Characterisation of fractions obtained by isoamylolysis and ion-exchange chromatography of cationic waxy maize starch

Robin Manelius, Hannu Maaheimo, Kari Nurmi, Eric Bertoft (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

12 Citations (Scopus)

Abstract

Isoamylase hydrolysates of wet- (WC) and dry-cationised (DC) waxy maize starch were fractionated by ion-exchange chromatography on CM-Sepharose into an unbound and four bound fractions. The amount of bound dextrins was higher in the WC than in the DC sample. The fractions were characterised by gel-permeation chromatography and proton-NMR spectroscopy. The unbound fraction from the WC sample consisted mainly of linear chains formed from amylopectin. The dextrins in the bound fractions contained increased amounts, from 1.2 - 7.9, of cationic substituents per molecule and the degree of polymerisation increased with the density of substituents. Dextrins weakly bound to CM-Sepharose had a linear structure, whereas more tightly bound fractions were mixtures of linear and branched dextrins. In the latter, the debranching was incomplete because of sterical hindrance by substituents at or close to the branch points. Most of the dextrins were partly hydrolysed by beta-amylase, but the more highly substituted fractions possessed also a population of -amylase resistant dextrins, suggesting substituents at the non-reducing ends.
Original languageEnglish
Pages (from-to)58-65
JournalStärke
Volume54
Issue number2
DOIs
Publication statusPublished - 2002
MoE publication typeA1 Journal article-refereed

Fingerprint

Dextrins
waxy corn
dextrins
Ion Exchange Chromatography
ion exchange chromatography
Chromatography
corn starch
Starch
Zea mays
Ion exchange
Sepharose
agarose
Isoamylase
beta-Amylase
isoamylase
Amylopectin
beta-amylase
amylopectin
Gel permeation chromatography
Amylases

Keywords

  • cationic starch
  • waxy maize starch
  • ion-exchange chromatography
  • isoamylolysis

Cite this

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title = "Characterisation of fractions obtained by isoamylolysis and ion-exchange chromatography of cationic waxy maize starch",
abstract = "Isoamylase hydrolysates of wet- (WC) and dry-cationised (DC) waxy maize starch were fractionated by ion-exchange chromatography on CM-Sepharose into an unbound and four bound fractions. The amount of bound dextrins was higher in the WC than in the DC sample. The fractions were characterised by gel-permeation chromatography and proton-NMR spectroscopy. The unbound fraction from the WC sample consisted mainly of linear chains formed from amylopectin. The dextrins in the bound fractions contained increased amounts, from 1.2 - 7.9, of cationic substituents per molecule and the degree of polymerisation increased with the density of substituents. Dextrins weakly bound to CM-Sepharose had a linear structure, whereas more tightly bound fractions were mixtures of linear and branched dextrins. In the latter, the debranching was incomplete because of sterical hindrance by substituents at or close to the branch points. Most of the dextrins were partly hydrolysed by beta-amylase, but the more highly substituted fractions possessed also a population of -amylase resistant dextrins, suggesting substituents at the non-reducing ends.",
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Characterisation of fractions obtained by isoamylolysis and ion-exchange chromatography of cationic waxy maize starch. / Manelius, Robin; Maaheimo, Hannu; Nurmi, Kari; Bertoft, Eric (Corresponding Author).

In: Stärke, Vol. 54, No. 2, 2002, p. 58-65.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Characterisation of fractions obtained by isoamylolysis and ion-exchange chromatography of cationic waxy maize starch

AU - Manelius, Robin

AU - Maaheimo, Hannu

AU - Nurmi, Kari

AU - Bertoft, Eric

PY - 2002

Y1 - 2002

N2 - Isoamylase hydrolysates of wet- (WC) and dry-cationised (DC) waxy maize starch were fractionated by ion-exchange chromatography on CM-Sepharose into an unbound and four bound fractions. The amount of bound dextrins was higher in the WC than in the DC sample. The fractions were characterised by gel-permeation chromatography and proton-NMR spectroscopy. The unbound fraction from the WC sample consisted mainly of linear chains formed from amylopectin. The dextrins in the bound fractions contained increased amounts, from 1.2 - 7.9, of cationic substituents per molecule and the degree of polymerisation increased with the density of substituents. Dextrins weakly bound to CM-Sepharose had a linear structure, whereas more tightly bound fractions were mixtures of linear and branched dextrins. In the latter, the debranching was incomplete because of sterical hindrance by substituents at or close to the branch points. Most of the dextrins were partly hydrolysed by beta-amylase, but the more highly substituted fractions possessed also a population of -amylase resistant dextrins, suggesting substituents at the non-reducing ends.

AB - Isoamylase hydrolysates of wet- (WC) and dry-cationised (DC) waxy maize starch were fractionated by ion-exchange chromatography on CM-Sepharose into an unbound and four bound fractions. The amount of bound dextrins was higher in the WC than in the DC sample. The fractions were characterised by gel-permeation chromatography and proton-NMR spectroscopy. The unbound fraction from the WC sample consisted mainly of linear chains formed from amylopectin. The dextrins in the bound fractions contained increased amounts, from 1.2 - 7.9, of cationic substituents per molecule and the degree of polymerisation increased with the density of substituents. Dextrins weakly bound to CM-Sepharose had a linear structure, whereas more tightly bound fractions were mixtures of linear and branched dextrins. In the latter, the debranching was incomplete because of sterical hindrance by substituents at or close to the branch points. Most of the dextrins were partly hydrolysed by beta-amylase, but the more highly substituted fractions possessed also a population of -amylase resistant dextrins, suggesting substituents at the non-reducing ends.

KW - cationic starch

KW - waxy maize starch

KW - ion-exchange chromatography

KW - isoamylolysis

U2 - 10.1002/1521-379X(200202)54:2<58::AID-STAR58>3.0.CO;2-1

DO - 10.1002/1521-379X(200202)54:2<58::AID-STAR58>3.0.CO;2-1

M3 - Article

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SN - 2192-4236

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