilo and Maarit Hopeakoski-Nurminen and Andrew Morgan and Kaisa Poutanen and Karin Autio",
year = "1997",
doi = "10.1080/09064719709362437",
language = "English",
volume = "47",
pages = "43 -- 47",
journal = "Acta agricultur{\ae} Scandinavica Section B: Soil and plant science",
issn = "0906-4710",
publisher = "Taylor & Francis",
number = "1",

}

Changes in wheat micro structure following in vitro digestion. / Parkkonen, Teija; Tervilä-Wilo, Asta; Hopeakoski-Nurminen, Maarit; Morgan, Andrew; Poutanen, Kaisa; Autio, Karin.

In: Acta agriculturæ Scandinavica Section B: Soil and plant science, Vol. 47, No. 1, 1997, p. 43 - 47.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Changes in wheat micro structure following in vitro digestion

AU - Parkkonen, Teija

AU - Tervilä-Wilo, Asta

AU - Hopeakoski-Nurminen, Maarit

AU - Morgan, Andrew

AU - Poutanen, Kaisa

AU - Autio, Karin

PY - 1997

Y1 - 1997

N2 - A three‐step digestion simulation model was used to study the effects of monogastric digestive enzymes and exogenous microbial enzymes on the microstructure of wheat. Sections were stained for cell wall polysaccharides, protein, starch and phytate. Bright field and fluorescence microscopy was used to study the microstructural changes following each step of the digestion process. Starchy endosperm protein was not detected after the pepsin step, although aleurone protein was largely unaffected. After pancreatin treatment, starch had also disappeared. Xylanase decreased the fluorescence of the aleurone protein and collapsed the cell walls in the subaleurone. Unbroken cell walls of the aleurone and endosperm were not influenced by any of the treatments.

AB - A three‐step digestion simulation model was used to study the effects of monogastric digestive enzymes and exogenous microbial enzymes on the microstructure of wheat. Sections were stained for cell wall polysaccharides, protein, starch and phytate. Bright field and fluorescence microscopy was used to study the microstructural changes following each step of the digestion process. Starchy endosperm protein was not detected after the pepsin step, although aleurone protein was largely unaffected. After pancreatin treatment, starch had also disappeared. Xylanase decreased the fluorescence of the aleurone protein and collapsed the cell walls in the subaleurone. Unbroken cell walls of the aleurone and endosperm were not influenced by any of the treatments.

U2 - 10.1080/09064719709362437

DO - 10.1080/09064719709362437

M3 - Article

VL - 47

SP - 43

EP - 47

JO - Acta agriculturæ Scandinavica Section B: Soil and plant science

JF - Acta agriculturæ Scandinavica Section B: Soil and plant science

SN - 0906-4710

IS - 1

ER -